LOCUS       sequence-252948-       10415 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level1 ZmUBIp::LbCas12a (rice codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH61
FEATURES             Location/Qualifiers
     source          1..10415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2029..2049
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2074..5754
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             5755..5802
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5818..6070
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6118..6142
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6298..7446)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(7782..8370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7862..7881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8541..9332,9333..9401))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9164..9183
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9402..9506)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9593..10224
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10316..10340
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatggctcc taagaagaag
     2041 cggaaggttg gtattcacgg ggtgcctgcg gcttcaaagc tcgagaaatt caccaactgt
     2101 tattcgttga gcaaaacact gcggtttaaa gcgattccag tcggcaagac tcaagagaat
     2161 atagacaata agcggctgtt ggtggaagat gaaaagcgcg cggaagatta caaaggggtg
     2221 aagaagttgt tggacagata ctacctctct tttatcaatg atgtcttgca ctcaatcaaa
     2281 ttgaagaatc tgaacaacta catctccctc ttcagaaaga aaacaaggac agaaaaggag
     2341 aataaggaac ttgaaaattt ggagatcaat ctgaggaaag agatcgcgaa agcctttaaa
     2401 ggcaacgaag gatacaaaag tctgttcaag aaggatataa ttgagacaat tttgccagag
     2461 ttcctcgatg acaaggacga gattgcgctg gtcaattcgt tcaacggatt cacaacagca
     2521 ttcacaggct tctttgataa tcgggaaaat atgttctctg aggaggcaaa gtccacttct
     2581 attgcgttca ggtgtatcaa tgagaatctc actaggtaca tttccaacat ggatatcttt
     2641 gagaaggttg acgcaatttt tgacaagcac gaagttcagg agattaagga gaagatcctc
     2701 aattccgatt atgacgttga ggacttcttc gaaggtgagt tttttaattt cgtgctcact
     2761 caagagggta tcgacgtgta taatgcgatc atcggtgggt tcgtgactga gtccggtgaa
     2821 aagattaagg gattgaacga gtatatcaac ctttacaacc aaaagacgaa acagaagctg
     2881 ccaaagttca agcctcttta caaacaggtt ctttcagacc gcgagtcact ctcgttctat
     2941 ggggagggct acacttcgga tgaggaagtc ctggaggtgt tcaggaatac tctcaataag
     3001 aattcggaga ttttctcttc tataaaaaaa ctggaaaagt tgtttaagaa ttttgacgaa
     3061 tactctagcg ccggcatatt tgtgaaaaac ggcccggcca tatcaacgat aagtaaagat
     3121 atcttcggcg aatggaacgt gatcagagac aaatggaacg cggagtatga cgatattcac
     3181 ctgaagaaga aggctgtcgt aacggagaag tacgaggatg atcgcaggaa aagcttcaaa
     3241 aagatcggaa gtttcagcct ggaacagttg caggagtatg ctgacgccga tcttagcgtc
     3301 gtcgagaagt tgaaggagat aatcatccaa aaggtcgacg agatatataa agtctatgga
     3361 tcaagtgaaa aactgttcga cgccgacttc gttttggaga agtccctgaa gaagaacgac
     3421 gctgttgttg ccattatgaa ggatctgctc gacagcgtga agagtttcga gaactatatt
     3481 aaggcttttt tcggggaggg gaaggagact aacagagatg agtccttcta cggagacttc
     3541 gtcctcgcgt acgatatact ccttaaggta gaccacatct acgacgcaat cagaaattac
     3601 gtgacacaaa agccgtacag caaggacaag ttcaaactct acttccagaa cccccagttc
     3661 atgggcggct gggacaagga caaggaaacg gattacaggg ctacgatcct gaggtatggt
     3721 tcaaaatact acttggcgat tatggacaag aagtacgcca agtgtctcca gaagattgac
     3781 aaagacgatg tcaatggcaa ttatgagaag atcaactaca agctgcttcc gggtccgaac
     3841 aagatgctcc caaaggtttt cttcagcaag aaatggatgg cctactataa cccaagcgag
     3901 gacatccaga agatttataa gaacggtacg ttcaagaagg gcgacatgtt caatcttaac
     3961 gactgtcaca agctgatcga cttcttcaaa gactcaatta gccggtaccc aaagtggtct
     4021 aacgcctatg acttcaactt ttcggaaacc gagaagtaca aggatatagc cggattttat
     4081 agagaggtgg aagagcaggg ctacaaggtg tcattcgagt ccgccagcaa gaaggaagtg
     4141 gacaagctcg tggaagaggg taagctctac atgttccaga tttataataa agactttagc
     4201 gataagagcc acgggacacc taatctccac acaatgtatt tcaagctgct cttcgacgag
     4261 aataaccacg gccaaatcag gttgtcagga ggggctgaac tcttcatgcg gcgcgctagc
     4321 cttaagaagg aggagcttgt agtccaccct gcgaatagtc caattgcgaa taagaacccg
     4381 gacaatccta aaaagactac aacattgagc tacgacgtgt acaaggataa gaggttttcc
     4441 gaggatcagt acgagctcca catcccgatt gcgatcaaca agtgcccaaa gaatattttc
     4501 aagataaaca cagaggtgcg tgtactcctg aagcatgacg acaatcctta cgtcattggg
     4561 attgatcggg gcgagaggaa cctcctctat attgtggtgg tggacgggaa ggggaacata
     4621 gtcgaacagt actcccttaa cgaaataatt aacaatttca acggcatccg tatcaagacc
     4681 gactaccatt cgttgctgga caagaaggag aaggagagat ttgaggcgcg gcaaaattgg
     4741 acaagtatcg agaacatcaa ggaactcaaa gcaggttata tctctcaagt tgtgcataag
     4801 atatgcgagc tggttgagaa gtatgacgca gtgatcgctc ttgaggacct caactcgggc
     4861 tttaagaatt ctagagttaa agtggagaag caggtctatc aaaagttcga gaagatgctt
     4921 atagataagc tcaactacat ggtcgataag aaatcgaacc catgtgccac cggcggcgca
     4981 ctcaaaggtt accaaataac aaacaaattc gagtccttca aatcgatgag tactcagaat
     5041 gggttcatat tttatatacc ggcgtggctt acgtctaaga tcgacccgtc aactggtttt
     5101 gtcaacctgt taaagacgaa atacacgtcc attgccgatt cgaaaaagtt catatctagt
     5161 tttgatcgta ttatgtacgt cccagaggaa gatcttttcg agtttgctct cgactacaaa
     5221 aacttttcgc ggaccgatgc ggattacatt aaaaaatgga aactctattc gtacggcaac
     5281 agaatcagga tttttcgcaa ccctaagaag aataacgtct ttgattggga ggaagtttgc
     5341 ttgactagcg cgtacaagga gctctttaat aagtatggca ttaactacca acagggtgat
     5401 atcagagcac tgctttgcga acaatctgac aaggctttct actcatcctt catggctttg
     5461 atgagcctga tgctccagat gagaaattca attacaggca gaaccgacgt ggatttcttg
     5521 atctccccgg ttaaaaattc tgatggcatc ttttacgata gcaggaacta tgaagcgcaa
     5581 gagaatgcga ttctgccaaa aaatgcagac gccaacggtg cctataacat cgccaggaaa
     5641 gtcctgtggg cgatcggcca gttcaaaaag gccgaggacg aaaaattgga caaggtcaaa
     5701 atcgctatca gcaacaaaga gtggctggag tatgctcaga catccgtaaa gcataagcgt
     5761 cctgctgcca ccaaaaaggc cggacaggct aagaaaaaga agtgagcttg tcaagcagat
     5821 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     5881 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     5941 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     6001 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     6061 ttactagatc gacgctacta ttgtcttctg cacgaagtgg tttaaactat cagtgtttga
     6121 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     6181 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     6241 ttccccagat caggcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta
     6301 gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg
     6361 caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga
     6421 tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa
     6481 atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt
     6541 cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg
     6601 ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag
     6661 gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc
     6721 ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta
     6781 gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg
     6841 ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc
     6901 cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg
     6961 cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca
     7021 cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag
     7081 caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg
     7141 cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc
     7201 ctcctgttca agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg
     7261 agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac
     7321 ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc
     7381 ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg
     7441 attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc
     7501 ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc
     7561 ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac
     7621 gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca
     7681 cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta
     7741 ggatctgcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7801 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7861 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7921 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7981 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8041 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8101 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8161 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8221 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8281 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8341 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8401 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8461 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8521 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8581 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8641 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
     8701 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8761 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8821 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8881 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8941 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9001 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9061 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9121 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9181 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9241 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9301 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9361 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9421 tattgaagca tttatcaagg ttattgtctc atgagcggat acatatttga atgtatttag
     9481 aaaaataaac aaataggggt tccgcgcacg aattggccag cgctgccatt tttggggtga
     9541 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
     9601 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
     9661 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
     9721 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
     9781 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
     9841 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
     9901 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
     9961 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
    10021 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca
    10081 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac
    10141 ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg
    10201 agggcaacca gcccggtgag cgtcgcaaag gagatcctga tctgactgat gggctgcctg
    10261 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
    10321 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
    10381 ttttaatgta ctggggtgga tgcagtgggc cccac
//
