LOCUS       sequence-252973-       11708 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of RET V648I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-RET-V648I
FEATURES             Location/Qualifiers
     source          1..11708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6668..6692)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6700..6718
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6743..6762)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6744..6762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6768..6788)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6842..6883
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6904..7471
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6921..7473
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7065..7082)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7292..7311
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7485..8084
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7485..7504)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7981..8001
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8098..8686
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8151..8171)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8569..8580)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8761..8994
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9113..9131)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9199..9303
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9304..9372,9373..10164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9522..9541)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10335..10923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10824..10843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11077..11094
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11334..11355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11370..11375,11376..11393,11394..11400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11408..11424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11413..11435
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11432..11448
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11432..11448
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11498..11517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcgaaggcga cgtccggtgc cgcggggctg
     3481 cgtctgctgt tgctgctgct gctgccgctg ctaggcaaag tggcattggg cctctacttc
     3541 tcgagggatg cttactggga gaagctgtat gtggaccagg cggccggcac gcccttgctg
     3601 tacgtccatg ccctgcggga cgcccctgag gaggtgccca gcttccgcct gggccagcat
     3661 ctctacggca cgtaccgcac acggctgcat gagaacaact ggatctgcat ccaggaggac
     3721 accggcctcc tctaccttaa ccggagcctg gaccatagct cctgggagaa gctcagtgtc
     3781 cgcaaccgcg gctttcccct gctcaccgtc tacctcaagg tcttcctgtc acccacatcc
     3841 cttcgtgagg gcgagtgcca gtggccaggc tgtgcccgcg tatacttctc cttcttcaac
     3901 acctcctttc cagcctgcag ctccctcaag ccccgggagc tctgcttccc agagacaagg
     3961 ccctccttcc gcattcggga gaaccgaccc ccaggcacct tccaccagtt ccgcctgctg
     4021 cctgtgcagt tcttgtgccc caacatcagc gtggcctaca ggctcctgga gggtgagggt
     4081 ctgcccttcc gctgcgcccc ggacagcctg gaggtgagca cgcgctgggc cctggaccgc
     4141 gagcagcggg agaagtacga gctggtggcc gtgtgcaccg tgcacgccgg cgcgcgcgag
     4201 gaggtggtga tggtgccctt cccggtgacc gtgtacgacg aggacgactc ggcgcccacc
     4261 ttccccgcgg gcgtcgacac cgccagcgcc gtggtggagt tcaagcggaa ggaggacacc
     4321 gtggtggcca cgctgcgtgt cttcgatgca gacgtggtac ctgcatcagg ggagctggtg
     4381 aggcggtaca caagcacgct gctccccggg gacacctggg cccagcagac cttccgggtg
     4441 gaacactggc ccaacgagac ctcggtccag gccaacggca gcttcgtgcg ggcgaccgta
     4501 catgactata ggctggttct caaccggaac ctctccatct cggagaaccg caccatgcag
     4561 ctggcggtgc tggtcaatga ctcagacttc cagggcccag gagcgggcgt cctcttgctc
     4621 cacttcaacg tgtcggtgct gccggtcagc ctgcacctgc ccagtaccta ctccctctcc
     4681 gtgagcagga gggctcgccg atttgcccag atcgggaaag tctgtgtgga aaactgccag
     4741 gcattcagtg gcatcaacgt ccagtacaag ctgcattcct ctggtgccaa ctgcagcacg
     4801 ctaggggtgg tcacctcagc cgaggacacc tcggggatcc tgtttgtgaa tgacaccaag
     4861 gccctgcggc ggcccaagtg tgccgaactt cactacatgg tggtggccac cgaccagcag
     4921 acctctaggc aggcccaggc ccagctgctt gtaacagtgg aggggtcata tgtggccgag
     4981 gaggcgggct gccccctgtc ctgtgcagtc agcaagagac ggctggagtg tgaggagtgt
     5041 ggcggcctgg gctccccaac aggcaggtgt gagtggaggc aaggagatgg caaagggatc
     5101 accaggaact tctccacctg ctctcccagc accaagacct gccccgacgg ccactgcgat
     5161 gttgtggaga cccaagacat caacatttgc cctcaggact gcctccgggg cagcattgtt
     5221 gggggacacg agcctgggga gccccggggg attaaagctg gctatggcac ctgcaactgc
     5281 ttccctgagg aggagaagtg cttctgcgag cccgaagaca tccaggatcc actgtgcgac
     5341 gagctgtgcc gcacggtgat cgcagccgct gtcctcttct ccttcatcat ctcggtgctg
     5401 ctgtctgcct tctgcatcca ctgctaccac aagtttgccc acaagccacc catctcctca
     5461 gctgagatga ccttccggag gcccgcccag gccttcccgg tcagctactc ctcttccagt
     5521 gcccgccggc cctcgctgga ctccatggag aaccaggtct ccgtggatgc cttcaagatc
     5581 ctggaggatc caaagtggga attccctcgg aagaacttgg ttcttggaaa aactctagga
     5641 gaaggcgaat ttggaaaagt ggtcaaggca acggccttcc atctgaaagg cagagcaggg
     5701 tacaccacgg tggccgtgaa gatgctgaaa gagaacgcct ccccgagtga gcttcgagac
     5761 ctgctgtcag agttcaacgt cctgaagcag gtcaaccacc cacatgtcat caaattgtat
     5821 ggggcctgca gccaggatgg cccgctcctc ctcatcgtgg agtacgccaa atacggctcc
     5881 ctgcggggct tcctccgcga gagccgcaaa gtggggcctg gctacctggg cagtggaggc
     5941 agccgcaact ccagctccct ggaccacccg gatgagcggg ccctcaccat gggcgacctc
     6001 atctcatttg cctggcagat ctcacagggg atgcagtatc tggccgagat gaagctcgtt
     6061 catcgggact tggcagccag aaacatcctg gtagctgagg ggcggaagat gaagatttcg
     6121 gatttcggct tgtcccgaga tgtttatgaa gaggattcgt acgtgaagag gagccagggt
     6181 cggattccag ttaaatggat ggcaattgaa tccctttttg atcatatcta caccacgcaa
     6241 agtgatgtat ggtcttttgg tgtcctgctg tgggagatcg tgaccctagg gggaaacccc
     6301 tatcctggga ttcctcctga gcggctcttc aaccttctga agaccggcca ccggatggag
     6361 aggccagaca actgcagcga ggagatgtac tgcctgatgc tgcaatgctg gaagcaggag
     6421 ccggacaaaa ggccggtgtt tgcggacatc agcaaagacc tggagaagat gatggttaag
     6481 aggagagact acttggacct tgcggcgtcc actccatctg actccctgat ttatgacgac
     6541 ggcctctcag aggaggagac accgctggtg gactgtaata atgcccccct ccctcgagcc
     6601 ctcccttcca catggattga aaacaaactc tatggtagaa tttcccatgc atttactaga
     6661 ttctagaacc cagctttctt gtacaaagtg gtgatatcca attaaccctc actaaaggtt
     6721 taagtgagtt atagaagtag taccctatag tgagtcgtat taccacccaa cttttctata
     6781 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     6841 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg
     6901 agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     6961 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     7021 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     7081 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     7141 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     7201 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     7261 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     7321 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     7381 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     7441 cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgacc gagtacaagc
     7501 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     7561 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     7621 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7681 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7741 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7801 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7861 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7921 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     7981 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8041 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctaaatcgat agatcctaat
     8101 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8161 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8221 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8281 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8341 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8401 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8461 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8521 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8581 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8641 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     8701 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8761 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8821 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8881 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8941 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     9001 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9061 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     9121 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     9181 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9241 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9301 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9361 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9421 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9481 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9541 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9601 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9661 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9721 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9781 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9841 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9901 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9961 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    10021 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10081 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10141 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10201 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10261 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10321 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10381 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10441 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    10501 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10561 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10621 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10681 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    10741 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10801 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10861 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10921 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10981 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    11041 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    11101 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    11161 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    11221 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
    11281 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    11341 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    11401 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
    11461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    11521 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    11581 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    11641 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    11701 ttagtagt
//
