LOCUS       sequence-253566-        3357 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC38A10
FEATURES             Location/Qualifiers
     source          1..3357
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..151)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
ORIGIN
        1 atgaccgcag cagcagcaag caactgggga ctgatcacaa acatcgtgaa ttctatcgtg
       61 ggcgtgagcg tgctgaccat gcccttctgc tttaagcagt gtggaatcgt gctgggcgcc
      121 ctgctgctgg tgttttgctc ttggatgacc caccagagct gtatgttcct ggtgaagtct
      181 gccagcctgt ccaagcggag aacatacgcc ggcctggcct ttcatgcgta tggaaaggcg
      241 gggaagatgc tggtggagac cagcatgatc ggcctgatgc tgggcacatg catcgccttc
      301 tatgtggtca tcggcgacct gggctccaac ttctttgcca gactgttcgg ctttcaagtg
      361 ggcggcacct tcaggatgtt tctgctgttc gccgtgagcc tgtgcatcgt gctgcctctg
      421 tccctgcaga gaaatatgat ggcctctatc cagtctttta gcgccatggc cctgctgttc
      481 tacacagtgt tcatgtttgt gatcgtgctg agctccctga agcacggcct gtttagcggc
      541 cagtggctga ggcgcgtgtc ctatgtgcgc tgggagggcg tgttccggtg catcccaatc
      601 tttggcatgt ccttcgcctg tcagtctcag gtgctgccca cctacgacag cctggatgag
      661 ccttccgtga agacaatgtc tagcatcttt gcctcctctc tgaatgtggt gaccacattc
      721 tacgtgatgg tgggcttctt tggctatgtg agctttaccg aggccacagc cggcaacgtg
      781 ctgatgcact tcccctccaa tctggtgacc gagatgctgc gggtgggctt tatgatgtct
      841 gtggccgtgg gcttccccat gatgatcctg ccttgcagac aggccctgtc caccctgctg
      901 tgcgagcagc agcagaagga cggcacattt gccgcaggag gatatatgcc acctctgcgg
      961 tttaaggccc tgaccctgag cgtggtgttc ggcacaatgg tgggcggcat cctgatccca
     1021 aacgtggaga ccatcctggg actgaccgga gcaacaatgg gatccctgat ctgcttcatc
     1081 tgtcccgccc tgatctacaa gaagatccac aagaatgccc tgagctccca ggtggtgctg
     1141 tgggtgggac tgggcgtgct ggtggtgagc acagtgacca cactgtccgt gtctgaggaa
     1201 gtgccagagg atctggcaga ggaggcacca ggaggcagac tgggcgaggc agagggactg
     1261 atgaaggtgg aggcagccag gctgtccgcc caggacccag tggtggcagt ggcagaggat
     1321 ggccgcgaga agccaaagct gccaaaggag agggaggagc tggagcaggc ccagatcaag
     1381 ggacctgtgg acgtgccagg aagggaggat ggcaaggagg cacctgagga ggcacagctg
     1441 gacaggccag gccagggcat cgccgtgccc gtgggcgagg cccacagaca cgagccaccc
     1501 gtgcctcacg ataaggtggt ggtggacgag ggacaggata gggaggtgcc agaggagaac
     1561 aagcctccat ctaggcacgc aggaggcaag gcaccaggag tgcagggcca gatggccccc
     1621 cctctgccag acagcgagag ggagaagcag gagcctgagc agggcgaagt gggcaagcgg
     1681 ccaggacagg cacaggccct ggaggaggca ggcgacctgc ctgaggatcc acagaaggtg
     1741 cctgaggcag atggacagcc agcagtgcag cctgcaaagg aggacctggg accaggcgat
     1801 agaggactgc acccaaggcc tcaggccgtg ctgtccgagc agcagaacgg actggcagtg
     1861 ggaggaggag agaaggccaa gggaggacca ccacctggaa atgcagcagg cgacaccggc
     1921 cagccagccg aggactctga tcacggcggc aagccaccac tgccagcaga gaagccagca
     1981 ccaggacctg gcctgcctcc agagccaagg gagcagaggg atgtggagag ggcaggagga
     2041 aatcaggcag caagccagct ggaggaggca ggacgcgccg agatgctgga ccacgccgtg
     2101 ctgctgcagg tcatcaagga gcagcaggtg cagcagaagc ggctgctgga tcagcaggag
     2161 aagctgctgg ccgtgatcga ggagcagcac aaggagatcc accagcagag gcaggaggac
     2221 gaggaggata agccaaggca ggtggaggtg caccaggagc caggagcagc agtgccaagg
     2281 ggacaggagg cacctgaggg caaggcaagg gagaccgtgg agaacctgcc acctctgcca
     2341 ctggaccctg tgctgagggc accaggaggc cggccagcac cctcccagga tctgaatcag
     2401 agaagcctgg agcactccga gggccctgtg ggcagggacc cagcaggacc acctgacgga
     2461 ggaccagata ccgagcccag agcagcacag gcaaagctga gggacggaca gaaggatgca
     2521 gcaccaaggg cagccggcac agtgaaggag ctgccaaagg gaccagagca ggtgcctgtg
     2581 ccagatccag caagagaggc aggaggacca gaggagaggc tggcagagga gttccctgga
     2641 cagtcccagg acgtgaccgg aggctctcag gatcgcaaga agcctggcaa ggaggtggca
     2701 gcaaccggca catccatcct gaaggaggca aactggctgg tggcaggacc aggagcagag
     2761 acaggcgacc ctcgcatgaa gccaaagcag gtgtctcggg acctgggcct ggccgccgat
     2821 ctgccaggag gagcagaggg agcagcagca cagcctcagg ccgtgctgag acagccagag
     2881 ctgagagtga tctctgacgg agagcaggga ggacagcagg gacacaggct ggatcacggc
     2941 ggccacctgg agatgagaaa ggcaagggga ggcgaccacg tgcccgtgag ccacgagcag
     3001 cctaggggag gcgaggatgc cgccgtgcag gagcctagac agaggcctga gccagagctg
     3061 ggcctgaaga gggcagtgcc aggaggacag aggcctgaca acgccaagcc aaatcgggat
     3121 ctgaagctgc aggcaggaag cgacctgcgg agaaggcgcc gggatctggg acctcacgca
     3181 gagggacagc tggcaccaag agacggcgtg atcatcggcc tgaaccccct gcctgacgtg
     3241 caagtgaatg atctgagagg cgccctggat gcacagctga ggcaggcagc aggaggcgcc
     3301 ctgcaggtgg tgcactctcg ccagctgcgg caggcccccg gccctccaga ggagagc
//
