LOCUS       sequence-254530-        3828 bp    DNA     circular SYN 14-APR-2025
DEFINITION  CmYLCV::STM::35S terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pRN110
FEATURES             Location/Qualifiers
     source          1..3828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1404..1578
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        1647..1751
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2819..3407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3308..3327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3561..3578
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccaataatgg agcaatgacg taagggctta cgaaataagt aagggtagtt tgggaaatgt
       61 ccactcaccc gtcagtctat aaatacttag cccctccctc attgttaagg gagcaaaatc
      121 tcagagagat agtcctagag agagaaagag agcaagtagc ctagaagtag tcaaggcggc
      181 gaagtattca ggcacgtggc caggaagaag aaaagccaag acgacgaaaa caggtaagag
      241 ctaagcttat ggagagtggt tccaacagca cttcttgtcc aatggctttt gccggggata
      301 atagtgatgg tccgatgtgt cctatgatga tgatgatgcc gcccatcatg acatcacatc
      361 aacatcatgg tcatgatcat caacatcaac aacaagaaca tgatggttat gcatatcagt
      421 cacaccacca acaaagtagt tccctttttc ttcaatcact agctcctccc caaggaacta
      481 agaacaaagt tgcttcttct tcttctcctt cctcttgtgc tcctgcctat tctctaatgg
      541 agatccatca taacgaaatc gttgcaggag gaatcaaccc ttgctcctct tcctcttctt
      601 cagcctctgt caaggccaag atcatggctc atcctcacta ccaccgcctc ttggccgctt
      661 atgtcaattg tcagaaggtt ggagcaccac cggaggttgt ggcgaggcta gaggaggcat
      721 gctcgtctgc cgcagccgct gccgcatcta tgggaccaac aggatgtcta ggtgaagatc
      781 cagggcttga tcaattcatg gaagcttact gtgaaatgct cgttaagtat gagcaagagc
      841 tctccaaacc tttcaaggaa gctatggtct tccttcaacg tgtcgagtgt caattcaaat
      901 ccctctctct atcctcacct tcctctttct ccggttatgg agagacagca attgatagga
      961 acaataatgg gtcatccgag gaagaagtcg atatgaacaa tgaatttgta gatccacaag
     1021 ctgaggatag agagcttaaa ggacagctct tgcgcaagta cagtggttac ttagggagcc
     1081 tcaagcaaga gttcatgaag aagaggaaga aaggaaagct ccctaaagaa gctcgtcaac
     1141 aactgcttga ttggtggagc cgtcactaca aatggcctta cccttcggag caacaaaagc
     1201 tcgcccttgc ggaatcaacg gggctggacc agaaacagat aaacaattgg ttcataaacc
     1261 agaggaaacg gcattggaag ccgtcggagg acatgcagtt tgtagtaatg gacgcaacac
     1321 atcctcacca ttacttcatg gataatgtct tgggcaatcc tttcccaatg gatcacatct
     1381 cctccaccat gctttgactc gagtttctcc ataataatgt gtgagtagtt cccagataag
     1441 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     1501 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     1561 agtactaaaa tccagatccc ccgaattaag tgactagcag gtgatgaaaa gccttagggt
     1621 caggtggcac ttttcgggga aatgtgcgat gaacccctat ttgtttattt ttctaaatac
     1681 attcaaatat gtatccgcta atgagacaat aaccctgata aatgcttcaa ttatattgaa
     1741 aaaggaagag tatgagtatc caacatttcc gtgtcgccct tattcccttt tttgcggcct
     1801 tctgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1861 agttgggtgc ccgagtgggt tacattgaac tggatctcaa cagcggcaaa atcttagaga
     1921 gttttcgccc cgaagaacgc ttcccaatga tgagcacttt caaagttctg ctatgtggcg
     1981 cggtgttatc ccgtattgat gctgggcaag agcaactcgg tcgccgcata cactattctc
     2041 agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2101 taagagaatt atgtagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2161 tgacaacgat tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2221 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2281 acaccacgat gcctgtagca atggcaacaa ctctccgcaa actattaact ggcgaactac
     2341 ttactcttgc ttcacgccag caactcattg actggatgga ggcggacaaa gttgcaggac
     2401 cacttctgcg ctcggcactt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2461 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2521 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2581 agataggtgc ctcactgatt aagcattggt aactgtcaga cgaagggaaa taaatagtag
     2641 cccgcctgat gtgcgggctt ttttttggtg gtggttacca gtgtcgctta gctaccgtag
     2701 gtcctcttag gcggtccgat tagcgacata gtcatcaatg ccttgacggc aaaatccctt
     2761 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2821 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2881 cggtggtttg tttgccggat caagagctac taactctttt tccgaaggta actggcttca
     2941 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3001 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3061 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3121 cgcagcggtc gggctgaacg gggggttcgt gctcacagcc cagcttggag cgaacgacct
     3181 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3241 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3301 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3361 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3421 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3481 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3541 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccacctg
     3601 caacgtcccg ctggcagaca tactgtccca caaatgaaga tggaatctgt aaaagaaaac
     3661 gcgtgaaata atgcgtctga caaaggttag gtcggctgcc tttaatcaat accaaagtgg
     3721 tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga acaaataaga
     3781 ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc ttcagact
//
