LOCUS       sequence-254701-        9210 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway compatible N2H assay destination vector with C-terminus 
            tagging of nanoluc fragment2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Choi SG, Olivet J, Cassonnet P, Vidalain PO, Luck K, Lambourne L, 
            Spirohn K, Lemmens I, Dos Santos M, Demeret C, Jones L, Rangarajan 
            S, Bian W, Coutant EP, Janin YL, van der Werf S, Trepte P, Wanker 
            EE, De Las Rivas J, Tavernier J, Twizere JC, Hao T, Hill DE, Vidal 
            M, Calderwood MA, Jacob Y
  TITLE     Maximizing binary interactome mapping with a minimal number of 
            assays.
  JOURNAL   Nat Commun. 2019 Aug 29;10(1):3907. doi: 10.1038/s41467-019-11809-2.
  PUBMED    31467278
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-29"; volume: "10(1):3907. doi"; pages: " 
            10.1038/s41467-019-11809-2"
COMMENT     Sequence Label: pDEST-N2H-C2
FEATURES             Location/Qualifiers
     source          1..9210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..44
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        65..346
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             347..1021
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1124..1579)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1261..1282)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1473..1492
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     enhancer        1716..2095
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2472..2491
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2472..2490
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2693..2817
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2842..2847,2848..2865,2866..2872)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2926..3585
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2992..3011)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3927..4232
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4111..4130
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4273..4397)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4781..4814
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5082..5203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5093..5112)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5147..5166
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      5268..5455
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5323..5347)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     rep_origin      5472..5607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5706..5723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5877..6465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5957..5976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6636..7427,7428..7496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7259..7278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7497..7601)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7628..8970
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8591..8638)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    8993..9026
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     9057..9075
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9113..9135)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
       61 gatcaacgac attactatat atataatata ggaagcattt aatagaacag catcgtaata
      121 tatgtgtact ttgcagttat gacgccagat ggcagtagtg gaagatattc tttattgaaa
      181 aatagcttgt caccttacgt acaatcttga tccggagctt ttcttttttt gccgattaag
      241 aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt ggtgactatt
      301 gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt ctgttattaa
      361 tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca cagaggccgc
      421 agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc ccaatagaaa
      481 gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa
      541 tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt
      601 tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg
      661 gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga
      721 ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga
      781 ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg
      841 aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga cgccagaaaa
      901 tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga
      961 gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata
     1021 ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgccgat ctatgcggtg
     1081 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     1141 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     1201 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     1261 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     1321 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     1381 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     1441 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     1501 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     1561 gcgccgctac agggcgcgtc aatattggcc attagccata ttattcattg gttatatagc
     1621 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat
     1681 ttatattggc tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta
     1741 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     1801 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     1861 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     1921 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc
     1981 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2041 acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     2101 gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag
     2161 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     2221 aaaatgtcgt aacaactgcg atcgggcgcg cccctccttg aattaattaa ggcgcgccac
     2281 tgaaattttt aattaaggcg cgcctagcat gtgattaatt aagcatgtct ttagatctat
     2341 gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc
     2401 ggggcggcgc ggtcccaggt ccacttcgca tataaaaggt gacgcgtgtg gatcctcgaa
     2461 caccgagctc ttaatacgac tcactatagg gaaagcaaaa atgtgatctt gcttgtaaat
     2521 acaattttga gaggttaata aattacaagt agtgctattt ttgtatttag gttagctatt
     2581 tagctttacg ttccaggatg cctagtggca gccccacaat atccaggaag ccctctctgc
     2641 ggtttttcag attaggtagt cgaaaaacct aagaaattta cctatgccca tcacaagttt
     2701 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     2761 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
     2821 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     2881 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     2941 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     3001 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     3061 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     3121 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     3181 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     3241 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     3301 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     3361 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     3421 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     3481 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     3541 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
     3601 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     3661 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     3721 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     3781 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     3841 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     3901 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     3961 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     4021 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     4081 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     4141 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     4201 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     4261 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     4321 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     4381 ttcttgtaca aagtggtgat gggtggaggg ggcagtggtg gagggggcag tggtctgagc
     4441 ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat
     4501 cactttaagg tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg
     4561 atcgactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact
     4621 gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac
     4681 ggctccctgc tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc
     4741 attctggcgt aataactata acggtcctaa ggtagcgaac ataacttcgt ataatgtatg
     4801 ctatacgaag ttatggaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4861 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4921 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5041 aaaaaaaaaa aaaaaaaaaa aaaaaaaaag agaagacatg ataagataca ttgatgagtt
     5101 tggacaaacc acaacaagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     5161 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     5221 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagcg aatttcttat
     5281 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     5341 gtgactctta ggttttaaaa cgaaaattct tgttcttgag taactctttc ctgtaggtca
     5401 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcggcc
     5461 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg cctcatggct
     5521 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5581 agtagtgagg acgctttttt ggaggccgag gcttttgcaa agatcgaaca agagacagga
     5641 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5701 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5761 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5821 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5881 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5941 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6001 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6061 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6121 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6181 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6241 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6301 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6361 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6421 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6481 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6541 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6601 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6661 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6721 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6781 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6841 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6901 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6961 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7021 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7081 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7141 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7201 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7261 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7321 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7381 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7441 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7501 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7561 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7621 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct
     7681 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc
     7741 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga
     7801 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     7861 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc
     7921 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc
     7981 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct
     8041 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg
     8101 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta
     8161 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat
     8221 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat
     8281 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt
     8341 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
     8401 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
     8461 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt
     8521 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
     8581 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa
     8641 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac
     8701 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg
     8761 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag
     8821 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc
     8881 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt
     8941 caatgctatc atttcctttg atattggatc atactaagaa accattggta ccataacttc
     9001 gtataaggta tactatacga agttatgcta gcattatcat gacattaacc tataaaaata
     9061 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     9121 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9181 cccgtcaggg cgcgtcagcg ggtgttggcg
//
