LOCUS       sequence-254839-       13242 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Dobrowolski C, Valadkhan S, Graham AC, Shukla M, Ciuffi A, Telenti 
            A, Karn J
  TITLE     Entry of Polarized Effector Cells into Quiescence Forces HIV 
            Latency.
  JOURNAL   MBio. 2019 Mar 26;10(2). pii: mBio.00337-19. doi: 
            10.1128/mBio.00337-19.
  PUBMED    30914509
REFERENCE   2  (bases 1 to 13242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-03-26"; volume: "10(2). pii: mBio.00337-19. doi"; pages:
            " 10.1128/mBio.00337-19"
COMMENT     Sequence Label: pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef
FEATURES             Location/Qualifiers
     source          1..13242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             290..923
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     CDS             complement(1213..1266)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(1276..1986,1987..1989,1990..1992))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1276..1986,1987..1989,1990..1992))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1318..1339)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1667..1686
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1926..1947
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1999..2055)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2065..2550)
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.2
                     /note="This allele of Thy1 is also known as CD90.2."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELQVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     CDS             complement(2581..2604)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(2641..2664)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(2924..2950)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(3244..3288)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3473..3706
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6067..6192
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6239..6872
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7040..7174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7064..7083)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7118..7137
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7599..7619)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7749..7814
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8948..9277)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8962..9097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9262..9282
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(9352..9369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9523..10111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9603..9622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10282..11073,11074..11142))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10905..10924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11143..11247)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11315..11333
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11371..11393)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11493..11512
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11726..11745
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        12035..12053
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     12035..12052
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aatatctgtc agaatagtaa attagataag tttatacttt aaactataaa tcattatata
       61 aatgttagta aacttaatgt aatccttagg atctgggatt agccaggtat gtgcagaatc
      121 gtgagtatct ctggtggctt cttccaagta cttctgcagc tttgcctgtg tgctccactc
      181 ctagttcaat tcctgctaaa tctattataa tgtcaaggcc tctcactctc tgatattcat
      241 ttctttgcaa gttataaata ctgaataata agatgacatg aactactact gctagagatt
      301 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
      361 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
      421 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca
      481 aaaagcagct gcttatatgc agcatctgag ggctcgccac tccccagtcc cgcccaggcc
      541 acgcctccct ggaaagtccc cagcggaaag tcccttgtag caagctcgat gtcagcagtt
      601 cttgaagtac tccggatgca gctctcgggc catgtgatga aatgctaggc ggctgtcaaa
      661 cctccactct aatacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa
      721 caagcgggtg ttctctcctt cattggcttc ttctaccttc tcttgctcaa ctggtactag
      781 cttgtagcac catccaaagg tcagtggata tctgatccct ggccctggtg tgtagttctg
      841 ccaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttcg
      901 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
      961 agctgccttg taagtcattg gtcttaaagg tacctgaggt gtgactggaa aacccacctc
     1021 ctcctcctct tgtgcttcta gccaggcaca atcagcattg gtagctgctg tattgctact
     1081 tgtgattgct ccatgttttt ctaggtctcg agatactgct cccaccccat ctgctgctgg
     1141 ctcagctcgt ctcattcttt cccttacagc aggccatcca atcacactac tttttgacca
     1201 cttgccaccc atggggccgg gattttcctc cacgtccccg catgttagaa gacttcccct
     1261 gccctctccg cttcccttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     1321 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
     1381 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     1441 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     1501 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     1561 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     1621 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     1681 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     1741 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
     1801 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
     1861 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     1921 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     1981 cttgctcacc atggttgtgg ggccggggtt ctcctccacg tcgccggcct gcttcagcag
     2041 gctgaagttg gtggcgccgc tgcccagaga aatgaagtcc agggcttgga ggagggagag
     2101 ggaaagcagc agcagcagca tccaggatgt gttctgaacc agcaggctta tgccgccaca
     2161 cttgaccagc ttgtctctat acacactgat acttttattg gagctcatgg gattcgcgcc
     2221 cgagacttga agctcacaaa agtagtcgcc ctcatccttg gtggtgaagt tggctagggt
     2281 aaggaccttg atatagggct ggttggagag ggtgacgcgg gagcggtacg tgtgctcggg
     2341 tatcccaagg gtgcctgaga gcacgtgctt cctcttctct cgggtcaggc tgaactcatg
     2401 ctggatggag ttatccttgg tgttattctc atggcggcag tccaggcgaa ggttttggtt
     2461 caccaggcag gctgtcaggc tggtcacctt ctgccctcgg gacacctgca agactgagag
     2521 caggagagcg acgctgatgg ctgggttcat agaaccacca ccaccagaac caccaccacc
     2581 tttttcgaac tgcgggtggc tccacgatcc acctcccgat ccacctccgg aacctccacc
     2641 tttctcgaac tgcgggtggc tccacatgtg gcacgcgtgt tatagcaaaa tcctttccaa
     2701 gccctgtctt attcttctag gtatgtggcg aatagctcta taagctgctt gtaatgcttc
     2761 tataacccta tctgtcccct cagctactgc tatggctgtg gcattgagca agttaacagc
     2821 actattcttt agttcctgac tccaatactg taggagattc caccaatatt tgagggcttc
     2881 ccaccccctg cgtcccagaa gttccacaat cctcgttaca atcaagagta agtctctcaa
     2941 gcggtggtag ctgaagaggc acaggctccg cagatcgtcc cagataagtg ctaaggatcc
     3001 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
     3061 gggcctgtcg ggtcccctcg ggattgggag gtgggtctga aacgataatg gtgaatatcc
     3121 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
     3181 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
     3241 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
     3301 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
     3361 ctccatccag gtcatgttat tccaaatctg ttccagagat ttattactcc aactagcatt
     3421 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
     3481 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgtt tgatgcccca
     3541 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
     3601 ctgcactata tcagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
     3661 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
     3721 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
     3781 ttttactact ttatatttat ataattcact tctccaattg tccctcatat cgcctcctcc
     3841 aggtctgaag atctcggacc cattgttgtt attaccacca tctcttgtta atagcagccc
     3901 agtaatattt gatgaacatc taatttgtcc actgatggga ggggcataca ttgcttttcc
     3961 tacttcctgc cacatgttta taaattgttt tattctgcat gggagtgtga ttgtgtcact
     4021 tccttcagtg ttatttgacc cttcagtact ccaagtacta ttaaaccaag tactattaaa
     4081 cagttgtgtt gaattacagt agaaaaattc ccctccacaa ttaaaactgt gcgttacaat
     4141 ttctgggtcc cctcctgagg attgcttaaa gattattgtt ttattatttc caaattgttc
     4201 tcttaatttg ctagctatct gttttaaagt ggcattccat tttgctctac taatgttaca
     4261 atgtgcttgt ctcatatttc ctatttttcc tattgtaaca aatgctctcc ctggtcccct
     4321 ctggatacgg atactttttc ttgtattgtt gttgggtctt gtacaattaa tttctacaga
     4381 tgtgttcagc tgtactatta tggttttagc attgtctgtg aaattggcag atctaattac
     4441 tacatcttct tctgctagac tgccatttaa cagcagttga gttgatacta ctggcctgat
     4501 tccatgtgta cattgtactg tgctgacatt tgtacatggt cctgttccat tgaacgtctt
     4561 attattacat tttagaatcg caaaaccagc cggggcacaa taatgtatgg gaattggctc
     4621 aaaggatacc tttggacagg cctgtgtaat gactgaggtg ttacaactta tcaacctata
     4681 gctggtatta tctattggta ctatatcaag tttataaaag aatgcatatt ctttctgcac
     4741 cttatctctt atgcttgtgc tgatattgaa agagcagttt tttatctctc ctttctccat
     4801 tatcattctc ccgctactac tattggtatt agtatcattc ttcaaatcag tgcactttaa
     4861 actaacacag agtggggtta attttacaca tggctttagg ctttgatccc ataaactgat
     4921 tatatcctca tgcatctgtt ctaccatgtc atttttccac atgttaaaat tttctgtcac
     4981 atttaccaat actacttctt gtgggttggg gtctgtgggt acacaggcat gtgtggccca
     5041 aacattatgt acctctgtat catatgcttt agcatctgat gcacaaaata gagtggtggt
     5101 tgcttccttc cacacaggta ccccataata gactgtgacc cacaattttt ctgtagcact
     5161 acagatcatc aatatcccaa ggagcatggt gccccatttc cacccccatc tccacaagtg
     5221 ctgatacttc tccttcactc tcattgccac tgtcttctgc tctttctatt agtctatcaa
     5281 ttaacctgtc tatttttctt tgtcttaata ttttcctata ttctatgatt actatggacc
     5341 acacaactat tgctattatt attgctacta ctaatgctac tattgctact attataggtt
     5401 gcattacatg tactacttac tgctttgata gagaagcttg atgagtctga ctgttctgat
     5461 gagctcttcg tcgctgtctc cgcttcttcc tgccatagga gatgcctaag gcttttgtca
     5521 tgaaacaaac ttggcaatga aagcaacact ttttacaata gcaattggta caagcagttt
     5581 taggctgact tcctggatgc ttccagggct ctagtctagg atctactggc tccatttctt
     5641 gctctcctct gtcgagtaac gcctattctg ctatgtcgac acccaattct gaaatggata
     5701 aacagcagtt gttgcagaat tcgcggccgc ttgctgtgcg gtggtcttac ttttgttttg
     5761 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
     5821 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
     5881 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg
     5941 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
     6001 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
     6061 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
     6121 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
     6181 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
     6241 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     6301 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     6361 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     6421 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
     6481 gcccaggcca cgcctccctg gaaagtcccc agcggaaagt cccttgtagc aagctcgata
     6541 tcagcagttc ttgaagtact ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg
     6601 gctgtcaaac ctccactcta acacttctct ctccgggtca tccatcccat gcaggctcac
     6661 agggtgtaac aagctggtgt tctctccttt attggcctct tctaccttat ctggctcaac
     6721 tggtactagc ttgtagcacc atccaaaggt cagtggatat ctgacccctg gccctggtgt
     6781 gtagttctgc taatcaggga agtagccttg tgtgtggtag atccacagat caaggatatc
     6841 ttgtcttctt tgggagtgaa ttagcccttc caactactaa gtttgtagta catatttaac
     6901 aaatacaatt tctttaaaat gaaaataatt cagaggaatc acaggtttag agtaaatgaa
     6961 accacaggta attggcagtg gtaatagggt atggggtggg aagtttggga tgattttggt
     7021 tagcttgagt tatccagttg atccagacat gataagatac attgatgagt ttggacaaac
     7081 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     7141 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     7201 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     7261 tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc cttattaacc
     7321 cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt aatagcagac
     7381 actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact gttatgccta
     7441 cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc agctttttcc
     7501 tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa
     7561 aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga
     7621 ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat ttcttctgag
     7681 caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc agttccatag
     7741 gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta tacacttaaa
     7801 aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca
     7861 ccacagaagt aaggttcctt cacaaagatc cggggcccac tcataaatcc agttgccgcc
     7921 acggtagcca atcaccgtat cgtataaatc atcgtcggta cgttcggcat cgctcatcac
     7981 aatacgtgcc tggacgtcga ggatttcgcg tgggtcaatg ccgcgccaga tccacatcag
     8041 acggttaatc atgcgatacc agtgagggat ggttttacca tcaagggccg actgcacagg
     8101 cggttgtgcg ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg
     8161 gggtttgaac agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact
     8221 tactggaaac tattgtaacc cgcctgaagt taaaaagaac aacgcccggc agtgccaggc
     8281 gttgaaaaga ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct
     8341 gttcaatcca ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag
     8401 ccggttttgc gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaaccg
     8461 cagtaccacc ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg
     8521 cgcgtttcag cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg
     8581 tatcgacatg acgaataccc agttcacgcg ccagtaacgc acccggtaca gaccgccacg
     8641 gcttacggca ataatgcctt tccattgttc agaaggcatc agtcggcttg cgagtttacg
     8701 tgcatggatc tgcaacatgt cccaggtgac gatgtatttt tcgctcatgt gaagtgtccc
     8761 agcctgttta tctacggctt aaaaagtgtt cgaggggaaa ataggttgcg cgagattata
     8821 gagatctggc gcactaaaaa ccagtatttc acatgagtcc gcgtcttttt acgcactgcc
     8881 tctccctgac gcgggataaa gtggtattct caaacatatc tcgcaagcct gtcttgtgtc
     8941 caagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     9001 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     9061 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     9121 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     9181 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     9241 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgc attaatgaat
     9301 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10441 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10501 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10561 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10621 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10681 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10741 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10801 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10861 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10921 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10981 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11041 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11101 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11161 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11221 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11281 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11341 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11401 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11461 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11521 gcaccattcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
    11581 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
    11641 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
    11701 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
    11761 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg ctagcgatga
    11821 ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca gaaactcaga
    11881 aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat agggtctgct
    11941 tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa cgcggctaca
    12001 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaatacacg
    12061 gaattaattc tagattactt ataataccta atagattgta aatgctaaat aaagttgtta
    12121 tactgtgctg tttttctgat ttgtattttt ttattgttgt attgctattt ttaatttttt
    12181 cccaatattt ttgatccaca gttggttgga tttttgttgt ggaacctgca gatgaggaag
    12241 gctgacttta tatgataata tcaaactgga aaaattgatg ttagaataaa gatgtgtttc
    12301 tgagattaaa ttagatttta ataaaaatta ccccagtctc aggttttcta tctgcaatgt
    12361 gaaatgatct cagagaagaa taaattttaa ccatttacat tattaaattg aaatcactaa
    12421 aagtaaaaca aaggtgtgag actatataat agattgtttt ccttatttca gagtctttgg
    12481 aacaaattct tccaagataa agaacttcga tcaatgattg aacaagatgt caaaagaacg
    12541 tatgtagaac tatttacatg ttactttcaa taattcaaat tgttgttatc tctttctaat
    12601 tttaacaaaa cacaaaatct gcagtcaact taattgaatg cagtattcta cttgtgttaa
    12661 taacaaactt gttttgacaa attgtgataa taaggattgt tttctactct aatgatacac
    12721 atttctatgt tcccttttta aaagtaaatt acatttataa tattgataat cttcaataat
    12781 atttaattag ttttatcaag ttccaacaat tatttgaagt catatttaag gttatattgt
    12841 atagtcttgt tgccagaaaa atttatccct tgctttatgc atttgaatcc ctttctaacc
    12901 aagttacttg ttaaaacttg ttaaatgaca actgttattc ttatcctcac ttctcttctt
    12961 cttgaagcta ttcaggtttg caaacaattt cttctctcaa tctactcagt atttactata
    13021 taaaagggta atttgttact tagcataatg tccataactt tgatatattc gactgagtca
    13081 tagggtgagg agtaaatgga atttgtgtca gaagatcata gagtcgtaac taacggatgc
    13141 tgtaattgtg ggaaaattac ttcaacatct ttccctctca gtttcctctt gtgttaaatg
    13201 gggataatag taatcatctc ttatgatatt tgaggattaa tt
//
