LOCUS       sequence-255014-        9864 bp    DNA     circular SYN 14-APR-2025
DEFINITION  pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/
            N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Franklin B, Koob J, Kellner MJ, Ladha 
            A, Joung J, Kirchgatterer P, Cox DBT, Zhang F
  TITLE     A cytosine deaminase for programmable single-base RNA editing.
  JOURNAL   Science. 2019 Jul 11. pii: science.aax7063. doi: 
            10.1126/science.aax7063.
  PUBMED    31296651
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Jul 11. pii: science.aax7063. doi: 10.1126/science.aax7063."
COMMENT     Sequence Label: pC0078 RESCUE
FEATURES             Location/Qualifiers
     source          1..9864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      683..692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5221..5238)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5227..5451
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5497..5925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5584..5603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5794..5815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5934..5954)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5939..6268
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6119..6254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6181..6200
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6335..7129
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6389..6408)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6999..7018
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7303..7424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7340..7359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7394..7413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7473..7489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7473..7489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7486..7508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7497..7513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7521..7527,7528..7545,7546..7551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7566..7587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7704..7721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7875..8463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7955..7974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8634..9425,9426..9494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9257..9276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9495..9599)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9674..9693)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttgccaccat ggagaagccc ctgctgccta acgtgtatac
      721 cctgaagcac aagttctttt ggggcgcctt cctgaatatc gcccggcaca acgcctttat
      781 caccatctgc cacatcaatg agcagctggg cctgaaaacc cccagcaacg acgataagat
      841 cgtggacgtg gtgtgcgaga catggaacaa tatcctgaac aatgaccacg atctgctgaa
      901 gaagtcccag ctgaccgagc tgatcctgaa gcacttccct tttctgacag ccatgtgcta
      961 ccacccccct aagaaggagg gcaagaagaa gggccaccag aaggagcagc agaaggagaa
     1021 ggagagcgag gcacagtccc aggcagaggc cctgaaccca tccaagctga tcgaggccct
     1081 ggagatcctg gtgaatcagc tgcactctct ggcgaactac tatagcgcct ataagcacaa
     1141 gaagcccgac gccgagaagg atatcttcaa gcacctgtat aaggccttcg acgcctctct
     1201 gagaatggtg aaggaggatt ataaggccca cttcaccgtg aatctgacaa gggactttgc
     1261 ccacctgaac cgcaagggca agaacaagca ggacaatccc gacttcaaca gatacagatt
     1321 cgagaaggat ggcttcttta ccgagtctgg cctgctgttc tttacaaatc tgtttctgga
     1381 caagagggat gcctattgga tgctgaagaa ggtgtccggc ttcaaggcct ctcacaagca
     1441 gcgcgagaag atgaccacag aggtgttttg caggagccgc atcctgctgc ccaagctgag
     1501 gctggagtcc cgctacgacc acaaccagat gctgctggat atgctgtctg agctgagcag
     1561 atgtcctaag ctgctgtatg agaagctgag cgaggagaat aagaagcact tccaggtgga
     1621 ggccgacggc tttctggatg agatcgagga ggagcagaac ccattcaagg acaccctgat
     1681 ccggcaccag gatagattcc cctactttgc cctgaggtat ctggacctga atgagtcctt
     1741 caagtctatc cgctttcagg tggatctggg cacataccac tattgtatct acgacaagaa
     1801 gatcggcgat gagcaggaga agaggcacct gacccgcaca ctgctgtcct tcggccggct
     1861 gcaggacttt accgagatca acagacccca ggagtggaag gccctgacca aggacctgga
     1921 ttacaaggag acatccaatc agcctttcat ctctaagacc acaccacact atcacatcac
     1981 cgacaacaag atcggctttc ggctgggcac aagcaaggag ctgtacccct ccctggagat
     2041 caaggatggc gccaatagaa tcgccaagta cccttataac tccggcttcg tggcccacgc
     2101 ctttatctct gtgcacgagc tgctgcctct gatgttctac cagcacctga ccggcaagag
     2161 cgaggacctg ctgaaggaga cagtgcggca catccagaga atctataagg acttcgagga
     2221 ggagcggatc aataccatcg aggatctgga gaaggcaaac cagggcagac tgccactggg
     2281 agcctttccc aagcagatgc tgggcctgct gcagaataag cagcctgatc tgtccgagaa
     2341 ggccaagatc aagatcgaga agctgatcgc cgagacaaag ctgctgtctc acaggctgaa
     2401 cacaaagctg aagagctccc caaagctggg caagcggaga gagaagctga tcaagacagg
     2461 cgtgctggcc gactggctgg tgaaggattt catgcgcttt cagcccgtgg cctacgacgc
     2521 ccagaatcag cctatcaagt ctagcaaggc caactccacc gagttctggt ttatcaggcg
     2581 cgccctggcc ctgtacggag gagagaagaa taggctggag ggctatttca agcagaccaa
     2641 cctgatcggc aacacaaatc cacacccctt cctgaacaag tttaattgga aggcctgcag
     2701 gaatctggtg gatttctacc agcagtatct ggagcagcgc gagaagtttc tggaggccat
     2761 caagaaccag ccatgggagc cctaccagta ttgcctgctg ctgaagatcc ctaaggagaa
     2821 caggaagaat ctggtgaagg gatgggagca gggaggaatc tctctgccac ggggcctgtt
     2881 taccgaggcc atcagagaga cactgtctga ggacctgatg ctgagcaagc caatccgcaa
     2941 ggagatcaag aagcacggcc gggtgggctt catcagcaga gccatcaccc tgtactttaa
     3001 ggagaagtat caggataagc accagagctt ctacaatctg tcctataagc tggaggcaaa
     3061 ggcaccactg ctgaagcggg aggagcacta cgagtattgg cagcagaaca agcctcagtc
     3121 tccaaccgag agccagaggc tggagctgca cacaagcgac cgctggaagg attacctgct
     3181 gtataagaga tggcagcacc tggagaagaa gctgcggctg taccggaatc aggacgtgat
     3241 gctgtggctg atgaccctgg agctgacaaa gaaccacttc aaggagctga acctgaatta
     3301 tcaccagctg aagctggaga atctggccgt gaacgtgcag gaggccgatg ccaagctgaa
     3361 ccctctgaat cagaccctgc ccatggtgct gcctgtgaag gtgtaccctg ccacagcctt
     3421 tggcgaggtg cagtaccaca agaccccaat caggacagtg tatatccgcg aggagcacac
     3481 caaggccctg aagatgggca acttcaaggc cctggtgaag gaccggagac tgaatggcct
     3541 gttctctttt atcaaggagg agaacgatac acagaagcac ccaatcagcc agctgcggct
     3601 gaggcgcgag ctggagatct accagtctct gagagtggac gccttcaagg agacactgag
     3661 cctggaggag aagctgctga ataagcacac atccctgtcc tctctggaga acgagtttag
     3721 ggccctgctg gaggagtgga agaaggagta tgccgccagc tccatggtga cagacgagca
     3781 catcgccttc atcgccagcg tggccaatgc cttctgcgcc aaccagtacc ccttctacaa
     3841 ggaggccctg cacgccccta tcccactgtt caccgtggcc cagcccacca cagaggagaa
     3901 ggatggcctg ggaatcgcag aggccctgct gaaggtgctg agggagtact gtgagatcgt
     3961 gaagtcccag atcggatcca gcctccaaaa aaagttggag gaattagaac taggatccca
     4021 gctgcattta ccgcaggttt tagctgacgc tgtctcacgc ctggtcatag gtaagtttgg
     4081 tgacctgacc gacaacttct cctcccctca cgctcgcaga ataggtctgg ctggagtcgt
     4141 catgacaaca ggcacagatg ttaaagatgc caaggtgata tgtgtttcta caggatctaa
     4201 atgtattaat ggtgaatacc taagtgatcg tggccttgca ttaaatgact gccatgcaga
     4261 aatagtatct cggagatcct tgctcagatt tctttataca caacttgagc tttacttaaa
     4321 taacgaggat gatcaaaaaa gatccatctt tcagaaatca gagcgagggg ggtttaggct
     4381 gaaggagaat atacagtttc atctgtacat cagcacctct ccctgtggag atgccagaat
     4441 cttctcacca catgaggcaa tcctggaaga accagcagat agacacccaa atcgtaaagc
     4501 aagaggacag ctacggacca aaatagaggc tggtcagggg acgattccag tgcgcaacaa
     4561 tgcgagcatc caaacgtggg acggggtgct gcaaggggag cggctgctca ccatgtcctg
     4621 cagtgacaag attgcacgct ggaacgtggt gggcatccag ggatcactgc tcagcatttt
     4681 cgtggagccc atttacttct cgagcatcat cctgggcagc ctttaccacg gggaccacct
     4741 ttccagggcc atgtaccagc ggatctccaa catagaggac ctgccacctc tctacaccct
     4801 caacaagcct ttgctcacag gcatcagcaa tgcagaagca cggcagccag ggaaggcccc
     4861 catattcagt gtcaactgga cggtaggcga ctccgctatt gaggtcatca acgccacgac
     4921 tgggaaggga gagctgggcc gcgcgtcccg cctgtgtaag cacgcgttgt actgtcgctg
     4981 gatgcgtgtg cacggcaagg ttccctccca cttactacgc tccaagatta ccaagcccaa
     5041 cgtgtaccat gagacaaagc tggcggcaaa ggagtaccag gccgccaagg cgcgtctgtt
     5101 cacagccttc atcaaggcgg ggctgggggc ctgggtggag aagcccaccg agcaggacca
     5161 gttctcactc acgtaagcgg ccgctcgagt ctagagggcc catttaaacc cgctgatcag
     5221 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5281 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5341 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5401 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5461 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5521 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5581 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5641 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5701 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5761 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5821 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5881 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     5941 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6001 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6061 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6121 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6181 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6241 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6301 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     6361 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6421 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6481 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     6541 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6601 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6661 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6721 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6781 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6841 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6901 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6961 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7021 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     7081 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     7141 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     7201 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     7261 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     7321 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7381 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     7441 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7501 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7561 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7621 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7681 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7741 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7801 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7861 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7921 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7981 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8041 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8101 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8161 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8221 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8281 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8341 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8401 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8461 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8521 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8581 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8641 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8701 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8761 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8821 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8881 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8941 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9001 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9061 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9121 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9181 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9241 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9301 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9361 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9421 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9481 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9541 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9601 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     9661 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     9721 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     9781 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     9841 atgtacgggc cagatatacg cgtt
//
