LOCUS       sequence-255133-       11943 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses Thy1.1 and FOXO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11943)
  TITLE     Foxo-Thy1 Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMJJ4-FOXO1-IRES-Thy1.1
FEATURES             Location/Qualifiers
     source          1..11943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2040..2343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2344..2547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2498..2518
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2544..2568
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2653..2662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4674..5240
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4690..5242
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4834..4851)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5061..5080
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5290..5778
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    5806..6394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5859..5879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6277..6288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6397..6413)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6398..6414)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6504..6737
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6762..6780)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6763..6780)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7070..7089)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7303..7322)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7422..7444
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7482..7500)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7568..7672
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7673..7741,7742..8533)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7891..7910)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8704..9292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9193..9212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9446..9463
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9533..9553)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9538..9867
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9718..9853
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11001..11066
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11197..11217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11642..11776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11679..11698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11733..11752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgaaggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gataagcttg ggagttccgc
     2041 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2101 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2161 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2221 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2281 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2341 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2401 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2461 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2521 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     2581 catccacgct gttttgacct ccatagaaga caccgactct agctagagga tcatttaaat
     2641 ttaattaagt ttgccaccat ggccgaggcg cctcaggtgg tggagatcga cccggacttc
     2701 gagccgctgc cccggccgcg ctcgtgcacc tggccgctgc ccaggccgga gtttagccag
     2761 tccaactcgg ccacctccag cccggcgccg tcgggcagcg cggctgccaa ccccgacgcc
     2821 gcggcgggcc tgccctcggc ctcggctgcc gctgtcagcg ccgacttcat gagcaacctg
     2881 agcttgctgg aggagagcga ggacttcccg caggcgcccg gctccgtggc ggcggcggtg
     2941 gcggcggcgg ccgccgcggc cgccaccggg gggctgtgcg gggacttcca gggcccggag
     3001 gcgggctgcc tgcacccagc gccaccgcag cccccgccgc ccgggccgct gtcgcagcac
     3061 ccgccggtgc cccccgccgc cgctgggccg ctcgcggggc agccgcgcaa gagcagctcg
     3121 tcccgccgca acgcgtgggg caacctgtcc tacgccgacc tcatcaccaa ggccatcgag
     3181 agctcggcgg agaagcggct cacgctgtcg cagatctacg agtggatggt caagagcgtg
     3241 ccctacttca aggataaggg tgacagcaac agctcggcgg gctggaagaa ttcaattcgt
     3301 cataatctgt ccctacacag caagttcatt cgtgtgcaga atgaaggaac tggaaaaagt
     3361 tcttggtgga tgctcaatcc agagggtggc aagagcggga aatctcctag gagaagagct
     3421 gcatccatgg acaacaacag taaatttgct aagagccgaa gccgagctgc caagaagaaa
     3481 gcatctctcc agtctggcca ggagggtgct ggggacagcc ctggatcaca gttttccaaa
     3541 tggcctgcaa gccctggctc tcacagcaat gatgactttg ataactggag tacatttcgc
     3601 cctcgaacta gctcaaatgc tagtactatt agtgggagac tctcacccat tatgaccgaa
     3661 caggatgatc ttggagaagg ggatgtgcat tctatggtgt acccgccatc tgccgcaaag
     3721 atggcctcta ctttacccag tctgtctgag ataagcaatc ccgaaaacat ggaaaatctt
     3781 ttggataatc tcaaccttct ctcatcacca acatcattaa ctgtttcgac ccagtcctca
     3841 cctggcacca tgatgcagca gacgccgtgc tactcgtttg cgccaccaaa caccagtttg
     3901 aattcaccca gcccaaacta ccaaaaatat acatatggcc aatccagcat gagccctttg
     3961 ccccagatgc ctatacaaac acttcaggac aataagtcga gttatggagg tatgagtcag
     4021 tataactgtg cgcctggact cttgaaggag ttgctgactt ctgactctcc tccccataat
     4081 gacattatga caccagttga tcctggggta gcccagccca acagccgggt tctgggccag
     4141 aacgtcatga tgggccctaa ttcggtcatg tcaacctatg gcagccaggc atctcataac
     4201 aaaatgatga atcccagctc ccatacccac cctggacatg ctcagcagac atctgcagtt
     4261 aacgggcgtc ccctgcccca cacggtaagc accatgcccc acacctcggg tatgaaccgc
     4321 ctgacccaag tgaagacacc tgtacaagtg cctctgcccc accccatgca gatgagtgcc
     4381 ctggggggct actcctccgt gagcagctgc aatggctatg gcagaatggg ccttctccac
     4441 caggagaagc tcccaagtga cttggatggc atgttcattg agcgcttaga ctgtgacatg
     4501 gaatccatca ttcggaatga cctcatggat ggagatacat tggattttaa ctttgacaat
     4561 gtgttgccca accaaagctt cccacacagt gtcaagacaa cgacacatag ctgggtgtca
     4621 ggctaaggat ccaaacgatc ccctcgacgg tatcgataag cttgatatcg aattccgccc
     4681 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4741 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4801 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4861 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     4921 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     4981 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5041 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5101 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     5161 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5221 tttgaaaaac acgatgataa tatggccaca accatgggtc ttttctgcag tcaccgtcct
     5281 cgaggcacca tgaacccagc catcagcgtc gctctcctgc tctcagtctt gcaggtgtcc
     5341 cgagggcaga aggtgaccag cctgacagcc tgcctggtga accaaaacct tcgcctggac
     5401 tgccgccatg agaataacac caaggataac tccatccagc atgagttcag cctgacccga
     5461 gagaagagga agcacgtgct ctcaggcacc cttgggatac ccgagcacac gtaccgctcc
     5521 cgcgtcaccc tctccaacca gccctatatc aaggtcctta ccctagccaa cttcaccacc
     5581 aaggatgagg gcgactactt ttgtgagctt cgcgtctcgg gcgcgaatcc catgagctcc
     5641 aataaaagta tcagtgtgta tagagacaag ctggtcaagt gtggcggcat aagcctgctg
     5701 gttcagaaca catcctggat gctgctgctg ctgctttccc tctccctcct ccaagccctg
     5761 gacttcattt ctctgtgacg cgaattcgat atcaagctta tcgataatca acctctggat
     5821 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5881 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5941 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6001 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6061 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6121 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6181 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6241 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6301 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6361 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     6421 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6481 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6541 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6601 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6661 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6721 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     6781 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     6841 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     6901 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     6961 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     7021 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     7081 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     7141 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     7201 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     7261 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     7321 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7381 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     7441 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     7501 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     7561 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7621 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7681 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7741 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7801 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7861 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7921 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7981 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8041 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8101 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8161 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8221 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8281 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8341 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8401 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8461 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8521 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8581 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8821 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8881 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8941 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9001 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9061 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9121 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9181 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9241 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9301 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9361 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9421 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9481 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     9541 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9601 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9661 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9721 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9781 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9841 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     9901 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     9961 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
    10021 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
    10081 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
    10141 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
    10201 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
    10261 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
    10321 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
    10381 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
    10441 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
    10501 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
    10561 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
    10621 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
    10681 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
    10741 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
    10801 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
    10861 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
    10921 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
    10981 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
    11041 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
    11101 ggtggaatgc ctttaatgag gtaaacctgt tttgctcaga agaaaatgcc atctagtgat
    11161 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    11221 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    11281 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    11341 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    11401 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    11461 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    11521 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    11581 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    11641 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11761 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11821 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa ccctgtgatt
    11881 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    11941 tag
//
