LOCUS       sequence-255164-       11997 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses Thy1.1 and FOXO3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11997)
  TITLE     Foxo-Thy1 Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMJJ4-FOXO3-IRES-Thy1.1
FEATURES             Location/Qualifiers
     source          1..11997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2040..2343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2344..2547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2498..2518
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2544..2568
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2653..2662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4728..5294
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4744..5296
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4888..4905)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5115..5134
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5344..5832
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    5860..6448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5913..5933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6331..6342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6451..6467)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6452..6468)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6558..6791
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6816..6834)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6817..6834)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7124..7143)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7357..7376)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7476..7498
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7536..7554)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7622..7726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7727..7795,7796..8587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7945..7964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8758..9346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9247..9266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9500..9517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9587..9607)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9592..9921
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9772..9907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11055..11120
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11251..11271
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11696..11830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11733..11752)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11787..11806
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgaaggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gataagcttg ggagttccgc
     2041 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2101 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2161 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2221 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2281 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2341 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2401 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2461 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2521 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     2581 catccacgct gttttgacct ccatagaaga caccgactct agctagagga tcatttaaat
     2641 ttaattaagt ttgccaccat ggcagaggca ccggcttccc cggccccgct ctctccgctc
     2701 gaagtggagc tggacccgga gttcgagccc cagagccgtc cgcgatcctg tacgtggccc
     2761 ctgcaaaggc cggagctcca agcgagccct gccaagccct cgggggagac ggccgctgac
     2821 tccatgatcc ccgaggagga ggacgatgaa gacgacgagg acggcggggg acgggccggc
     2881 tcggccatgg cgatcggcgg cggcggcggg agcggcacgc tgggctccgg gctgctcctt
     2941 gaggactcgg cccgggtgct ggcacccgga gggcaagacc ccgggtctgg gccagccacc
     3001 gcggcgggcg ggctgagcgg gggtacacag gcgctgctgc agcctcagca accgctgcca
     3061 ccgccgcagc cgggggcggc tgggggctcc gggcagccga ggaaatgttc gtcgcggcgg
     3121 aacgcctggg gaaacctgtc ctacgcggac ctgatcaccc gcgccatcga gagctccccg
     3181 gacaaacggc tcactctgtc ccagatctac gagtggatgg tgcgttgcgt gccctacttc
     3241 aaggataagg gcgacagcaa cagctctgcc ggctggaaga actccatccg gcacaacctg
     3301 tcactgcata gtcgattcat gcgggtccag aatgagggaa ctggcaagag ctcttggtgg
     3361 atcatcaacc ctgatggggg gaagagcgga aaagcccccc ggcggcgggc tgtctccatg
     3421 gacaatagca acaagtatac caagagccgt ggccgcgcag ccaagaagaa ggcagccctg
     3481 cagacagccc ccgaatcagc tgacgacagt ccctcccagc tctccaagtg gcctggcagc
     3541 cccacgtcac gcagcagtga tgagctggat gcgtggacgg acttccgttc acgcaccaat
     3601 tctaacgcca gcacagtcag tggccgcctg tcgcccatca tggcaagcac agagttggat
     3661 gaagtccagg acgatgatgc gcctctctcg cccatgctct acagcagctc agccagcctg
     3721 tcaccttcag taagcaagcc gtgcacggtg gaactgccac ggctgactga tatggcaggc
     3781 accatgaatc tgaatgatgg gctgactgaa aacctcatgg acgacctgct ggataacatc
     3841 acgctcccgc catcccagcc atcgcccact gggggactca tgcagcggag ctctagcttc
     3901 ccgtatacca ccaagggctc gggcctgggc tccccaacca gctcctttaa cagcacggtg
     3961 ttcggacctt catctctgaa ctccctacgc cagtctccca tgcagaccat ccaagagaac
     4021 aagccagcta ccttctcttc catgtcacac tatggtaacc agacactcca ggacctgctc
     4081 acttcggact cacttagcca cagcgatgtc atgatgacac agtcggaccc cttgatgtct
     4141 caggccagca ccgctgtgtc tgcccagaat tcccgccgga acgtgatgct tcgcaatgat
     4201 ccgatgatgt cctttgctgc ccagcctaac cagggaagtt tggtcaatca gaacttgctc
     4261 caccaccagc accaaaccca gggcgctctt ggtggcagcc gtgccttgtc gaattctgtc
     4321 agcaacatgg gcttgagtga gtccagcagc cttgggtcag ccaaacacca gcagcagtct
     4381 cctgtcagcc agtctatgca aaccctctcg gactctctct caggctcctc cttgtactca
     4441 actagtgcaa acctgcccgt catgggccat gagaagttcc ccagcgactt ggacctggac
     4501 atgttcaatg ggagcttgga atgtgacatg gagtccatta tccgtagtga actcatggat
     4561 gctgatgggt tggattttaa ctttgattcc ctcatctcca cacagaatgt tgttggtttg
     4621 aacgtgggga acttcactgg tgctaagcag gcctcatctc agagctgggt gccaggctaa
     4681 ggatccaaac gatcccctcg acggtatcga taagcttgat atcgaattcc gccccccccc
     4741 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     4801 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     4861 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     4921 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     4981 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5041 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5101 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5161 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     5221 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5281 aaacacgatg ataatatggc cacaaccatg ggtcttttct gcagtcaccg tcctcgaggc
     5341 accatgaacc cagccatcag cgtcgctctc ctgctctcag tcttgcaggt gtcccgaggg
     5401 cagaaggtga ccagcctgac agcctgcctg gtgaaccaaa accttcgcct ggactgccgc
     5461 catgagaata acaccaagga taactccatc cagcatgagt tcagcctgac ccgagagaag
     5521 aggaagcacg tgctctcagg cacccttggg atacccgagc acacgtaccg ctcccgcgtc
     5581 accctctcca accagcccta tatcaaggtc cttaccctag ccaacttcac caccaaggat
     5641 gagggcgact acttttgtga gcttcgcgtc tcgggcgcga atcccatgag ctccaataaa
     5701 agtatcagtg tgtatagaga caagctggtc aagtgtggcg gcataagcct gctggttcag
     5761 aacacatcct ggatgctgct gctgctgctt tccctctccc tcctccaagc cctggacttc
     5821 atttctctgt gacgcgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     5881 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5941 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6001 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6061 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6121 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6181 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6241 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     6301 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6361 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6421 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc
     6481 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6541 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6601 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6781 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     6841 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     6901 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     6961 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     7021 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     7081 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     7141 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     7201 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     7261 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     7321 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     7381 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7441 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7501 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7561 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7621 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7681 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7741 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7801 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7861 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7921 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7981 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8041 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8101 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8161 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8221 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8281 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8341 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8401 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8461 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8521 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8581 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8641 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8701 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8761 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8881 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9361 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9421 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9481 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9541 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     9601 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9661 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9721 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9901 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     9961 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
    10021 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
    10081 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
    10141 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
    10201 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
    10261 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
    10321 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
    10381 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
    10441 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    10501 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    10561 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    10621 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    10681 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    10741 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    10801 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    10861 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    10921 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    10981 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    11041 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    11101 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    11161 atgcctttaa tgaggtaaac ctgttttgct cagaagaaaa tgccatctag tgatgatgag
    11221 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
    11281 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
    11341 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
    11401 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
    11461 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
    11521 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
    11581 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    11641 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    11701 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    11761 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    11821 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    11881 ccctattacc actgccaatt acctgtggtt tcatttactc taaaccctgt gattcctctg
    11941 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtag
//
