LOCUS       sequence-255487-        7334 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Vector encoding PPARD cDNA for retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Legrand N, Bretscher CL, Zielke S, Wilke B, Daude M, Fritz B, 
            Diederich WE, Adhikary T
  TITLE     PPARbeta/delta recruits NCOR and regulates transcription 
            reinitiation of ANGPTL4.
  JOURNAL   Nucleic Acids Res. 2019 Oct 10;47(18):9573-9591. doi: 
            10.1093/nar/gkz685.
  PUBMED    31428774
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-10-10- 10"; volume: "47"; issue: 
            "18"; pages: "9573-9591"
COMMENT     Sequence Label: pMSCVbsd-PPARD-K422A
FEATURES             Location/Qualifiers
     source          1..7334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2955..3454
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2990..3013)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3387..3406
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3475..3873
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    4405..4421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4429..4435,4436..4453,4454..4459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4474..4495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4612..4629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4783..5371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4863..4882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5542..6333,6334..6402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6165..6184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6403..6507)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6575..6593
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6631..6653)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6753..6772
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6966..6988
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7116..7135
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatccact agtaacggcc gccagtgtgc
     1441 tggaattcgc ccttaggtgt ggcgccgagg ggagatcagc catggagcag ccacaggagg
     1501 aagcccctga ggtccgggaa gaggaggaga aagaggaagt ggcagaggca gaaggagccc
     1561 cagagctcaa tgggggacca cagcatgcac ttccttccag cagctacaca gacctctccc
     1621 ggagctcctc gccaccctca ctgctggacc aactgcagat gggctgtgac ggggcctcat
     1681 gcggcagcct caacatggag tgccgggtgt gcggggacaa ggcatcgggc ttccactacg
     1741 gtgttcatgc atgtgagggg tgcaagggct tcttccgtcg tacgatccgc atgaagctgg
     1801 agtacgagaa gtgtgagcgc agctgcaaga ttcagaagaa gaaccgcaac aagtgccagt
     1861 actgccgctt ccagaagtgc ctggcactgg gcatgtcaca caacgctatc cgttttggtc
     1921 ggatgccgga ggctgagaag aggaagctgg tggcagggct gactgcaaac gaggggagcc
     1981 agtacaaccc acaggtggcc gacctgaagg ccttctccaa gcacatctac aatgcctacc
     2041 tgaaaaactt caacatgacc aaaaagaagg cccgcagcat cctcaccggc aaagccagcc
     2101 acacggcgcc ctttgtgatc cacgacatcg agacattgtg gcaggcagag aaggggctgg
     2161 tgtggaagca gttggtgaat ggcctgcctc cctacaagga gatcagcgtg cacgtcttct
     2221 accgctgcca gtgcaccaca gtggagaccg tgcgggagct cactgagttc gccaagagca
     2281 tccccagctt cagcagcctc ttcctcaacg accaggttac ccttctcaag tatggcgtgc
     2341 acgaggccat cttcgccatg ctggcctcta tcgtcaacaa ggacgggctg ctggtagcca
     2401 acggcagtgg ctttgtcacc cgtgagttcc tgcgcagcct ccgcaaaccc ttcagtgata
     2461 tcattgagcc taagtttgaa tttgctgtca agttcaacgc cctggaactt gatgacagtg
     2521 acctggccct attcattgcg gccatcattc tgtgtggaga ccggccaggc ctcatgaacg
     2581 ttccacgggt ggaggctatc caggacacca tcctgcgtgc cctcgaattc cacctgcagg
     2641 ccaaccaccc tgatgcccag tacctcttcc ccaagctgct gcagaagatg gctgacctgc
     2701 ggcaactggt caccgagcac gcccagatga tgcagcggat caaggccacc gaaaccgaga
     2761 cctcgctgca ccctctgctc caggagatct acaaggacat gtactaacgg cggcacccag
     2821 gcctccctgc agactccaat ggggccagca ctggaggggc ccacccacat gacttttcca
     2881 ttgaccagct ctcaagggcg aattctgcag atatccatca cactggcggc cgctcgaggt
     2941 taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     3001 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     3061 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     3121 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     3181 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     3241 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     3301 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     3361 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     3421 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatggcc
     3481 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
     3541 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
     3601 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
     3661 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
     3721 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
     3781 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
     3841 ttgctgccct ctggttatgt gtgggagggc taatcgataa aataaaagat tttatttagt
     3901 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agagaaccat
     3961 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     4021 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     4081 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg
     4141 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     4201 tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga
     4261 agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag
     4321 ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag
     4381 aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4441 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4501 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4561 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4621 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4681 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5341 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5401 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5461 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5521 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5581 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5641 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5701 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5761 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5821 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5881 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5941 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6001 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6061 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6121 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6181 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6241 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6301 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6361 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6421 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6481 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6541 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6601 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6661 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6721 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     6781 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     6841 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6901 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     6961 gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg
     7021 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     7081 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     7141 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta
     7201 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat
     7261 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
     7321 cagaaaaagg gggg
//
