LOCUS       sequence-255503-       11476 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: MLKG1delKASH
FEATURES             Location/Qualifiers
     source          1..11476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2019..2043)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2415..2594
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2630..3655
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3853..4105
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4198..4222
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4743..5534
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5780..6368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6269..6288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6522..6539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6554..6694
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6620..6639)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6780..6802
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7038..7232
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7298..8371)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8800..9429)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10760..10784
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccaatcctcg tcgagctgga cggggatgtt
      541 aacggccaca agttctccgt gagcggcgag ggggagggcg acgccaccta cgggaagctc
      601 acgctgaagt tcatttgcac gaccggcaag ctgccggtgc cctggccaac cctggtcaca
      661 actctgacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaaga gcgccatgcc ggagggctac gttcaggagc gcaccatctt cttcaaggac
      781 gatggcaact acaagacaag ggcggaggtt aagttcgagg gcgacactct cgtgaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata ttctcggcca caagctggag
      901 tacaactaca attcgcataa cgtctacatc atggctgaca agcagaagaa cggcattaag
      961 gttaatttca agatccgcca caatattgag gacggctccg tgcagctcgc ggatcattac
     1021 cagcagaaca cgccgatcgg ggacggccct gtcctcctgc cggataatca ctacctctcg
     1081 acgcagtctg cgctgtcaaa ggacccgaac gagaagaggg atcatatggt tctcctggag
     1141 ttcgtgacag ccgcggggat cactctcggc atggacgagc tgtacaaggg ggcgggcgac
     1201 tacaaggacg atgacgataa ggcttacccc tacgacgtgc cagattacgc tggggccggc
     1261 ggatccatgg acatgccgcc ggcggcgcag ggacggaggg cgatcgccgt ggtggaccgg
     1321 aagcttgacg agctctgcgc ctgcctcgac gacgcgatgt cgtccaggca gcggagcccg
     1381 tacgcggagg agcggctccg cgcggagatc aagtcgaggg ccgacttcct tcggtcgctc
     1441 atggccgccg aggtggactg ccacggcggg acgcgcccgg agcacctggc tgaggccgag
     1501 gcgcgcttcg ccgtcctaga ggccacgttc gatcggtggg cgcggcgcgc agtcgctgca
     1561 cctgaaccag tcgtcgagga gaaggaggag gaggccgact ccttggggtc ctcccggtcc
     1621 acgtgctcct gcaccgagtc gtgccaggag gcgacggagg gggacgccat gcgcgacgcc
     1681 gtggaaacac gcggcaagga ggggacgtcg gatggcatcg ccgaggacaa cgacgccgaa
     1741 tgcgagacgg tggtaatccg cagggaggag gcggcggcgg ttcctgttgc caagaagcgg
     1801 gatgaggcta tgggaacacg cgaggaggtg gcgctggacg ccattgccaa gaagaagggc
     1861 gataccgatc gcgatgccgc ggcggagacg gggaggagga ccgtagacca gcggcgctgg
     1921 tggaggcgga gtgctgcctg gtgcggtgca gccggcgtcg tcgcgttggt cgcggtcggg
     1981 ttggcggtgg agttggtcgc ggtgtagcct gcaggcagac ccagctttct tgtacaaagt
     2041 ggtgatatcc cgcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct
     2101 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     2161 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     2221 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     2281 aggcatgcga cgtcgggccc tctagaggat ccccgggtac cgcgaattat cgatcatgag
     2341 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac
     2401 gtttggaact gacagaaccg caacgttgaa ggagccactg agccgcgggt ttctggagtt
     2461 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     2521 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
     2581 tcggtatcca attagagtct catattcact ctcaactcga atccccccta tgaaaaagcc
     2641 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     2701 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     2761 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     2821 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggagttcag
     2881 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     2941 tgaaaccgaa ctgcccgctg ttcttcagcc ggtcgcggag gctatggatg ctatcgctgc
     3001 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     3061 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     3121 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     3181 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     3241 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     3301 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     3361 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccaa ggctccgggc
     3421 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     3481 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     3541 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     3601 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag
     3661 atgccgaccg aacaagagct gatttcgaga acgcctcagc cagcaactcg cgcgagccta
     3721 gcaaggcaaa tgcgagagaa cggccttacg cttggtggca cagttctcgt ccacagttcg
     3781 ctaagctcgc tcggctgggt cgcgggaggg ccggtcgcag tgattcagga attaattccc
     3841 tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     3901 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     3961 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     4021 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     4081 gcggtgtcat ctatgttact agatcgaccg gcatgcaagc tgataattca attcggcgtt
     4141 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     4201 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     4261 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     4321 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     4381 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     4441 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc
     4501 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     4561 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     4621 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     4681 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     4741 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     4801 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     4861 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     4921 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     4981 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     5041 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     5101 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     5161 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     5221 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     5281 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     5341 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     5401 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     5461 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     5521 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     5581 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     5641 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat
     5701 catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5761 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5821 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5881 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5941 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6001 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6061 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6121 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6181 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6241 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6301 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6361 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6421 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6481 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6541 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6601 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     6661 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     6721 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6781 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     6841 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     6901 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     6961 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     7021 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     7081 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     7141 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     7201 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
     7261 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     7321 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     7381 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     7441 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     7501 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     7561 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     7621 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     7681 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     7741 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     7801 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     7861 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     7921 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     7981 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     8041 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     8101 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
     8161 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
     8221 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
     8281 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
     8341 gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt
     8401 gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
     8461 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
     8521 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
     8581 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
     8641 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
     8701 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
     8761 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
     8821 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
     8881 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
     8941 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
     9001 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
     9061 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
     9121 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
     9181 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
     9241 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
     9301 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
     9361 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
     9421 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
     9481 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
     9541 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
     9601 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
     9661 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
     9721 tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct
     9781 cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct
     9841 cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct
     9901 ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg
     9961 tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc
    10021 gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca
    10081 ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg
    10141 cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc
    10201 ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca
    10261 gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg
    10321 ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat
    10381 agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc
    10441 aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg
    10501 cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca
    10561 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttaaa
    10621 gtactttaaa gtactttaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    10681 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
    10741 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
    10801 actgaaggcg ggaaacgaca atctgatcca agctcaagct aagcttgagc tctcccatat
    10861 ggtcgactag agccaagctg atctcctttg ccccggagat caccatggac gactttctct
    10921 atctctacga tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg
    10981 ataatgagaa gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag
    11041 aggcctacgc ggcaggtctc atcaagacga tctacccgag taataatctc caggagatca
    11101 aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga
    11161 acacagagaa agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag
    11221 gcttgcttca taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta
    11281 ctgaatcaaa ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga
    11341 agactggcga acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg
    11401 tcaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
    11461 aagaccaaag ggctat
//
