LOCUS       sequence-255923-        3883 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Synthetic source clone for maize Histon H2B.5 with C-terminal 
            mCherry fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Howe ES, Clemente TE, Bass HW
  TITLE     Maize histone H2B-mCherry: a new fluorescent chromatin marker for 
            somatic and meiotic chromosome research.
  JOURNAL   DNA Cell Biol. 2012 Jun;31(6):925-38. doi: 10.1089/dna.2011.1514. 
            Epub 2012 Jun 4.
  PUBMED    22662764
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/dna.2011.1514"; journalName: "DNA Cell Biol"; date: 
            "2012-06"; volume: "31"; issue: "6"; pages: "925-38"
COMMENT     Sequence Label: pHWBF01
FEATURES             Location/Qualifiers
     source          1..3883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..224
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     216..233
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     217..233
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1500..1516)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1500..1516)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1513..1535)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1524..1540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1548..1554,1555..1572,1573..1578))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1593..1614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1731..1748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1902..2490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1982..2001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2661..3452,3453..3521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3284..3303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3522..3626)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3694..3712
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3750..3772)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     join(3872..3883,1..8)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
       61 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
      121 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
      181 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
      241 ttgcatgcct gcaggtcgac tctagaggat ccatggcgcc caaggctgag aagaagccag
      301 ccgcgaagaa ggtggctgag gaggagccat ccgagaaggc tgcgccggcg gagaaggctc
      361 ctgctggcaa gaagcccaag gccgagaaga ggctcccagc ggggaagtca gcgggcaagg
      421 agggcgggga caagaagggc aggaagaagg ccaagaagtc cgtcgagacg tacaagatct
      481 acattttcaa ggtcctgaag caggttcacc cagacatcgg catttccagc aaggccatgt
      541 ctatcatgaa ctcattcatc aatgatatct tcgagaagct cgccgctgag gcggccaagc
      601 tggcccgcta caacaagaag cctacaatca cttcccggga gattcagacg agcgttcgcc
      661 tggtgctgcc cggggagctg gctaagcatg cggtctctga gggcacaaag gcggttacca
      721 agttcacgtc gtctgacccg cccgtggcta ctatggtctc caagggcgag gaggataaca
      781 tggcgatcat taaggagttc atgcggttca aggttcacat ggaggggtcc gtgaatggcc
      841 atgagttcga gatcgagggc gagggggagg gcaggccgta cgagggcacc cagaccgcta
      901 agctgaaggt gacgaagggc gggccactcc ctttcgcctg ggacatcctg tcgcctcagt
      961 tcatgtacgg gtctaaggcc tacgtgaagc acccggctga cattcccgat tacctcaagc
     1021 tgtccttccc ggagggcttc aagtgggaga gggttatgaa cttcgaggat ggcggggtgg
     1081 tcacagtgac tcaggactca tccctccagg atggcgagtt catctacaag gtcaagctgc
     1141 gggggaccaa tttcccaagc gacggccctg ttatgcagaa gaagacgatg ggctgggagg
     1201 ctagctcgga gaggatgtac ccggaggatg gggccctcaa gggcgagatc aagcagcggc
     1261 tcaagctgaa ggacggtggc cattacgatg ccgaggtgaa gaccacgtac aaggcgaaga
     1321 agcccgtgca gctcccgggc gcttacaacg tcaatatcaa gctggacatt acaagccaca
     1381 acgaggatta cactatcgtg gagcagtacg agagggccga gggcaggcat tccacgggcg
     1441 ggatggacga gctgtacaag tgaggatccc cgggtaccga gctcgaattc gtaatcatgg
     1501 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1561 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1621 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1681 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1741 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1801 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2341 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2461 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2701 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2761 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2821 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2881 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2941 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3001 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3061 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3121 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3181 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3241 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3301 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3361 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3421 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3481 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3541 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3601 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3661 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3721 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     3781 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     3841 gttggcgggt gtcggggctg gcttaactat gcggcatcag agc
//
