LOCUS       sequence-25599-e         775 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Dimitrova N, Chen YC, Spector DL, de Lange T
  TITLE     53BP1 promotes non-homologous end joining of telomeres by increasing
            chromatin mobility.
  JOURNAL   Nature. 2008 Oct 19. ():.
  PUBMED    18931659
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Oct 19. ():."
COMMENT     Sequence Label: N-Myc-53BP1 D1521A pLPC-Puro
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             83..112
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
       61 cgagctcgga tcagccacca tggaacaaaa gttgatttct gaagaagatt tgttaagaaa
      121 gagaggatcc gaccctactg gaagtcagtt ggattcagat ttctctcagc aagatactcc
      181 ttgcctgata attgaagatt ctcagcctga aagccaggtt ctagaggatg attctggttc
      241 tcacttcagt atgctatctc gacaccttcc taatctccag acgcacaaag aaaatcctgt
      301 gttggatgtt gtgtccaatc ctgaacaaac agctggagaa gaacgaggag acggtaatag
      361 tgggttcaat gaacatttga aagaaaacaa ggttgcagac cctgtggatt cttctaactt
      421 ggacacatgt ggttccatca gtcaggtcat tgagcagtta cctcagccaa acaggacaag
      481 cagtgttctg ggaatgtcag tggaatctgc tcctgctgtg gaggaagaga agggagaaga
      541 gttggaacag aaggagaaag agaaggaaga agatacttca ggcaatacta cacattccct
      601 tggtgctgaa gatactgcct catcacagtt gggttttggg gttctggaac tctcccagag
      661 ccaggatgtt gaggaaaata ctgtgccata tgaagtggnn aannnnnnnc tacaatcagt
      721 aaccaccaac tctggtnata ccnnntgtct gatgnnnngn nnatactgca attaa
//
