LOCUS       sequence-256121-        9618 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of BirA-GFP fusions  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Kulyyassov A, Ogryzko V
  TITLE     In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2
            and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation.
  JOURNAL   Biomolecules. 2020 Jan 16;10(1). pii: biom10010142. doi: 
            10.3390/biom10010142.
  PUBMED    31963153
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomolecules."; date: "2020-01-16"; volume: "10(1). pii: 
            biom10010142. doi"; pages: " 10.3390/biom10010142"
COMMENT     Sequence Label: pOz-humBirA-GFP
FEATURES             Location/Qualifiers
     source          1..9618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    70..619
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(214..231)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     441..460
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             1710..2179
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2875..2892)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2875..2891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2884..2906)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3100..3119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3219..3241
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3279..3297)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3365..3469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3470..3538,3539..4330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3688..3707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5362..5379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5496..5517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5532..5537,5538..5555,5556..5562)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5570..5586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5575..5597
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5594..5610
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5594..5610
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             6136..6605
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6670..7027
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7092..7508
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7438..7460
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7476..7492
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    7518..7892
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     7830..7846
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             7904..8866
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             8870..8893
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             join(8900..8902,8903..8905,8906..9616)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8900..8902,8903..8905,8906..9616)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8945..8966)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9206..9225)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9553..9574
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggccgcatcg agagatccgg gagacacaac ggtttccctc tagcgggatc aattccgccc
       61 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
      121 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
      181 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
      241 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
      301 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
      361 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
      421 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
      481 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
      541 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
      601 tttgaaaaac acgataatac catggattca tacctgctga tgtggggact gctcacgttc
      661 atcatggtgc ctggctgcca ggcagagctc tgtgacgatg acccgccaga gatcccacac
      721 gccacattca aagccatggc ctacaaggaa ggaaccatgt tgaactgtga atgcaagaga
      781 ggtttccgca gaataaaaag cgggtcactc tatatgctct gtacaggaaa ctctagccac
      841 tcgtcctggg acaaccaatg tcaatgcaca agctctgcca ctcggaacac aacgaaacaa
      901 gtgacacctc aacctgaaga acagaaagaa aggaaaacca cagaaatgca aagtccaatg
      961 cagccagtgg accaagcgag ccttccaggt cactgcaggg aacctccacc atgggaaaat
     1021 gaagccacag agagaattta tcatttcgtg gtggggcaga tggtttatta tcagtgcgtc
     1081 cagggataca gggctctaca cagaggtcct gctgagagcg tctgcaaaat gacccacggg
     1141 aagacaaggt ggacccagcc ccagctcata tgcacaggtg aaatggagac cagtcagttt
     1201 ccaggtgaag agaagcctca ggcaagcccc gaaggccgtc ctgagagtga gacttcctgc
     1261 ctcgtcacaa caacagattt tcaaatacag acagaaatgg ctgcaaccat ggagacgtcc
     1321 atatttacaa cagagtacca ggtagcagtg gccggctgtg ttttcctgct gatcagcgtc
     1381 ctcctcctga gtgggctcac ctggcagcgg agacagagga agagtagaag aacaatctag
     1441 aggatccgga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     1501 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     1561 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     1621 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     1681 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     1741 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     1801 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     1861 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     1921 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     1981 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
     2041 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     2101 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     2161 cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa
     2221 gtatttacat taaatggcca tagtacttaa agttacattg gcttccttga aataaacatg
     2281 gagtattcag aatgtgtcat aaatatttct aattttaaga tagtatctcc attggctttc
     2341 tactttttct tttatttttt tttgtcctct gtcttccatt tgttgttgtt gttgtttgtt
     2401 tgtttgtttg ttggttggtt ggttaatttt tttttaaaga tcctacacta tagttcaagc
     2461 tagactatta gctactctgt aacccagggt gaccttgaag tcatgggtag cctgctgttt
     2521 tagccttccc acatctaaga ttacaggtat gagctatcat ttttggtata ttgattgatt
     2581 gattgattga tgtgtgtgtg tgtgattgtg tttgtgtgtg tgattgtgta tatgtgtgta
     2641 tggttgtgtg tgattgtgtg tatgtatgtt tgtgtgtgat tgtgtgtgtg tgattgtgca
     2701 tgtgtgtgtg tgtgattgtg tttatgtgta taattgtgtg tgtgtgtgtg tgtgtgtgtg
     2761 tgtgtgtgtg tgtgtgtgtg ttgtgtatat atatttatgg tagtgagagg caacgctccg
     2821 gctcaggtgt caggttggtt tttgagacag agtctttcac ttagcttgga attcactggc
     2881 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2941 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     3001 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     3061 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     3121 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3181 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3241 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3301 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3541 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3601 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3661 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3721 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3781 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3841 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3901 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3961 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4021 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4081 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4141 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4201 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4261 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4321 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4621 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggccacgct tcccgaaggg
     5041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5401 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5461 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5521 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5581 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttg ctcttaggag
     5641 tttcctaata catcccaaac tcaaatatat aaagcatttg acttgttcta tgccctaggg
     5701 ggcgggggga agctaagcca gcttttttta acatttaaaa tgttaattcc attttaaatg
     5761 cacagatgtt tttatttcat aagggtttca atgtgcatga atgctgcaat attcctgtta
     5821 ccaaagctag tataaataaa aatagataaa cgtggaaatt acttagagtt tctgtcatta
     5881 acgtttcctt cctcagttga caacataaat gcgctgctga gaagccagtt tgcatctgtc
     5941 aggatcaatt tcccattatg ccagtcatat taattactag tcaattagtt gatttttatt
     6001 tttgacatat acatgtgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     6061 cattttgcaa ggcatggaaa aatacataac tgagaataga aaagttcaga tcaaggtcag
     6121 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     6181 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     6241 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     6301 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     6361 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     6421 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgattg
     6481 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     6541 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     6601 tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca
     6661 ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac
     6721 tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg
     6781 tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt
     6841 cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg
     6901 gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
     6961 tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg
     7021 tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa
     7081 atatgggccc gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     7141 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     7201 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     7261 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     7321 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     7381 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     7441 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     7501 taggcgcccc catatggcca tatgagatcc tatatggggc acccccgccc cttgtaaact
     7561 tccctgaccc tgacatgaca agagttacta acagcccctc tctccaagct cacttacagg
     7621 ctctctactt agtccagcac gaagtctgga gacctctggc ggcagcctac caagaacaac
     7681 tggaccgacc ggtggtacct cacccttacc gagtcggcga cacagtgtgg gtccgccgac
     7741 accagactaa gaacctagaa cctcgctgga aaggacctta cacagtcctg ctgaccaccc
     7801 ccaccgccct caaagtagac ggcatcgcag cttggataca cgccgcccac gtgaaggctg
     7861 ccgaccccgg gggtggacca tcctctagac tgccagatct accatgaagg acaacaccgt
     7921 gcccctgaag ctgatcgccc tgctggccaa cggcgagttc cactctggcg agcagctggg
     7981 agagaccctg ggaatgagca gagccgccat caacaagcac atccagacac tgagagactg
     8041 gggagtggac gtgttcaccg tgcctggcaa gggctacagc ctgcctgagc ctatccagct
     8101 gctgaacgcc aagcagatcc tgggacagct ggatggcgga agcgtggccg tgctgcctgt
     8161 gatcgactcc accaatcagt acctgctgga cagaatcgga gagctgaagt ccggcgacgc
     8221 ctgcatcgcc gagtaccagc aggctggcag aggacgcaga ggacggaagt ggttcagccc
     8281 attcggagcc aacctgtacc tgtccatgtt ctggagactg gagcagggac ctgctgctgc
     8341 catcggactg agtctggtga tcggaatcgt gatggccgag gtgctgagaa agctgggagc
     8401 cgacaaggtg agagtgaagt ggcctaatga cctgtacctc caggaccgca agctggctgg
     8461 catcctggtg gagctgacag gcaagacagg cgatgccgct cagatcgtga tcggagccgg
     8521 aatcaacatg gccatgagaa gagtggagga gagcgtggtg aaccagggct ggatcaccct
     8581 gcaggaggct ggcatcaacc tggaccggaa caccctggcc gccatgctga tcagagagct
     8641 gagagccgct ctggagctgt tcgagcagga gggactggct ccttacctga gcagatggga
     8701 gaagctggac aacttcatca acagacctgt gaagctgatc atcggcgaca aggaaatctt
     8761 cggcatctcc agaggaatcg acaagcaggg agctctgctg ctggagcagg acggaatcat
     8821 caagccctgg atgggcggag aaatctccct gagaagcgca gagaagtagc atcatcacca
     8881 tcaccaccat cacctcgaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     8941 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     9001 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     9061 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     9121 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     9181 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     9241 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     9301 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     9361 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     9421 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     9481 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     9541 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     9601 ggacgagctg tacaaggc
//
