LOCUS       sequence-256624-       10005 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Swap out effector and terminator to generate SPARC2-S CRISPR-donor 
            vector for CRISPR-HDR genomic insertion near the attP40 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE,
            Ishida IG, Maimon G, Wilson RI, Clandinin TR
  TITLE     SPARC enables genetic manipulation of precise proportions of cells.
  JOURNAL   Nat Neurosci. 2020 Sep;23(9):1168-1175. doi: 
            10.1038/s41593-020-0668-9. Epub 2020 Jul 20.
  PUBMED    32690967
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-020-0668-9"; journalName: "Nat Neurosci"; date: 
            "2020-09"; volume: "23"; issue: "9"; pages: "1168-1175"
COMMENT     Sequence Label: pHD-SPARC2-S-LexA::p65
FEATURES             Location/Qualifiers
     source          1..10005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2348..2442
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2476..2570
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2604..2698
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2732..2826
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2851..3089
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3036..3057
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          3298..3363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3493..3513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3785..3919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4322..4391
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             5448..5819
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     protein_bind    6123..6156
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6382..6391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6388..7065
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6518..6536)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6568..6588)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6786..6805
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6980..7003
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    7186..7307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7314..7347
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9006..9023)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9006..9022)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9121..9138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9292..9880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9372..9391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10004..10005,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttcg cacagaaaag catcttacgg atggtgtttg
     1261 ccaaaagtgc tggagatgtt ttagatatag atggtttatt ttaagaaatg gatgtatata
     1321 attgagctac tgcatcacgg gtttctgttt aacagtaaca gtgaaaagtg ctcaatgata
     1381 catatattac aaataataac ttttggtttt taaagtagat ttatataata taatcataat
     1441 tcttaatggg aatccatgtt acgaccaaat tagccgtggc ctttcaagtg gctgccactt
     1501 atattttaca actcgcacac agtggctcac accctcgtaa acaaaataat gctattaaat
     1561 caaattgaac atgtgaattc tctcaagaag acgccacttt tttttggggg gccagctgca
     1621 actgccacca atgtttactt ttatctttaa tgggcacatg ccttacatat acgaatttta
     1681 cacgattttt caagtggcgg tcgagagtgg ggtgataatt aattaaggtt gcctttagat
     1741 aaagtattat agagtttcaa aaaaatgaaa cttaagaatg gaatagctgc attccattct
     1801 atgtagattt cccttcaaaa ttattaaaat ccgttgacct ttgttcattt agcaattaga
     1861 attcgtctat gaataacaaa ttaaattgcg ttgcgggcaa attgctttag gccatttgaa
     1921 gtgcaacttt atatctcagg gccaacttct cggcgcgaca acaataaaaa catatacata
     1981 gatacacata tcagatgcaa tgcaatcacc acagcgtgac gtcattagtc taggcttgaa
     2041 aatacacggc aaacttgttt ataactctac gctgattaca taagccgcaa acacgacgaa
     2101 aaaacatttg ttttggtttc aaatccatga atgactttca ttaagtgcag ctgtaagctg
     2161 agctttgcca tgcgctgacc catattttca gcctctcgtt ctctctgttt cgcagatact
     2221 gtgaatatta gtaaaacgat ccataggggc tgtagtttat aatcataatc aaataggcat
     2281 aaacaaccac cagcgcggcc gcggacatat gggatccagt gcaccgtaag cttgcatgcc
     2341 tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     2401 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagccctag
     2461 ggcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     2521 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagactct
     2581 agcgctagcg catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     2641 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     2701 gagactctag cactagtgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
     2761 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     2821 tccgagcgga gactctagcg acgtcgagcg ccggagtata aatagaggcg cttcgtctac
     2881 ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa
     2941 ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat
     3001 caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag
     3061 taattattga atacaagaag agaactctga attaacgtaa gctagctaga ggatctttgt
     3121 gaaggaacct tacggtacca atcaaaagat ctactccagt ccaactgggg taacctttga
     3181 gttctctcag ttggtaatac gtaagctagc tagaggatct ttgtgaagga accttacttg
     3241 gtaccctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     3301 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     3361 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     3421 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     3481 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     3541 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     3601 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg
     3661 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3721 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3781 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3841 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3901 atcttatcat gtctggatcg atctggccgg cgggagtagt gccccaactg gggtaacctt
     3961 tgagttctct cagttggggg cgtagggtcg gggtcggcat ggcatctcca cctcctcgcg
     4021 gtccgacctg ggcatccgaa ggaggacgtc gtccactcgg atggctaagg gagagctcgg
     4081 atccgggcaa tacgaggtac tggcagacac gcagtgtcga acaacacctg acctaacggt
     4141 aagatgagta gataactcaa ctacttaaat cctggcctgc ttgatatctc gtagcgttct
     4201 tagagatcga cgaactgttt cacgaccggg tcggcatggc atctccacct cctcgcggtc
     4261 cgacctgggc atccgaagga ggacgtcgtc cactcggatg gctaagggag agctcggatc
     4321 cctcgaagcc gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca
     4381 cctcacccat cgtcgacatg aaggctctca cggcccgaca acaggaagtt tttgatttga
     4441 tacgggatca tatatcccaa acgggtatgc ctccgacccg cgcagagata gcacagcgac
     4501 tgggctttcg atcgcctaac gccgcggagg agcacttgaa ggcactggcc cgcaagggtg
     4561 tcattgaaat cgtgtccggt gcgagccgcg gaatccggct gttgcaggag gaggaagagg
     4621 gcctgccact ggtgggacgc gtggccgctg gcgagccgct gctggcccag caacacatag
     4681 agggacacta tcaggtggac ccctccttgt ttaagccaaa tgctgatttc ctgttgcggg
     4741 tgtcgggaat gtccatgaag gacatcggta ttatggatgg tgacctcctc gccgtccata
     4801 agacacagga tgtcaggaac ggccaggtag tcgttgccag gatagacgat gaggtcactg
     4861 tgaaacgtct caagaagcaa ggcaataagg tcgagctgct gccggagaat agcgagttca
     4921 agccgatcgt ggtggatctg cgacagcagt cctttactat cgagggcttg gccgtgggtg
     4981 tgatccgcaa cggagattgg ctgggatcca cgccgatgga gttccagtac ctgcccgata
     5041 cggatgaccg tcaccgtatc gaagaaaagc ggaagcgaac ctatgaaacc ttcaagtcca
     5101 tcatgaaaaa gtcccccttc tcgggcccca cggacccgcg ccccccgccc cgtcgtattg
     5161 cggttccttc gcgcagcagt gccagcgtcc ccaaacccgc accgcagccc tacccgttca
     5221 cttcctccct tagcacgatt aactatgatg agttccccac gatggtgttc cccagtggac
     5281 aaatttccca ggcatcggca ctggctccgg ccccaccgca agtcctcccc caggcgcccg
     5341 ctccggcacc ggctcccgca atggtgagtg ctctggccca ggcccccgct ccagtccccg
     5401 tgctggcgcc tggaccccca caggcagttg cccctcctgc tccgaaacca acgcaggcgg
     5461 gcgaaggaac cctgagcgag gccctcttgc agcttcagtt cgatgacgaa gacttgggag
     5521 ccctgctggg taacagcaca gaccctgccg tattcaccga tctcgcatcc gtggacaaca
     5581 gcgagtttca gcagctcttg aatcagggaa tcccggtcgc acctcatacc acagagccca
     5641 tgctgatgga atacccggag gctatcacgc gactggtgac cggcgcacag cgaccacccg
     5701 atccagcccc tgccccactg ggtgccccgg gtttgcccaa tggcctcctc agcggcgatg
     5761 aggatttctc cagcatcgct gatatggatt tctccgcttt gctgagccag ataagctcct
     5821 aagtttaaac aagcttatcg ataccgtcga ctaaagccaa atagaaaatt attcagttcc
     5881 tggcttaagt ttttaaaagt gatattattt atttggttgt aaccaaccaa aagaatgtaa
     5941 ataactaata cataattatg ttagttttaa gttagcaaca aattgatttt agctatatta
     6001 gctacttggt taataaatag aatatattta tttaaagata attgcgtttt tattgtcagg
     6061 gagtgagttt gcttaaaaac tcgtttagat ccactaggtc gactgaggca ctgcctcgag
     6121 agataacttc gtataatgta tgctatacga agttatcgta cgggatctaa ttcaattaga
     6181 gactaattca attagagcta attcaattag gatccaagct tatcgatttc gaaccctcga
     6241 ccgccggagt ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca
     6301 aagtgaacac gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa
     6361 acaatcggct cgaagccggt cgccaccatg gcctcctccg aggacgtcat caaggagttc
     6421 atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     6481 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     6541 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg
     6601 tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc
     6661 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     6721 tccctccagg acggctcctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc
     6781 gacggccccg taatgcagaa gaagactatg ggctgggagg cgtccaccga gcgcctgtac
     6841 ccccgcgacg gcgtgctgaa gggcgagatc cacaaggccc tgaagctgaa ggacggcggc
     6901 cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc
     6961 tactactacg tggactccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     7021 gagcagtacg agcgcgccga gggccgccac cacctgttcc tgtaggggcc gcgactctag
     7081 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     7141 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     7201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacc ggtataactt
     7321 cgtataatgt atgctatacg aagttatcac attgggtagc acagactctg agctttacta
     7381 cgattactat acagctcttc tctcgcgact ttttggactg gacaaggcgt agcacattga
     7441 acggcagtgg gtttgggttt agtatcgaac cggctttcta cgacagcgga ttggaagcgc
     7501 ggagcgacaa agtcgccggg taataatgtg atatagccgg ctatgttcag caggcaaaac
     7561 tgaaataaaa gtaataaaca ccgaaagccc ccgaattctc gcatcttctt ttgtgggact
     7621 gcgagaagaa ttgcgagcga aatactaatt tccttaccta actgtgtgag ataatctccg
     7681 gttggcattg tgtgtcgcgc aataaaatcc ccaaaagccc aaagcttcaa gtgtttaata
     7741 taataaaata gccaagaagc gtgtgtattt gcatttggga aaaatttaag gttgaattac
     7801 gtgggccgaa aaattaaaga ctgaaggcca ttcaagggtt gttcaaagag aaaattcggt
     7861 gaaaagtcac cgtgtgtgtg tgtgtgagtg tgtgtgcgtt gcgtgagaaa aatcaattct
     7921 gcatttggtg gaaagccgaa aacggagaga aaattggtgg agcgaggaga aaaggacacg
     7981 aattaaaggc tggttttttg gggctgcgaa aaactcgtga ccgatgacaa tttggcttga
     8041 cgagggtcta ttagcgaaat caatgctatt atggctgata gtggaggacc aggatccaag
     8101 cacatggatc caacaaccat agatgccggc gggggcggag caggagcagc cggaggtgat
     8161 gacgtgcctc cagtcccagc tgttcgccgg cgtcgtgcgc atgagcagaa atcctcgaga
     8221 gagcaggtgc tggaggagga gaagagccaa cagctggaga catcgacgat gacgaggacc
     8281 tacatgaaga ccatcaccac ctccctcacc acctcgagta gctccaacgt ggaagagttt
     8341 atcctggggg agcacgcagc aggagcagca gctgctccat cgcccaacca gcagcgattg
     8401 cgtcaaaagg tggcccagta cgagaaggtc tggtcggatg gctccagtcc cgttaagcgt
     8461 ccagccgagc agagctctga tgagctgcgt ctgaccgacg accaggatga gtacgccatc
     8521 cgtaagatgc ttttctgtgt caatcgagtt caagggcgac acaaaattta ttctaaatgc
     8581 ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc gttgacatgt
     8641 ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta ttttcttaat
     8701 tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata gatgaatagt
     8761 ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt gcgttgcttt
     8821 tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag gcgcccttaa
     8881 atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc cgaagcgggt
     8941 ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg gggcccgagc
     9001 ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa aaaggccatc
     9061 cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc cttccgcttc
     9121 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9181 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg ctaactacgg
     9721 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9901 tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg gattttggtc
     9961 atgagcttgc gccgtcccgt caagtcagcg taatgctctg ctttt
//
