LOCUS       sequence-256654-       14465 bp    DNA     circular SYN 14-APR-2025
DEFINITION  ORFeus expression, mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 14465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA025
FEATURES             Location/Qualifiers
     source          1..14465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      344..353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5279..5302
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5303..5368
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    5578..5712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5855..5877)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        10323..10427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10428..10496,10497..11288)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10646..10665)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11459..12047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11948..11967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12201..12218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12299..12318)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12337..12357)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12342..12671
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12522..12657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12764..13363
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12764..12783)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13260..13280
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    13593..13727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13792..13811)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13836..13884
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag cggccgcgtt taaacttaat taagccacca tgggcaagaa
      361 gcagaaccgc aagaccggca acagcaagac ccagagcgcc agcccccccc ccaaggagcg
      421 cagcagcagc cccgccaccg agcagagctg gatggagaac gacttcgacg agctgcgcga
      481 ggagggcttc cgccgcagca actacagcga gctgcgcgag gacatccaga ccaagggcaa
      541 ggaggtggag aacttcgaga agaacctgga ggagtgcatc acccgcatca ccaacaccga
      601 gaagtgcctg aaggagctga tggagctgaa gaccaaggcc cgcgagctgc gcgaggagtg
      661 ccgcagcctg cgcagccgct gcgaccaatt ggaggagcgc gtgagcgcca tggaggacga
      721 gatgaacgag atgaagcgcg agggcaagtt ccgcgagaag cgcatcaagc gcaacgagca
      781 gagcctgcag gagatctggg actacgtgaa gcgccccaac ctgcgcctga tcggcgtgcc
      841 cgagagcgac gtggagaacg gcaccaagct ggagaacacc ctgcaggaca tcatccagga
      901 gaacttcccc aacctggccc gccaggccaa cgtgcagatc caggagatcc agcgcacccc
      961 ccagcgctac agcagccgcc gcgccacccc ccgccacatc atcgtgcgct tcaccaaggt
     1021 ggagatgaag gagaagatgc tgcgcgccgc ccgcgagaag ggccgcgtga ccctgaaggg
     1081 caagcccatc cgtctcaccg ccgacctgag cgccgagacc ctgcaggccc gccgcgagtg
     1141 gggccccatc ttcaacatcc tgaaggagaa gaacttccag ccccgcatca gctaccccgc
     1201 caagctgagc ttcatcagcg agggcgagat caagtacttc atcgacaagc agatgctgcg
     1261 cgacttcgtg accacccgcc ccgccctgaa ggagctgctg aaggaggccc tgaacatgga
     1321 gcgcaacaac cgctaccagc ccctgcagaa ccacgccaag atgtaaagac catcgagact
     1381 aggaagaaac tgcatcaact aatgagcaaa atcaggcgcg cccatcataa tgaccggcag
     1441 caacagccac atcaccatcc tgaccctgaa catcaacggc ctgaacagcg ccatcaagcg
     1501 ccaccgcctg gccagctgga tcaagagcca ggaccccagc gtgtgctgca tccaggagac
     1561 ccacctgacc tgccgcgaca cccaccgcct gaagatcaag ggctggcgca agatctacca
     1621 ggccaacggc aagcagaaga aggccggcgt ggccatcctg gtgagcgaca agaccgactt
     1681 caagcccacc aagatcaagc gcgacaagga gggccactac atcatggtga agggcagcat
     1741 ccagcaggag gagctgacca tcctgaacat ctacgccccc aacaccggtg ccccccgctt
     1801 catcaagcag gtgctgagcg acctgcagcg cgacctggac agccacaccc tgatcatggg
     1861 cgacttcaac acccccctga gcaccctgga ccgcagcacc cgccagaagg tgaacaagga
     1921 cacccaggag ctgaacagcg ccctgcacca ggccgacctg atcgacatct accgcaccct
     1981 gcaccccaag agcaccgagt acaccttctt cagcgccccc caccacacct acagcaagat
     2041 cgaccacatc gtgggcagca aggccctgct gagcaagtgc aagcgcaccg agatcatcac
     2101 caactacctg agcgacgcta gcgccatcaa gctggagctg cgcatcaaga acctgaccca
     2161 gagccgcagc accacctgga agctgaacaa cctgctgctg aacgactact gggtgcacaa
     2221 cgagatgaag gccgagatca agatgttctt cgaaaccaac gagaacaagg acaccaccta
     2281 ccagaacctg tgggacgcct tcaaggccgt gtgccgcggc aagttcatcg ccctgaacgc
     2341 ctacaagcgc aagcaggagc gcagcaagat cgacaccctg accagccagc tgaaggagct
     2401 ggagaagcag gagcagaccc acagcaaggc cagccgccgc caggagatca ccaagatccg
     2461 cgccgagctg aaggagatcg agacccagaa gaccctgcag aagatcaacg agtcgcgaag
     2521 ctggttcttc gagcgcatca acaagatcga ccgccccctg gcccgcctga tcaagaagaa
     2581 gcgcgagaag aaccagatcg acaccatcaa gaacgacaag ggcgacatca ccaccgaccc
     2641 caccgagatc cagaccacca tccgcgagta ctacaagcac ctgtacgcca acaagctgga
     2701 gaacctggag gagatggaca ccttcctgga cacctacacc ctgccccgcc tgaaccagga
     2761 ggaggtggag agcctgaacc gccccatcac cggcagcgag atcgtggcca tcatcaacag
     2821 cctgcccacc aagaagagcc ccggccccga cggcttcacc gccgagttct accagcgcta
     2881 caaggaggag ctggtgccct tcctgctgaa gctgttccaa agcatcgaga aggagggcat
     2941 cctgcccaac agcttctacg aggccagcat catcctgatc cccaagcccg gccgcgacac
     3001 caccaagaag gagaacttcc gccccatcag cctgatgaac atcgacgcca agatcctgaa
     3061 caagatcctg gccaaccgca tccagcagca catcaagaag ctgatccacc acgaccaggt
     3121 gggcttcatc cccgggatgc agggctggtt caacatccgc aagagcatca acgtgatcca
     3181 gcacatcaac cgcgccaagg acaagaacca catgatcatc agcatcgacg ccgagaaggc
     3241 cttcgacaag atccagcagc ccttcatgct gaagaccctg aacaagctgg gcatcgacgg
     3301 cacctacttc aagatcatcc gcgccatcta cgacaagccc accgccaaca tcatcctgaa
     3361 cggccagaag ctggaggcct tccccctgaa gaccggcacg cgtcagggct gccccctgag
     3421 ccccctgctg ttcaacatcg tgctggaggt gctggcccgc gccatccgcc aggagaagga
     3481 gatcaagggc atccagctgg gcaaggagga ggtgaagctg agcctgttcg ccgacgacat
     3541 gatcgtgtac ctggagaacc ccatcgtgag cgcccagaac ctgctgaagc tgatcagcaa
     3601 cttcagcaag gtgagcggct acaagatcaa cgtgcagaag agccaggcct tcctgtacac
     3661 caacaaccgc cagaccgaga gccagatcat gggcgagctg cccttcacca tcgctagcaa
     3721 gcgcatcaag tacctgggca tccagctgac ccgcgacgtg aaggacctgt tcaaggagaa
     3781 ctacaagccc ctgctgaagg agatcaagga ggagaccaac aagtggaaga acatcccctg
     3841 cagctgggtg ggccgcatca acatcgtgaa gatggccatc ctgcccaagg tgatctaccg
     3901 cttcaacgcc atccccatca agctgcccat gaccttcttc accgagctgg agaagaccac
     3961 cctgaagttc atctggaacc agaagcgcgc ccgcatcgcc aagagcatcc tgagccagaa
     4021 gaacaaggcc ggcggcatca ccctgcccga cttcaagctg tactacaagg ccaccgtgac
     4081 caagaccgcc tggtactggt accagaaccg cgatatcgac cagtggaacc gcaccgagcc
     4141 cagcgagatc atgccccaca tctacaacta cctgatcttc gacaagcccg agaagaacaa
     4201 gcagtggggc aaggacagcc tgttcaacaa gtggtgctgg gagaactggc tggccatctg
     4261 ccgcaagctg aagctggacc ccttcctgac cccctacacc aagatcaaca gccgctggat
     4321 caaggacctg aacgtgaagc ccaagaccat caagaccctg gaggagaacc tgggcatcac
     4381 catccaggac atcggcgtgg gcaaggactt catgagcaag acccccaagg ccatggccac
     4441 caaggacaag atcgacaagt gggacctgat caagctgaag agcttctgca ccgccaagga
     4501 gaccaccatc cgcgtgaacc gccagcccac cacctgggag aagatcttcg ccacctacag
     4561 cagcgacaag ggcctgatca gccgcatcta caacgagctg aagcagatct acaagaagaa
     4621 gaccaacaac cccatcaaga agtgggccaa ggacatgaac cgccacttca gcaaggagga
     4681 catctacgcc gccaagaagc atatgaagaa gtgcagcagc agcctggcca tccgcgagat
     4741 gcagatcaag accaccatgc gctaccacct gacccccgtg cgcatggcca tcatcaagaa
     4801 gagcggcaac aaccgctgct ggcgcggctg cggcgagatc ggcaccctgc tgcactgctg
     4861 gtgggactgc aagctggtgc agcccctgtg gaagagcgtg tggcgcttcc tgcgcgacct
     4921 ggagctggag atccccttcg accccgccat ccccctgctg ggcatctacc ccaacgagta
     4981 caagagctgc tgctacaagg acacctgcac ccgcatgttc atcgccgccc tgttcaccat
     5041 cgccaagacc tggaaccagc ccaagtgccc caccatgatc gattggatca agaagatgtg
     5101 gcacatctac accatggagt actacgccgc catcaagaac gacgagttca tcagcttcgt
     5161 gggcacctgg atgaagctgg agaccatcat cctgagcaag ctgagccagg agcagaagac
     5221 caagcaccgc atcttcagcc tgatcggcgg caacggcggc ggcagcggcg gcggcagcct
     5281 ggaagttctg ttccaggggc ccgactacaa agaccatgac ggtgattata aagatcatga
     5341 catcgattac aaggatgacg atgacaagta gacaatgaga tcacatggac acaggaaggg
     5401 gaatatcaca ctctggggac tgtggtgggg tcgggggagg ggggagggat agcattggga
     5461 gatataccta atgctagatg acacattagt gggtgcagcg caccagcatg gcacatgtat
     5521 acatatgtaa ctaacctgca caatgtgcac atgtacccta aaacttagag tataatggat
     5581 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     5641 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     5701 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     5761 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     5821 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5881 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5941 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     6001 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     6061 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     6121 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     6181 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     6241 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     6301 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     6361 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     6421 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     6481 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     6541 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     6601 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     6661 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     6721 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     6781 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     6841 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     6901 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     6961 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     7021 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     7081 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     7141 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     7201 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     7261 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     7321 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     7381 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     7441 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     7501 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     7561 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     7621 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     7681 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctrtatcyg
     7741 ggtagyatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     7801 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     7861 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     7921 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     7981 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     8041 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
     8101 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
     8161 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
     8221 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
     8281 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
     8341 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
     8401 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
     8461 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
     8521 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
     8581 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
     8641 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
     8701 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
     8761 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
     8821 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
     8881 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
     8941 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
     9001 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
     9061 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
     9121 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
     9181 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
     9241 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
     9301 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
     9361 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
     9421 cactgcctcc tcgaccccgg cctccacctc ctgctcctgc cnnncctcct gctcctgccc
     9481 ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct gctcctgttc
     9541 caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc cggacaccat
     9601 ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg cctcctcgtc
     9661 ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga ggtccactgc
     9721 cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt ccaggtcctg
     9781 tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca tctttattag
     9841 acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc
     9901 tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc atcctccgaa
     9961 ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg aacatcctca
    10021 agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt tttgctggac
    10081 ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat
    10141 gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat
    10201 aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
    10261 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
    10321 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10381 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10441 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10501 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10561 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10621 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
    10681 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10741 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10801 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10861 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10921 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
    10981 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11041 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11101 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11161 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11221 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11281 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11341 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11401 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11461 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11521 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11581 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    11641 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11701 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11761 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11821 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11881 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11941 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12001 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12061 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    12181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
    12241 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    12301 caatctgctc tgatgccgca tagttaagcc agctgtggaa tgtgtgtcag ttagggtgtg
    12361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    12421 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    12481 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    12541 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    12601 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    12661 gcttttgcaa aaagcttgca tgcctgcagg tcggccgcca cgaccggtgc cgccaccatc
    12721 ccctgaccca cgcccctgac ccctcacaag gagacgacct tccatgaccg agtacaagcc
    12781 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
    12841 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
    12901 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    12961 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    13021 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    13081 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    13141 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    13201 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    13261 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    13321 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
    13381 gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc acccggggcg
    13441 gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagaggatc ataatcagcc
    13501 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
    13561 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
    13621 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    13681 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcact cgccgatagt
    13741 ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg
    13801 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
    13861 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
    13921 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
    13981 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
    14041 ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
    14101 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca
    14161 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa
    14221 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct
    14281 ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
    14341 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct ctagaagctg
    14401 ggtaccagct gctagcaagc ttgctagcgg ccgaggcgta tcacgaggcc ctttcgtctt
    14461 cactc
//
