LOCUS       sequence-256702-       11510 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor plasmid of targeted ASAP2f knockin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11510)
  TITLE     Lian Lab CRISPR plasmids
REFERENCE   2  (bases 1 to 11510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYJ22-AAVS1-Puro-CAG-ASAP2f
FEATURES             Location/Qualifiers
     source          1..11510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..840
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    847..872
                     /label=SA
                     /note="splice acceptor site"
     CDS             896..949
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             959..1558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(959..978)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1455..1475
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1590..1607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1596..1820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1826..2205
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2207..2484
                     /label=chicken beta-actin promoter
     intron          2485..3501
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3425..3447
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3509..3528
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    4871..5459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4924..4944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5342..5353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5491..5967
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5690..5709)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6077..6096)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6142..6197
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6196..6215)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6558..6574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6582..6598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6606..6612,6613..6630,6631..6636))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6651..6672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6746..7582
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7616..7635)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7617..7635)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7642..7659)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7642..7658)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7651..7673)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7796..8098
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7977..7996
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(8501..8520)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9115..9134
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9495..10286,10287..10355))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10118..10137
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10479..11067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10968..10987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11221..11238
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11355..11376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11391..11396,11397..11414,11415..11421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11429..11445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11453..11469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11490..11508
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagctagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc agcattctct
       61 cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct ctacggctgg
      121 cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc tcttcccctt
      181 gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt ctcggcgctg
      241 caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc gggcatctct
      301 cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc ttctccttct
      361 ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt ctcccttgcg
      421 tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa ccccaaagta
      481 ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc tggacacccc
      541 gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc ccctaccccc
      601 cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct tccaggggtc
      661 cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt caggacagca
      721 tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata ttcccagggc
      781 cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc acagtggggc
      841 aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg
      901 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggctcgagat
      961 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     1021 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     1081 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1141 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1201 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1261 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1321 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1381 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1441 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1501 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     1561 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     1621 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1681 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1741 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1801 ggatgcggtg ggctctatgg gtctcgacat tgattattga ctagttatta atagtaatca
     1861 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1921 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1981 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2041 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2101 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2161 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     2221 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     2281 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccrggs
     2341 ggggsggggg ggggsggggg gsggggsggg gsgaggcgga gaggtgcggc ggcagccaat
     2401 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     2461 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
     2521 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     2581 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
     2641 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     2701 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
     2761 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
     2821 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     2881 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     2941 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     3001 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     3061 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggrgggctc gggggagggg
     3121 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     3181 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     3241 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     3301 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     3361 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     3421 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     3481 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     3541 tttggcaaag aattcgtcga atggagacga ctgtgaggta tgaacagggg tcagagctca
     3601 ctaaaacttc gagctctcca acagcagatg agcccacgat aaagattgat gatggtcgtg
     3661 atgagggtaa tgaacaagac agctgttcca ataccattag gagaaaaatt tccccgtttg
     3721 tgatgtcatt tggattcaga gtatttggag ttgtgcttat cattgtagac atcatagtgg
     3781 tgattgtgga tctggccatc agtgagaaga aaagaggcat tagagagatt cttgaaggtg
     3841 tttccctggc tatagcactc ttcttccttg ttgatgttct catgagagtg tttgttgaag
     3901 gcttcaagaa ctatttccgg tccaaactga atactttgga tgcagtcata gtagtgggca
     3961 ctctgctaat taatatgacc tactccttct ctgaccttag ctttaacagc cataacgtgt
     4021 atattaccgc ggataaacag aaaaacggca ttaaagcgaa ctttaccgtg cgccataacg
     4081 tggaagatgg cagcgtgcag ctggcggatc attatcagca gaacaccccg attggcgatg
     4141 gcccggtgct gctgccggat aaccattatc tgagcaccca gaccgtgctg agcaaagatc
     4201 cgaacgaaaa acgcgatcac atggtgctgc tggaatttgt gaccgcagcg ggcattacac
     4261 acggcatgga tgaactgtat ggcggcaccg gcggcagcgc gagccagggc gaagaactgt
     4321 ttaccggcgt ggtgccgatt ctggtggaac tggatggcga tgtgaacggc cataaattta
     4381 gcgtgcgcgg cgaaggcgaa ggcgatgcga ccattggcaa actgaccctg aaatttattt
     4441 gcaccaccgg caaactaccg gtgccgtggc cgaccctggt gaccacctta acctatggcg
     4501 tgcagtgctt tagccgctat ccggatcata tgaaacgcca tgattttttt aaaagcgcga
     4561 tgccggaagg ctatgtgcag gaacgcacca ttagctttaa agatgatggc aaatataaaa
     4621 cccgcgcggt ggtgaaattt gaaggcgata ccctggtgaa ccgcattgaa ctgaaaggca
     4681 ccgattttaa agaagatggc aacattctgg ggcataaact ggaatataac acagatcaga
     4741 tgccgcagat ggttactctt cttcgagttc tgagaattgt tatcttaata agaatatttc
     4801 gcctggcttc acagaagaaa caacttgaag tggtaaccta acgcgtgaat tcgatatcaa
     4861 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4921 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4981 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5041 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5101 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5161 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5221 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5281 ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5341 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5401 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5461 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg
     5521 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     5581 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     5641 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     5701 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     5761 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     5821 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     5881 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     5941 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccaggcg cgtgaattca
     6001 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     6061 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     6121 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     6181 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     6241 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     6301 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     6361 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     6421 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     6481 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     6541 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6601 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6661 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6721 cagcggatcg acagtactaa gctttactag ggacaggatt ggtgacagaa aagccccatc
     6781 cttaggcctc ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc
     6841 agattcctta tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac
     6901 ctctaaggtt tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg
     6961 gtgggaggga agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc
     7021 ctctcccaga acctgagctg ctctgacgcg gctgtctggt gcgtttcact gatcctggtg
     7081 ctgcagcttc cttacacttc ccaagaggag aagcagtttg gaaaaacaaa atcagaataa
     7141 gttggtcctg agttctaact ttggctcttc acctttctag tccccaattt atattgttcc
     7201 tccgtgcgtc agttttacct gtgagataag gccagtagcc agccccgtcc tggcagggct
     7261 gtggtgagga ggggggtgtc cgtgtggaaa actccctttg tgagaatggt gcgtcctagg
     7321 tgttcaccag gtcgtggccg cctctactcc ctttctcttt ctccatcctt ctttccttaa
     7381 agagtcccca gtgctatctg ggacatattc ctccgcccag agcagggtcc cgcttcccta
     7441 aggccctgct ctgggcttct gggtttgagt ccttggcaag cccaggagag gcgctcaggc
     7501 ttccctgtcc cccttcctcg tccaccatct catgcccctg gctctcctgc cccttcccta
     7561 caggggttcc tggctctgct ctaagggcga attcgcggcc gctaaattca attcgcccta
     7621 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7681 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7741 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt
     7801 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     7861 tattattgac acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     7921 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     7981 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     8041 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     8101 gtcaggcatg agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag
     8161 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     8221 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     8281 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     8341 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     8401 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     8461 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     8521 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     8581 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     8641 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     8701 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     8761 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     8821 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     8881 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     8941 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     9001 cgtgacccat gcatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     9061 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     9121 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     9181 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     9241 tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     9301 gtatttcaca ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9361 ttatttttct aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc
     9421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10021 gtaagatgct tttctgtgac tggtgaatac tcaaccaagt cattctgaga atagtgtatg
    10081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10381 agcatttatc agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10441 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10501 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10561 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10621 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10681 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10741 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10801 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10861 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10921 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10981 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11041 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    11101 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    11161 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    11221 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    11281 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    11341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    11401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    11461 agctatgacc atgattacgc caagctcaga attaaccctc actaaaggga
//
