LOCUS       sequence-256745-       10801 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Activation of Mafa via SAM system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10801)
  TITLE     Lian Lab CRISPR plasmids
REFERENCE   2  (bases 1 to 10801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYJ33-lenti-U6-Oct4-PE-acti-gRNA-DsRed
FEATURES             Location/Qualifiers
     source          1..10801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..80
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        155..259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(260..328,329..1120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(478..497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1291..1879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1780..1799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2033..2050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2167..2188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2203..2208,2209..2226,2227..2233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2241..2257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2246..2268
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2265..2281
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2265..2281
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2330..2451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2341..2360)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2395..2414
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2581..2955)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2974..3021)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(3069..3398)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3083..3218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3137..3156)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3383..3403
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3412..3840)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3522..3543)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3734..3753
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3886..4110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4099..4116
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4142..4322
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4827..4843
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4828..4844
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4847..5435
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4953..4964
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5362..5382
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    5460..5493
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5926..5982)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5992..6666)
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(6051..6074)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6249..6268)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6466..6486
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(6695..7906)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(6839..6858)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        7939..8187
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7939..7959
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8110..8129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8208..8283
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8294..8327
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8371..8488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8786..8830)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9015..9248
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9741..9866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9913..10093
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10111..10309)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10136..10156)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10311..10690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc
      121 gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      181 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      241 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      301 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      361 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      421 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      481 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      541 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      601 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      661 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      721 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      781 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      841 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      901 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      961 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1021 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1081 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1141 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1201 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1261 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1321 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1381 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1441 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1501 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1561 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1621 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1681 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1741 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1801 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1861 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1921 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1981 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2041 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2101 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2161 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2221 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2281 ccatgattac gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt
     2341 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2401 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc
     2461 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     2521 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg
     2581 tcagtcctgc tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa
     2641 ctcccgcccc cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa
     2701 gttcgtggac acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac
     2761 ccaggccagg gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac
     2821 gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg
     2881 gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact
     2941 ggtcaacttg gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta
     3001 tgccgatgat taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc
     3061 gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     3121 tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     3181 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     3241 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     3301 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     3361 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt
     3421 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3481 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3541 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3601 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3661 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3721 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3781 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3841 ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg
     3901 catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
     3961 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
     4021 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
     4081 caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc
     4141 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     4201 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     4261 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     4321 ccagtacagt ccggatgcag ctctcgggcc atgtgatgaa atgctaggcg gctgtcaaac
     4381 ctccactcta atacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac
     4441 aagcgggtgt tctctccttc attggcttct tctaccttct cttgctcaac tggtactagc
     4501 ttgtagcacc atccaaaggt cagtggatat ctgatccctg gccctggtgt gtagttctgc
     4561 caatcaggga agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt
     4621 tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca
     4681 gctgccttgt aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc
     4741 tcctcctctt gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt
     4801 gtgattgctc catgtttttc taggtctcga ggtcgacggt atcgatgcgg ggaggcggcc
     4861 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
     4921 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
     4981 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
     5041 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
     5101 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
     5161 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
     5221 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     5281 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     5341 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     5401 aatctttcac aaattttgta atccagaggt tgattatcga taagcttgat atcgaattca
     5461 taacttcgta taatgtatgc tatacgaagt tatctattat caactttagt tcctggtgta
     5521 cttgaggggg atgagttcct caatggtggt tttgaccagc ttgccattca tctcaatgag
     5581 cacaaagcag tcaggagcat agtcagagat gagctctctg cacatgccac aggggctgac
     5641 caccctgatg gatctgtcca cctcatcaga gtaggggtgc ctgacagcca caatggtgtc
     5701 aaagtccttc tgcccgttgc tcacagcaga cccaatggca atggcttcag cacagacagt
     5761 gaccctgcca atgtaggcct caatgtggac agcagagatg atctccccag tcttggtcct
     5821 gatggccgcc ccgacatggt gcttgttgtc ctcatagagc atggtgatct tctcagtggc
     5881 gacctccacc agctccagat cctgctgaga gatgttgaag gtcttcggtc caggattctc
     5941 ttcgacatct ccggcttgtt tcagcagaga gaagtttgtt gcgccggatc cctggaacag
     6001 gtggtggcgg gcctcggcgc gctcgtactg ctccaccacg gtgtagtcct cgttgtggga
     6061 ggtgatgtcc agcttggagt ccacgtagta gtagccgggc agcttcacgg gcttcttggc
     6121 catgtagatt gacttgaact ccaccaggta gtggccgccg cccttcagct tcagcgcctt
     6181 gtggatctcg cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg agggctccca
     6241 gcccagagtc ttcttctgca ttacggggcc gtcggagggg aagttcacgc cgatgaactt
     6301 cacgtggtag atgaaggtgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
     6361 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttctt
     6421 gtagtcgggg atgtcggcgg ggtgcttcac gtacaccttg gagccgtact ggaactgggg
     6481 ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcacctgca gcttggcggt
     6541 ctgggtgccc tcgtagggct tgccctcgcc ctcgccctcg atctcgaact cgtggccgtt
     6601 cacggagccc tccatgtgca ccttgaagcg catgaagggc ttgatgacgt tctcagtgct
     6661 atccattcta gagtcgacct gcagcccaag cttcgtctaa caaaaaagcc aaaaacggcc
     6721 agaatttagc ggacaattta ctagtctaac actgaaaatt acatattgac ccaaatgatt
     6781 acatttcaaa aggtgcctaa aaaacttcac aaaacacact cgccaacccc gagcgcatag
     6841 ttcaaaaccg gagcttcagc tacttaagaa gataggtaca taaaaccgac caaagaaact
     6901 gacgcctcac ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc aggagagcct
     6961 accctaggcc cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca
     7021 ggtggcccca ccctgcatta taagccaaca gaacgggtga cgtcacgaca cgacgagggc
     7081 gcgcgctccc aaaggtacgg gtgcactgcc caacggcacc gccataactg ccgcccccgc
     7141 aacagacgac aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg tccccagatg
     7201 gtgccccagc ccatctcacc cgaataagag ctttcccgca ttagcgaagg cctcaagacc
     7261 ttgggttctt gccgcccacc atgcccccca ccttgtttca acgacctcac agcccgcctc
     7321 acaagcgtct tccattcaag actcgggaac agccgccatt ttgctgcgct ccccccaacc
     7381 cccagttcag ggcaaccttg ctcgcggacc cagactacag cccttggcgg tctctccaca
     7441 cgcttccgtc ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc cagcagcccg
     7501 ctactcacca agtgacgatc acagcgatcc acaaacaaga accgcgaccc aaatcccggc
     7561 tgcgacggaa ctagctgtgc cacacccggc gcgtccttat ataatcatcg gcgttcaccg
     7621 ccccacggag atccctccgc agaatcgccg agaagggact acttttcctc gcctgttccg
     7681 ctctctggaa agaaaaccag tgccctagag tcacccaagt cccgtcctaa aatgtccttc
     7741 tgctgatact ggggttctaa ggccgagtct tatgagcagc gggccgctgt cctgagcgtc
     7801 cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg
     7861 aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccagat ctggagccga
     7921 cacgggttaa ttaattaaga gggcctattt cccatgattc cttcatattt gcatatacga
     7981 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     8041 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     8101 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     8161 tatatcttgt ggaaaggacg aaacaccagg gagaacgggg cctaccggtt ttagagctag
     8221 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     8281 tgcttttttt ttaataactt cgtataatgt atgctatacg aagttattaa ttaaccaaac
     8341 tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     8401 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     8461 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtctgcagaa
     8521 ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     8581 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     8641 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     8701 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     8761 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     8821 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     8881 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     8941 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     9001 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     9061 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     9121 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     9181 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     9241 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     9301 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     9361 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc
     9421 ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct
     9481 atcctttgat gcacacaata gagggttgct actgtattat ataatgatct aagttcttct
     9541 gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt
     9601 tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt
     9661 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
     9721 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
     9781 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
     9841 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
     9901 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     9961 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
    10021 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
    10081 accagagaga cccagtacag gcaaaacgcg ctgcttatat agacctccca ccgtacacgc
    10141 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
    10201 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
    10261 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
    10321 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
    10381 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
    10441 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
    10501 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
    10561 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
    10621 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
    10681 aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc
    10741 ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg
    10801 c
//
