LOCUS       sequence-256771-        8818 bp    DNA     circular SYN 15-APR-2025
DEFINITION  inducible NKX3-1 knock down.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8818)
  TITLE     Lian Lab CRISPR plasmids
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYJ35-EZ-Tet-pLKO-shNKX3.1-Puro
FEATURES             Location/Qualifiers
     source          1..8818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..164
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        453..547
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     464..483
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    501..519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    529..547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    650..767
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        816..1326
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1245..1263
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          1335..1907
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1915..1934
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1962..1980
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1991..2611
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2064..2083)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    2646..3219
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2813..2830)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3040..3059
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3239..3838
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3239..3258)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3735..3755
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3966..4199
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4271..4392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4308..4327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4362..4381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4432..4567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4588..4606)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4616..4633)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4616..4632)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4625..4647)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4774..5229
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4861..4880)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5071..5092
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5255..5359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5360..5428,5429..6220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5578..5597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6391..6979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6880..6899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7133..7150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7267..7288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7303..7308,7309..7326,7327..7333)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7341..7357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7346..7368
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7365..7381
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7365..7381
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7400..7420
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7402..7420
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7448..7674
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7675..7855
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7902..8027
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8520..8753
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
       61 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
      121 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
      181 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
      241 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
      301 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
      361 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
      421 atcgatcacg agactagcct cgagcggccg caatatttgc atgtcgctat gtgttctggg
      481 aaatcaccat aaacgtgaaa tccctatcag tgatagagac ttataagttc cctatcagtg
      541 atagagacgc tagcgagacc caagtgaaga tatggtacta gtccatatct tcacttgggt
      601 ctctttttga attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
      661 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      721 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      781 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
      841 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
      901 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
      961 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     1021 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     1081 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     1141 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     1201 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     1261 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     1321 ccccaggggg atctgtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa
     1381 aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc
     1441 ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac
     1501 aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct
     1561 tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc
     1621 tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt
     1681 agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct
     1741 ggcgtggaaa tattcttatt ggtagaaaca actacatcct ggtcatcatc ctgcctttct
     1801 ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac
     1861 cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1921 tgctggttat tgtgctgtct catcattttg gcaaagaatt gtaatacgac tcactatagg
     1981 gcgaattgat atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct
     2041 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
     2101 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
     2161 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     2221 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     2281 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     2341 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     2401 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     2461 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     2521 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     2581 agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc ggctcccggg agttctaggg
     2641 atctgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     2701 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     2761 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     2821 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     2881 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     2941 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     3001 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     3061 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     3121 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     3181 cacggggacg tggttttcct ttgaaaaaca cgatgataag gatccaccgg agcttaccat
     3241 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3301 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3361 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3421 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     3481 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     3541 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     3601 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     3661 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     3721 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     3781 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     3841 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc
     3901 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3961 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4021 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4081 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4141 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4201 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     4261 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4321 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4381 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     4441 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4501 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4561 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     4621 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4681 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4741 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4801 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4861 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4921 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4981 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5041 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5101 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5161 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     5221 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5281 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5341 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5401 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5461 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5521 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5581 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5641 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5701 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5761 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5821 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5881 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5941 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6001 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6061 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6121 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6181 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6241 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6301 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6361 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6421 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6481 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6541 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6601 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6661 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6721 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6781 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6841 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6901 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6961 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7021 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7081 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7141 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7201 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7261 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7321 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7381 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     7441 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     7501 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     7561 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     7621 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     7681 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7741 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7801 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     7861 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     7921 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     7981 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     8041 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     8101 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     8161 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     8221 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     8281 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     8341 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     8401 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     8461 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     8521 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     8581 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     8641 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     8701 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8761 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttgga
//
