LOCUS       sequence-257096-        9720 bp    DNA     circular SYN 15-APR-2025
DEFINITION  PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into delta-sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Zhao EM, Suek N, Wilson MZ, Dine E, Pannucci NL, Gitai Z, Avalos JL,
            Toettcher JE
  TITLE     Light-based control of metabolic flux through assembly of synthetic 
            organelles.
  JOURNAL   Nat Chem Biol. 2019 Jun;15(6):589-597. doi: 
            10.1038/s41589-019-0284-8. Epub 2019 May 13.
  PUBMED    31086330
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0284-8"; journalName: "Nat Chem Biol"; date: 
            "2019-06"; volume: "15"; issue: "6"; pages: "589-597"
COMMENT     Sequence Label: pNS1
FEATURES             Location/Qualifiers
     source          1..9720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        145..849
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1256..1267
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1527..2222
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             2235..3728
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     complement(3782..3798)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3783..3799)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4342..4361)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4343..4361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4368..4385)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4368..4384)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4377..4399)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4525..4980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4612..4631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4822..4843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5336..5355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5455..5477
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5515..5533)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5617..5721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5722..5790,5791..6582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5940..5959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6753..7341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7242..7261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7495..7512
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7629..7650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7665..7670,7671..7688,7689..7695)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7703..7719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7708..7730
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7727..7743
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7727..7743
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7764..7782
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(7990..8023)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(8075..8995)
                     /label=bleMX6
                     /note="yeast selectable marker conferring phleomycin 
                     resistance"
     terminator      complement(8075..8271)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(8277..8651)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(8652..8995)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(9050..9083)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctttattatg ttacaatatg gaagggaact ttacacttct cctatgcaca tatattaatt
       61 aaagtccaat gctagtagag aaggggggta acacccctcc gcgctctttt ccgatttttt
      121 tctaaaccgt ggaatatttc ggatatcctt ttgttgtttc cgggtgtaca atatggactt
      181 cctcttttct ggcaaccaaa cccatacatc gggattccta taataccttc gttggtctcc
      241 ctaacatgta ggtggcggag gggagatata caatagaaca gataccagac aagacataat
      301 gggctaaaca agactacacc aattacactg cctcattgat ggtggtacat aacgaactaa
      361 tactgtagcc ctagacttga tagccatcat catatcgaag tttcactacc ctttttccat
      421 ttgccatcta ttgaagtaat aataggcgca tgcaacttct tttctttttt tttcttttct
      481 ctctcccccg ttgttgtctc accatatccg caatgacaaa aaaatgatgg aagacactaa
      541 aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga gctgatgagg
      601 ggtatctcga agcacacgaa actttttcct tccttcattc acgcacacta ctctctaatg
      661 agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa aagtttgctg
      721 tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct cgtcattgtt
      781 ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat accaagcata
      841 caatcaactc cattgcggcc gctaaaatca tggctagcgc ctcaaacgat tatacccaac
      901 aagcaaccca aagctatggg gcctacccca cccagcccgg gcagggctat tcccagcaga
      961 gcagtcagcc ctacggacag cagagttaca gtggttatag ccagtccacg gacacttcag
     1021 gctatggcca gagcagctat tcttcttatg gccagagcca gaacacaggc tatggaactc
     1081 agtcaactcc ccagggatat ggctcgactg gcggctatgg cagtagccag agctcccaat
     1141 cgtcttacgg gcagcagtcc tcctatcctg gctatggcca gcagccagct cccagcagca
     1201 cctcgggaag ttacggtagc agttctcaga gcagcagcta tgggcagccc cagagtggga
     1261 gctacagcca gcagcctagc tatggtggac agcagcaaag ctatggacag cagcaaagct
     1321 ataatccccc tcagggctat ggacagcaga accagtacaa cagcagcagt ggtggtggag
     1381 gtggaggtgg aggtggaggt aactatggcc aagatcaatc ctccatgagt agtggtggtg
     1441 gcagtggtgg cggttatggc aatcaagacc agagtggtgg aggtggcagc ggtggctatg
     1501 gacagcagga ccgtggaggt ggaggaatgg tgagcgagct gattaaggag aacatgccca
     1561 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
     1621 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtcgtcgag ggcggccctc
     1681 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga accttcatca
     1741 agcaccctcc gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg
     1801 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     1861 aggacggctg cctcatctac aacgtcaagg ttagaggggt gaacttccca gccaacggcc
     1921 ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacgatg taccccgctg
     1981 acggcggcct ggaaggcgca tgtgacatgg ccctgaagct cgtgggcggg ggccacctga
     2041 tctgcaacct tgagaccaca tacagatcca agaaacccgc tacgaacctc aagatgcccg
     2101 gcgtctacaa cgtggaccac agactggaaa gaatcaagga ggccgacgat gagacctacg
     2161 tcgagcagca cgaggtggct gtggccagat actctactgg tggcgctggt gatggaggta
     2221 aagcggccac gcgtatgaag atggacaaaa agactatagt ttggtttaga agagacctaa
     2281 ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt tttcctgtct
     2341 tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca agatggtgga
     2401 tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct gacctcactt
     2461 taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt accggtgcta
     2521 caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac cataccgtaa
     2581 aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat ctattgtatg
     2641 aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat tcttactgga
     2701 agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg cggttgatgc
     2761 caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta gggctggaga
     2821 atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca ggatggagca
     2881 atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat gcaaagaaca
     2941 gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat ttcggggaaa
     3001 taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg gcaagagata
     3061 agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt ttaagagagt
     3121 attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg ttgagtcatc
     3181 ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga caaggcagga
     3241 ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga tggatgcata
     3301 acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat
     3361 ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt gacatccttg
     3421 gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc ttggacaatc
     3481 ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa tggcttcccg
     3541 agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct ttaaccgtac
     3601 tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgtg gacatcgaca
     3661 cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag atcatgatcg
     3721 gagcagcagc ccgggatcca ccggtcgcca ccgggcccta agaattcgat atcaagctta
     3781 tcgataccgt cgacctcgag taatgatcat ctctgctttt gtgcgcgtat gtttatgtat
     3841 gtacctctct ctctatttct atttttaaac caccctctca ataaaataaa aataataaag
     3901 tatttttaag gaaaagacgt gtttaagcac tgactttatc tactttttgt acgttttcat
     3961 tgatataatg tgttttgtct ctcccttttc tacgaaaatt tcaaaaattg accaaaaaaa
     4021 ggaatatata tacgaaaaac tattatattt atatatcata gtgttgataa aaaatgttta
     4081 tccattggac cgtgtatcat atttatcgcc ggcgaataaa ttaaggcgcg ccaacatatc
     4141 cggaggtacc ataaaacgga atgaggaata atcgtaatat tagtatgtag aaatatagat
     4201 tccattttga ggattcctat atcctcgagg agaacttcta gtatattctg tatacctaat
     4261 attatagcct ttatcaacaa tggaatccca acaattatct caacattcac ccatttctca
     4321 gtttaaacgg ccacgctggc cccctatagt gagtcgtatt acaattcact ggccgtcgtt
     4381 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4441 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4501 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4561 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4741 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4801 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4861 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4921 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4981 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtaata
     5041 actgatataa ttaaattgaa gctctaattt gtgagtttag tattaggatc tctacccttg
     5101 gcgaaaagtc ctctgccaac aatgatgata tctgatccac cacttacaac ttcgtcgagg
     5161 gttctgtact gctgacccaa tatgcgtata tataccaatc taagtctgtg ctccttcctt
     5221 cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa
     5281 aaaaaagaat aaaaaaaaaa tgatgaattg aaaaggtggt atggtgcact ctcagtacaa
     5341 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5401 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5461 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5521 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcacgcg
     5581 ccgaggacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5641 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5701 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5761 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5821 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5881 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5941 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6001 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6061 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6121 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6181 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6241 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6301 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6361 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6421 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6481 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6541 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6601 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6661 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6721 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6781 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6841 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6901 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6961 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7021 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7081 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7141 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7201 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7261 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7321 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7381 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7441 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7501 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7561 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7621 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7681 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7741 gaccatgatt acgccaagct cggaattaac cctcactaaa gggaacaaaa gctgggccgg
     7801 ccatttaaat gtttaaacaa cctgttggaa tagaaatcaa ctatcatcta ctaactagta
     7861 tttacattac tagtatatta tcatatacgg tgttagaaga tgacgcaaat gatgagaaat
     7921 agtcatctaa attagtggaa gctgaaacgc aaggattgat aatgtaatag gatcaatgaa
     7981 tataaacatt accgttcgta tagcatacat tatacgaagt tatattaagg gttctcgaga
     8041 gctcgggatg gcggcgttag tatcgaatcg acagcagtat agcgaccagc attcacatac
     8101 gattgacgca tgatattact ttctgcgcac ttaacttcgc atctgggcag atgatgtcga
     8161 ggcgaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
     8221 aaactataca aatgtcaagt tcttgaaaac aagaatcttt ttattgtcag tactgatcag
     8281 tcctgctcct cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc
     8341 cgcccccacg gctgctcgcc gatctcggtc atggccggcc cggaggcgtc ccggaagttc
     8401 gtggacacga cctccgacca ctcggcgtac agctcgtcca ggccgcgcac ccacacccag
     8461 gccagggtgt tgtccggcac cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg
     8521 tcccggacca caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg
     8581 gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac ggcgctggtc
     8641 aacttggcca tggttgttta tgttcggatg tgatgtgaga actgtatcct agcaagattt
     8701 taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt atatttctct
     8761 acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt ccctgctcgc
     8821 aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca aaatgtatgg
     8881 atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag tcacatcatg
     8941 cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc atgtcgctgg
     9001 ccgggtgacc cggcggggac gaggcaagct aaacagatct ctagacctaa taacttcgta
     9061 tagcatacat tatacgaacg gtacccggga aacagctgcg ccgggaaaca gctggccggg
     9121 atcgaagaaa tgatggtaaa tgaaatagga aatcaaggag catgaaggca aaagacaaat
     9181 ataagggtcg aacgaaaaat aaagtgaaaa gtgttgatat gatgtatttg gctttgcggc
     9241 gccgaaaaaa cgagtttacg caattgcaca atcatgctga ctctgtggcg gacccgcgct
     9301 cttgccggcc cggcgataac gctgggcgtg aggctgtgcc cggcggagtt ttttgcgcct
     9361 gcattttcca aggtttaccc tgcgctaagg ggcgagattg gagaagcaat aagaatgccg
     9421 gttggggttg cgatgatgac gaccacgaca actggtgtca ttatttaagt tgccgaaaga
     9481 acctgagtgc atttgcaaca tgagtatact agaagaatga gccaagactt gcgagacgcg
     9541 agtttgccgg tggtgcgaac aatagagcga ccatgacctt gaaggtgaga cgcgcataac
     9601 cgctagagta ctttgaagag gaaacagcaa tagggttgct accagtataa atagacaggt
     9661 acatacaaca ctggaaatgg ttgtctgttt gagtacgctt tcaattcatt tgggtgtgca
//
