LOCUS       sequence-257122-       10791 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans 
            germ line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Macias-Leon J, Askjaer P
  TITLE     Efficient FLP-mediated germ-line recombination in C. elegans.
  JOURNAL   MicroPubl Biol. 2018 Mar 19;2018:10.17912/W2G66S. doi: 
            10.17912/W2G66S.
  PUBMED    32550382
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2018-03-19"; volume: "2018:10.17912/W2G66S. doi"; 
            pages: " 10.17912/W2G66S"
COMMENT     Sequence Label: pBN396
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2265..2285
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3332..3356
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8987..8998
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9147..9171)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10632..10652)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10672..10688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10672..10688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10685..10707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctgacc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg cactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     2281 agttgggccc attaggtcag acaaaaattt ctgatcttta aaccttattg attaaaacta
     2341 atttttgccc gacttttttg acacatgcac tagacgcttg tactcaaaat ttggaaacct
     2401 tgctcaaatc cactactcag atatgacagt agcatcgata attgaaccat tatcctgaaa
     2461 tcccctttcc tgtcccggtt cattttccaa ttttttcaac ggtggaagaa ggggcaaatt
     2521 ttataccaaa acgtctccgt ttatgaccca ccccctatgc aaaataaacg gctctgggag
     2581 aaatagtaca gaaaacgcgg agcctgcttg attccttctc cgaggcgaca aaaaacgcat
     2641 cattcgtatt gtaagatgca tgtgtggctg gcctcttttg aattttactg gttttttata
     2701 tttctactaa tatttcgaaa aattttgtaa gtaaatcatt gattacgttt acaaaaattt
     2761 agaaacattt caaaatgatt acttctaaat taattatatt tacaggttag ttcgagttac
     2821 ttatgacctt ccaatccgcc atatccgcca aggacgctcc aattaatttt ttagaattat
     2881 tttttaacac atgtattatt ttttaacaat aaattattca tgaaatcatc aaatgtatat
     2941 gttctactat aatatttagg tcaaactata gaaaagaaat ccttgttttt ggaacaaagg
     3001 agttcagatc ctgtgcattt gatcttggat ccacatcttt ggtccatggg aaagcttggc
     3061 actgatcaag aaggattctt caaatatcta aaattgccgg ctgcccgtag aattccatca
     3121 gttttccaga gttttagtga aaatttgcat cccgttagaa acaatctaca caacggtaaa
     3181 aaggacccat tatttttacc gtaccgtttt cctttatggc cctctatgca aaaaattata
     3241 gcgtggaggg gcagtacaga ataataaagt gtgagaaaaa aaactgctct gcgagaaata
     3301 gtacagcaaa cgcggagcat gcttgattct atccactttg tacaagaaag ctgggttaat
     3361 acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc tcgaggagcc
     3421 ggctgtagca tgcggccgca tatcagtttt taaaaaatta aaccataaaa caaataatat
     3481 aacccaattt ttacatcaaa ccacaagaaa aaaatacatt tgggcccacg gataaagaaa
     3541 ttaaaaaaat acatttttta aaggcgcacc gaattaaaat tcatttgggt cttaccgcgt
     3601 ataccgtact ccgtttgttt gatcattttt gtcagcgctg gcggttgttt tttcatttca
     3661 tttctgcttc aaagacgttt tctcgaataa tttttcgttt attctctttt ttaaaattaa
     3721 tttctagccg taaatgttat aaattcaccc atttaacgca aatttcatgg taatctcatg
     3781 gaaaaatgca gtttctttgt taaagaaagc ttaaatagca aaaattcccc gactttcccc
     3841 aaaatcctgc tcgattttcc gttttctcat tgtattctct cttaattaat tttatcgata
     3901 atcaattgaa tgtttcagac agagaggtac caaaaatgcc acaattcgac attctctgta
     3961 agacaccacc aaaagtcctc gtccgtcaat tcgtcgagcg tttcgagcgt ccatccggag
     4021 agaagatcgc cctctgcgcc gccgagctca cctacctctg ctggatgatc acccacaacg
     4081 gaaccgccat caagcgtgcc accttcatgt cctacaacac catcatctcc aactccctct
     4141 ccttcgacat cgtcaacaag tccctccaat tcaagtacaa gacccaaaag gccaccatcc
     4201 tcgaggcctc cctcaaggta agtttaaaca tatatatact aactaaccct gattatttaa
     4261 attttcagaa gctcatccca gcctgggagt tcaccatcat cccatactac ggacaaaagc
     4321 accaatccga catcaccgac atcgtctcct ccctccaact ccaattcgag tcctccgagg
     4381 aggccgacaa gggaaactcc cactccaaga agatgctcaa ggccctcctc tccgagggag
     4441 agtccatctg ggagatcacc gagaagatcc tcaactcctt cgagtacacc tcccgtttca
     4501 ccaagaccaa gaccctctac caattcctct tcctcgccac cttcatcaac tgcggacgtt
     4561 tctccgacat caagaacgtc gacccaaagt ccttcaagct cgtccaaaac aagtacctcg
     4621 gagtcatcat ccaatgcctc gtcaccgaga ccaagacctc cgtctcccgt cacatctact
     4681 tcttctccgc ccgtggacgt atcgacccac tcgtctacct cgacgagttc ctccgtaact
     4741 ccgagccagt cctcaagcgt gtcaaccgta ccggaaactc ctcctccaac aagcaagagt
     4801 accaactcct caaggacaac ctcgtccgtt cctacaacaa ggccctcaag aagaacgccc
     4861 catactccat cttcgccatc aagaacggac caaagtccca catcggacgt cacctcatga
     4921 cctccttcct ctccatgaag gtaagtttaa acatgatttt actaactaac taatctgatt
     4981 taaattttca gggactcacc gagctcacca acgtcgtcgg aaactggtcc gacaagcgtg
     5041 cctccgccgt cgcccgtacc acctacaccc accaaatcac cgccatccca gaccactact
     5101 tcgccctcgt ctcccgttac tacgcctacg acccaatctc caaggagatg atcgccctca
     5161 aggacgagac caacccaatc gaggagtggc aacacatcga gcaactcaag ggatccgccg
     5221 agggatccat ccgttaccca gcctggaacg gaatcatctc ccaagaggtc ctcgactacc
     5281 tctcctccta catcaaccgt cgtatctaac gcgtgctgtc tcatcctact ttcacctagt
     5341 taactgcttg tcttaaaatc tatgcttctc tttagtatct aaaattttcc tagaagctta
     5401 caagtatata aatggtctct tctcaataaa ggttgtatat ttattcatct tattgaatct
     5461 gccatttcct cgtttttgcg agtttatata ccttccaatt ttctttctat tgtattttca
     5521 acttctaatt ttaattcagg gaaactgctt caacgcatca tggtgtcgaa gggagaagag
     5581 gataacatgg cttcactccc agctacacac gaactccaca tcttcggatc gatcaacgga
     5641 gtggatttcg atatggtcgg acaaggtaag tttaaacata tatatactaa ctaaccctga
     5701 ttatttaaat tttcaggaac tggaaaccca aacgatggat acgaggaact caacctcaag
     5761 tcgacaaagg gagatctgca attctcgcca tggattctcg tgccacacat cggatacgga
     5821 ttccaccaat acctcccata cccaggtaag tttaaactga gttctactaa ctaacgagta
     5881 atatttaaat tttcagatgg aatgtcacca ttccaagctg ccatggtgga tggatcggga
     5941 taccaagttc accgaacaat gcaattcgag gatggagcct cgctcacagt gaactaccga
     6001 tacacatacg agggatcgca catcaaggta agtttaaaca gttcggtact aactaaccat
     6061 acatatttaa attttcaggg agaggctcaa gttaagggaa caggattccc agctgatgga
     6121 ccagtgatga caaactcact cacagctgct gattggtgcc gatcgaaaaa gacataccca
     6181 aatgataaga caatcatctc gacattcaag tggtcgtaca ctactggaaa cggaaagcga
     6241 taccgatcga cagcccgaac aacatacaca ttcgctaagc caatggccgc caactacctc
     6301 aaggtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaatcaa
     6361 ccaatgtacg tgttccgaaa gacagaactc aagcactcaa agacagagct gaacttcaaa
     6421 gagtggcaaa aggccttcac agatgtgatg ggaatggatg aactctacaa gtaacgcgta
     6481 tgtatctgca tatccttcaa atgttgtcca tattgtatca gtaaattata atgccccctt
     6541 tttatatatt ctccttatca tgtaaatgtt ctattgattt tttgctggtg taaaacgttt
     6601 ttatagtaaa tcctctccac agtgaatttg ttaaattgta tcaaaacaag acattgatta
     6661 aatttgttcg agacttgtgg attgtaaaat attttgcaga aaatggcaat attcatttag
     6721 aaacattact tccataatag gaaaaactat ttatgagttt tcatgtgcct atgcaagttc
     6781 ccactctgtg tgaatttcat catacaaatt gagcaaacat aaggtttatg tccagtatgg
     6841 gttctcagat gttttgtaag agttgagctg tcggtgaatg ccttctcaca ttgagcacat
     6901 ggatacggtc tctctccagt gtgtgttctg aaataatgaa aaatagtctt gcaatatgtt
     6961 tctactattc gagaaaaaat gtaatttttt ttcttatggt aacccggctt tccccgggcc
     7021 tagttctaga cattctctaa tgaaaaaatc tttcagttga aattgaaaat gagttaaagt
     7081 tggagttttt attgaaaaca gatttccgtg tgattagtgt ttttagcgag tgtgacagga
     7141 cagcgaaaaa atatagaaac aaggggggaa ctgaaaagct taggaatgca ttgaacatga
     7201 gaaggggaag gggaaggaac aaactagaca ggaattattg gaatttaatc acatttggag
     7261 ttttttttct attcgacaga ataattatcc agaacatttt tgtattaaat atttatgcat
     7321 catatgagta gtcggctttg ttgtgcatga cgagtttgtt atcgacgaaa tagaagctgt
     7381 cagaacgagt ctcgtttgga ttgttgatca tgtcgtccac tgaaaaagag attagtcttt
     7441 tgaattgtac tttttagaat aatgactcac tgagttgttg agagagttga gggaactcat
     7501 agatatgttc acagttgttt cgtgaattcg gaatacagaa tccgaattca aagtcaaaac
     7561 acttcagaag gcgatctttg aagaagtgac gttcgatcat tcggaaatga tggattggga
     7621 tgtctcctac tttgaattcc acagttgctc cgaccgtttt gagtttcaaa aagtttggag
     7681 caaatctata acgcacgtat cttgccgact cctgtggcga ctcatcattc tcctgatcgt
     7741 tctccggttt ggcgatctcg aaaagcactt gctcagtgtc cagatcacgg atttggaact
     7801 tggtgaactc gatgttatag atgttcgcag atggggagca taagaatcct aaatttatgt
     7861 tttaaactga aatccaaagg gagcaagata ccttgagtga ttcccggaag tgctaaaacg
     7921 tcgttcggag tgatttgagc tttcttcgca agctccgatt ccgttgtgat tccttgttcg
     7981 gtgcttggtg gtggccgtgg catctggaaa tatggaaaag ttcaacaaaa agaaaagaga
     8041 aaagaatgaa atcggatatc aagagttagt tgagcggttt ctctagtttt ctgagtctca
     8101 cctgcgacgg gaaggtcgcc gagccgggtg gaatcgatcg ttgttgctcg gctttcatat
     8161 cggtttggtt ggaagcggct gaaaacggaa agaagtggaa gaaggaaaag agtgtggtgt
     8221 gacaggaaaa tggtaattag agggtgccaa ataaccagct atattttgtt tttttttgaa
     8281 aacattttta aaaagaaaaa tacgataatg atatcagatg gatttccgga aaactggtat
     8341 gaaaaatttc aacctttttg agtacatgta atcaaaatac actttgtaaa ttatcatttt
     8401 tattgaaact ccaccatttt tctatttata acgctaataa tttgaaaaag aaacctattg
     8461 cgaaccgcgg ggtgaatccc aaaaacgaat gcgttttggt ggagtgattg attcgaatcg
     8521 aagaagaaaa agaagaagac gtggaataga gagctcactc ttaaccgagc agcacacacc
     8581 gacagaaaaa aaaatgaaat gaatgagggt cttcttcttc ttcttcttcg aatgattgac
     8641 agaaatggga aaaagaggaa gattgagaag ggaaaaagga aggagaaaag aagcagaaga
     8701 agacgtcaga gaggagagga acgagcggaa aagcagcggg cgcaagtcat agaagtagca
     8761 gagctgggga gaagaagaca ctatccaaga aaggaatgac gagagagtat gcaaaggggt
     8821 atagggtgca gacagaatag gaacagaata acagatgatg agccaagaag agttgaaaag
     8881 ggcgatgaat ttgtcatgta acttaatttg ggtcaatttg agcatgatga attgaaatca
     8941 tcccttgttg ggagttaata accggtttgt tatcagaaac cctgtaatag aagggcgccc
     9001 taactttgag ccaattcatc ccggtttctg tcaaatatat caaaaagtgg tcaactgaca
     9061 aattgttttt gatattataa taaacatttt atccgttaac aattttcgaa tactttttac
     9121 aaggacttgg ataaattggc tcaaagagcc tgcttttttg tacaaacttg aattcaaaaa
     9181 cgcagaactc gtactgttgg atgcctgtgt agcggtcctc tattgatttt tgctattttt
     9241 tgataatttt actgtaaatt atgagttatt ccaattaaca ttcctagcta aatgtaagtt
     9301 agcgaccaat tttttagcaa ccccatttta tgacttttca gaatatcgcc tactgggtca
     9361 acgacacttg tttggaacta agggtcttcc caattttttt taaatcttca taggcttgac
     9421 agaatcaggg ttcttcaagc agctttgaga gggaatcaat tgcaacattt gatgttaaat
     9481 ttatattttt tatagttctc tggtgaaatg gggaccaccg caaagcccct tttatgtttt
     9541 taacttgaat aaaatcttgt attaaattgt gatttataga tctgtatggg taatttgaag
     9601 gaaaagaaag ggagctccca aaagtgcctt acagatccag gcccaataaa tgtaaactat
     9661 tgggcagtag tatggcaaaa atcgtgcgga atttgaaaaa cctagaatta aaataaatat
     9721 tcaacacaaa tgttcgagat attttaagtt cggagtgtat atctagaaca tattgtacaa
     9781 gtaggtctac gggatcgtct acgggaaaat ttgatttcct cgaagatgta tttaatgaat
     9841 tctctaatgt tgcaggtctt tgtatttgac agaatgttgg tttttgaaac ttattcgcca
     9901 taattgcttg gctactacat gtctcatgcc attgacacta tcctgtactc gaaatttgtt
     9961 aagtaactac ccaccgtcca cttatgacga gagggtcaat taaggtcact ccacaaaagt
    10021 aaatttgaaa acacatgaga aaaagtgatc tgaattccct tgaagtggct aaaacaaagc
    10081 agatgaggga tacgacgtat gcgactactg agagataagg tcgaaacttg ataaggatgc
    10141 tgcttacctg cttagcaagt ttcttcgcac aggagaaagt gacaagctaa actatcaaat
    10201 tcatctaaaa cctcaaaaaa ttattttgga tgaaataatg ttttattttg agcaacgcat
    10261 aaggcaaaga ataaattaaa ggcgatccgc ttagcggcga gttccgtcct cgaagaagat
    10321 tcctccatcg atggcgcagt actttggaca gattccacgc tctcctgggg ttcctggtgg
    10381 tccgactggt cctggctgtc ctgggtttcc gtcggctcct ggcttgccgt ctggtcctgg
    10441 tggtcccttt ggtccaggtt gtcctgaaag atgtggtgat tagatatgga ttgaaaaact
    10501 atgaaatgat tacctggggt tccgtcgttt cctggttgtc cggatggtcc tggtggtccg
    10561 actggtcctg gctctccggt ggatcctggc tctccctttt ggactggctc gcagacagct
    10621 ggaagtccct ccaacttttc tatacaaagt tgatagcttg gcgtaatcat ggtcatagct
    10681 gtttcctgtg tgaaattgtt atccgctggt atcagctcac tcaaaggcgg taatacggtt
    10741 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc a
//
