LOCUS       sequence-257325-        8910 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Myr mCherryDrTrkA expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pmCherry-Dr-TrkA
FEATURES             Location/Qualifiers
     source          1..8910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             934..1635
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(1073..1091)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1341..1360
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4342..4362
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     promoter        complement(4463..4481)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4464..4481)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4501..4518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4507..4731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4777..5205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4864..4883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5074..5095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5214..5234)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5219..5548
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5399..5534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5461..5480
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5615..6409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5669..5688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6279..6298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6583..6704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6620..6639)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6674..6693
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6753..6769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6753..6769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6766..6788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6777..6793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6801..6807,6808..6825,6826..6831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6846..6867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6984..7001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7155..7743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7235..7254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7914..8705,8706..8774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8537..8556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8775..8879)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 atgggatgta ttaaatcaaa aaggaaagac gctagcaagg gcgaggagga taacatggcc
      961 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     1021 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     1081 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     1141 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     1201 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     1261 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     1321 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     1381 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     1441 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     1501 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     1561 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     1621 gacgagctgt acaaggaatt cagtgctggt agtgctggta gtgctggcac cggtatgagc
     1681 cgggacccgt tgcccttttt tccaccgctt taccttggtg gcccggaaat taccaccgag
     1741 aactgcgagc gcgagccgat tcatattccc ggcagcatcc agccgcacgg cgccctgctc
     1801 actgccgacg ggcacagcgg cgaggtgctc cagatgagcc tcaacgcggc cacttttctg
     1861 ggacaggaac ccacagtgct gcgcggacag accctcgccg cactgctgcc cgagcagtgg
     1921 cccgcgctgc aagcggccct gccccccggc tgccccgacg ccctgcaata ccgcgcaacg
     1981 ctggactggc ctgccgccgg gcacctttcg ctgacggtgc accgggtcgg cgagttgctg
     2041 attctggagt tcgagccgac ggaggcctgg gacagcaccg ggccgcacgc gctgcgcaac
     2101 gcgatgttcg cgctcgaaag tgcccccaac ctgcgggcgc tggccgaggt ggcgacccag
     2161 acggtccgcg agctgacggg ctttgaccgg gtgatgctct acaaatttgc ccccgacgcc
     2221 accggcgaag tgattgccga ggcccgccgt gaggggctgc acgcctttct gggccaccgt
     2281 tttcccgcgt cggacattcc ggcgcaggcc cgcgcgctct acacccggca cctgctgcgc
     2341 ctgaccgccg acacccgcgc cgccgccgtg ccgctcgatc ccgtcctcaa cccgcagacg
     2401 aatgcgccca ccccgctggg cggcgccgtg ctgcgcgcca cctcgcccat gcacatgcag
     2461 tacctgcgga acatgggcgt cgggtcgagc ctgtcggtgt cggtggtggt cggcggccag
     2521 ctctggggcc tgatcgcctg ccaccaccag acgccctacg tgttgccgcc cgacctgcga
     2581 accacgctcg aatacctggg ccgcttgctg agcctgcaag ttcaggtcaa ggaagcggcg
     2641 gacgtggcgg cctttcgcca gagcctgcgg gagcaccacg cgcgggtggc cctcgcggcg
     2701 gcgcactcgc tctcgccgca cgacaccctc agtgacccgg cgcttgacct gctgggcctg
     2761 atgcgggccg ggggcctgat tctgcgtttc gagggccgct ggcagacgtt gggtgaagtg
     2821 ccgcctgccc cggcggtgga cgcgctgctg gcgtggctcg aaacccagcc gggcgccctg
     2881 gtccagaccg acgcgctagg ccaactgtgg cccgccggcg ccgatctcgc ccccagcgca
     2941 gcgggcctgc tcgccatcag cgtgggcgag ggctggtcgg agtgcctcgt ctggctgcgg
     3001 cccgaactgc ggctggaggt cgcctggggc ggggccactc ctgaccaggc gaaagacgac
     3061 ctcgggccgc gccactcatt cgacacctac ctcgaagaaa aacgcggcta cgccgagccc
     3121 tggcatcccg gcgaaatcga ggaggcgcag gatctacgtg acacattgac cggggcgctg
     3181 ggcgaggcag aggcagcagc aaaggaggca gcagcaaagg aagcggcggc gaaagaagcg
     3241 gcggcgaaag cgaacaaatg tggacagagg agcaaatttg ggatcaaccg ccctgctgtg
     3301 ctggcgccag aggatgggct ggccatgtcc ctacacttca tgacactggg tggcagttct
     3361 ctttccccta ctgagggcaa aggctccgga ctccagggcc acatcatgga gaacccacag
     3421 tacttcagtg atacctgtgt ccaccatatc aagcgccagg acatcattct caagtgggag
     3481 ctaggggagg gagcctttgg aaaggtcttt cttgctgagt gctacaacct tctgaatgat
     3541 caggacaaga tgctggtggc tgtcaaggca ctgaaggaga catctgagaa tgctcgtcag
     3601 gacttccatc gtgaggcaga gctgctcacc atgctacagc accaacacat cgtacgcttc
     3661 tttggagtct gcacggaggg tggcccattg ctcatggtct tcgagtacat gcgccatggg
     3721 gacctcaacc gtttcctccg gtcccacgga cctgatgcaa aactgctggc tggcggcgag
     3781 gatgtggctc ctggtccttt gggccttggg cagcttctgg ctgtggctag ccaggtggct
     3841 gctggtatgg tgtacctagc cagcctgcac tttgtgcacc gggacctggc cacacgcaac
     3901 tgtctggtgg gtcagggact agtggtcaag attggagact tcggcatgag cagggacatc
     3961 tacagcacag actactaccg tgtgggaggt cggaccatgc tgcccatccg ctggatgcct
     4021 ccagagagca tcctctaccg caagttcagc accgagagtg atgtgtggag cttcggggtg
     4081 gtgctctggg agatcttcac ctatggaaag caaccctggt accagctctc caacactgag
     4141 gcgatcgagt gcatcacgca gggccgggag ctggagcggc cgcgcgcctg ccctcctgat
     4201 gtctacgcca tcatgcgcgg ctgctggcag cgtgagccgc aacagcgcct cagcatgaag
     4261 gatgtgcacg cgcggctgca ggccttggca caggcgccac cgagttacct ggacgttctg
     4321 ggctagctcg agccaaccac tgaggatctg tactttcaga gcgataacga tggatccact
     4381 agtaacggcc gccagtgtgc tggaattctg cagatatcca tcacactggc ggccgctcga
     4441 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     4501 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4561 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4621 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4681 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4741 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4801 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4861 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4921 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4981 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5041 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5101 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5161 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     5221 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5281 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5341 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5401 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5461 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5521 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5581 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5641 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5701 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5761 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5821 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5881 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5941 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6001 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6061 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6121 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6181 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6241 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6301 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6361 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6421 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6481 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6541 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6721 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6841 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6901 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6961 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7021 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7081 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7141 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7201 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7261 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7321 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7381 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7441 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7501 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7561 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7621 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7681 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7741 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7801 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7861 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7921 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7981 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8041 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8101 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8161 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8221 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8281 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8341 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8401 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8461 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8521 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8581 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8641 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8701 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8761 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8821 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8881 acatttcccc gaaaagtgcc acctgacgtc
//
