LOCUS       sequence-257334-        3045 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  AtzC expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7036
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2046..2064)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2060..2107
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2125..2940)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2848..2867
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2932..2951
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgtgga gcccggacca tgtctaaaga cttcgattta
      721 attatccgca atgcatatct gtccgaaaaa gactccgttt acgatatcgg catcgtgggg
      781 gaccgtatca ttaaaattga ggcgaaaatc gaaggaaccg tgaaggacga gatcgatgcg
      841 aaaggtaacc ttgtttcgcc gggattcgta gatgcgcaca cgcacatgga caagagcttt
      901 acttctactg gggagcgcct tccgaaattt tggtcgcgcc cgtacacgcg tgacgctgcg
      961 attgaagatg gtttgaaata ttacaaaaat gctacccacg aagaaatcaa gcgccatgtt
     1021 attgaacacg cccatatgca ggtcttgcac ggcaccctgt acacccgtac gcatgttgac
     1081 gtagattctg ttgccaagac taaggctgta gaagccgtat tggaggcgaa ggaggaattg
     1141 aaagacctta tcgacatcca agtagtggca tttgcacaat caggtttctt tgtggatttg
     1201 gaatcggagt ctctgatccg caaaagcctg gatatgggct gtgacttagt tggcggggtt
     1261 gaccccgcta cccgtgagaa caatgttgag gggtcacttg acttatgttt caaattggca
     1321 aaggaatacg atgttgacat cgattaccac atccatgata tcggaactgt tggtgtatat
     1381 tcaattaacc gcttagctca gaagacgatc gagaacggat ataagggtcg cgtcacaaca
     1441 tcgcatgctt ggtgcttcgc ggatgctcca tcagaatggc tggatgaagc catcccattg
     1501 tataaggatt ccggtatgaa gtttgtaacg tgtttcagta gcacccctcc aacaatgcca
     1561 gtcattaagt tgttagaagc tggtatcaac ctgggttgcg ccagtgataa tatccgcgac
     1621 ttttgggttc cttttggaaa tggcgatatg gtccaagggg cgcttattga aacgcaacgc
     1681 ttggaactga agacgaaccg tgatttagga ttaatctgga aaatgatcac atcggagggg
     1741 gcccgtgtgc tgggaatcga gaagaactat ggcatcgaag tagggaagaa ggctgatctt
     1801 gtagttttga attcactgtc cccacagtgg gccattatcg accaagctaa acgcctttgt
     1861 gtcatcaaga acggacgtat tatcgtgaag gacgaggtga tcgtagctta acatggacga
     1921 gctgtacaag taactcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt
     1981 tttcgttttg gtccgtcgac cggctgctaa caaagcccga aaggaagctg agttggctgc
     2041 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     2101 ttttttgctg aaagccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa
     2161 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     2221 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     2281 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     2341 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     2401 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     2461 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     2521 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     2581 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     2641 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     2701 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     2761 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     2821 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     2881 atccatgttg gaatttaatc gcggcttcga gcaagacgtt tcccgttgaa tatggctcat
     2941 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     3001 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtg
//
