LOCUS       sequence-257435-        9796 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Bicistronic expression of Magneto2.0 and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 9796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pcDNA3.0-Magneto2.0-p2A-mCherry
FEATURES             Location/Qualifiers
     source          1..9796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      916..925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(922..3198,3199..3219,3220..3243,3244..3273,3274..3792,
                     3793..3813,3814..3837,3838..3861,3862..4410,4411..4431,
                     4432..4455,4456..4485,4486..4545)
                     /codon_start=1
                     /product="fusion between a C-terminally truncated rat TRPV4
                     cation channel, the human ferritin light chain, the human 
                     ferritin heavy chain, and a plasma membrane targeting 
                     signal from the Kir2.1 potassium channel (Wheeler et al., 
                     2016)"
                     /label=Magneto2.0
                     /note="can be used as a magnetically sensitive actuator of 
                     neuronal activity"
                     /translation="MADPGDGPRAAPGDVAEPPGDESGTSGGEAFPLSSLANLFEGEEG
                     SSSLSPVDASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPM
                     DSLFDYGTYRHHPSDNKRWRRKVVEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGST
                     ADLDGLLSYLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGN
                     MREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGY
                     FYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTK
                     FVTKMYDLLLLKCSRLFPDSNLETVLNNDGLSPLMMAAKTGKIGVFQHIIRREVTDEDT
                     RHLSRKFKDWAYGPVYSSLYDLSSLDTCGEEVSVLEILVYNSKIENRHEMLAVEPINEL
                     LRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVIT
                     LLTGVLFFFTSIKDLFMKKCPGVNSLFVDGSFQLLYFIYSVLVVVSAALYLAGIEAYLA
                     VMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVTL
                     LNPCTNMKVCNEDQSNCTVPSYPACRDSETFSAFLLDLFKLTIGMGDLEMLSSAKYPVV
                     FILLLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLR
                     KAFRSGARGGGGSDYKDDDDKGGGGSSRVMGSQIRQNYSTDVEAAVNSLVNLYLQASYT
                     YLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDE
                     WGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDH
                     LTNLHRLGGPEAGLGEYLFERLTLKHDARGGGGSDYKDDDDKGGGGSSRVMTTASTSQV
                     RQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAE
                     KLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKND
                     PHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNESARG
                     GGGSDYKDDDDKGGGGSSRVASKSRITSEGEYIPLDQIDINV"
     CDS             4561..4617
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4624..5331
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4766..4784)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5034..5053
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5349..5367)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5350..5367)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5387..5404)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5393..5617
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5663..6091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5750..5769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5960..5981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6100..6120)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6105..6434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6285..6420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6347..6366
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6501..7295
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6555..6574)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7165..7184
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7469..7590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7506..7525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7560..7579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7639..7655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7639..7655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7652..7674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7663..7679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7687..7693,7694..7711,7712..7717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7732..7753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7870..7887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8041..8629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8121..8140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8800..9591,9592..9660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9423..9442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9661..9765)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gggtctagag gtaccgccac catggcagat cctggtgatg gcccccgtgc agcgcctggg
      961 gatgtggctg agccccctgg agacgagagt ggcacttctg gtggggaggc cttccccctc
     1021 tcttccctgg ccaacctgtt tgagggagag gaaggctcct cttctctttc accagtggat
     1081 gctagccgcc ctgctggccc cggggatgga cgtccaaacc tgcgtatgaa gttccagggc
     1141 gctttccgca agggggttcc caaccccatt gacctgctgg agtccaccct gtatgagtcc
     1201 tcagtagtgc ctgggcccaa gaaagcgccc atggattcgt tgttcgacta tggcacttac
     1261 cggcaccacc ccagtgacaa caagagatgg aggaggaagg tcgtagagaa gcagccacag
     1321 agccccaaag ctcccgcccc ccagccaccc cccatcctca aagtcttcaa ccggcccatc
     1381 ctctttgaca tcgtgtcccg gggctccact gccgacctgg acggactgct ctcctacttg
     1441 ctgacccaca agaagcgcct gactgatgag gagttccggg aaccatccac agggaagacc
     1501 tgcctgccca aggcacttct gaacttaagc aatggccgaa acgacaccat cccagtgttg
     1561 ctggacattg cggaacgcac gggcaacatg cgggagttca tcaactcgcc cttcagagac
     1621 atctactacc gagggcagac ggcactgcac atcgccattg aacggcgctg caagcattac
     1681 gtggagctcc tggtggccca gggagccgat gtgcacgcgc aggcccgagg gcggttcttc
     1741 cagcccaagg atgagggtgg ctacttctac tttggggagc tgcccttgtc cttggcagcc
     1801 tgcaccaacc agccgcacat cgtcaactac ctgacagaga accctcacaa gaaagccgat
     1861 atgaggcgac aggactccag aggcaacacg gtgctccacg cgctggtggc catcgctgac
     1921 aacacccgag agaacaccaa gtttgtcacc aagatgtatg acctgttgct tctcaagtgc
     1981 tcccgcctct tcccagacag caacctggag actgtgctta acaatgacgg tctttcgccc
     2041 ctcatgatgg ctgccaagac tggcaagatc ggggtctttc agcacatcat ccgacgggag
     2101 gtgacagatg aggacacacg gcacctgtct cgcaagttca aggactgggc ctacgggcct
     2161 gtgtattctt ctctctacga cctctcctcc ctggatacgt gcggggagga agtgtccgtg
     2221 ctggagatcc tggtttacaa cagcaagatc gagaaccgcc atgagatgct ggctgtggag
     2281 cccattaacg aactgctgag ggacaagtgg cgtaagttcg gggccgtgtc cttctacatc
     2341 aacgttgtct cctatctgtg tgccatggtc atcttcaccc tcacagccta ctatcagcca
     2401 ctggagggca cgccacccta cccttaccgt accacggtgg actacctgag gctggctggt
     2461 gaggtcatca cgctcctcac aggagtcctg ttcttcttta ccagtatcaa agacttgttc
     2521 atgaagaaat gccctggagt gaattctctc ttcgtcgatg gctccttcca gttgctctac
     2581 ttcatctact cagtgctggt ggttgtgtct gcggcgctct acctggcagg gatcgaggcc
     2641 tatctggctg tgatggtctt tgccctggtc ctgggctgga tgaatgccct ttacttcacc
     2701 cgtgggctga agctgacagg gacctacagc atcatgattc agaagatcct cttcaaagat
     2761 ctcttccgct ttctgctggt ctacctgctt tttatgattg gctatgcctc agctctggtc
     2821 accctcctga atccgtgcac caacatgaag gtctgtaacg aggaccagag caactgcacg
     2881 gtgccctcat accccgcgtg ccgggacagc gagaccttca gcgccttcct actggacctc
     2941 ttcaagctca ccatcggcat gggcgacctg gagatgctga gcagcgctaa gtaccccgtg
     3001 gtcttcattc tcctgctggt tacctacatc atcctcacct tcgtgctcct gctgaacatg
     3061 ctcatcgccc tcatgggtga gaccgtgggc caggtgtcca aggagagcaa gcacatctgg
     3121 aagctgcagt gggccaccac catcctggac atcgagcgct ccttccctgt gttcctgagg
     3181 aaggccttcc gctccggagc acggggcggg ggcggttctg attataagga cgacgacgac
     3241 aaaggaggag gcggctcctc tcgggttatg ggctcccaga ttcgtcagaa ttattccacc
     3301 gacgtggagg cagccgtcaa cagcctggtc aatttgtacc tgcaggcctc ctacacctac
     3361 ctctctctgg gcttctattt cgaccgcgat gatgtggctc tggaaggcgt gagccacttc
     3421 ttccgcgaat tggccgagga gaagcgcgag ggctacgagc gtctcctgaa gatgcaaaac
     3481 cagcgtggcg gccgcgctct cttccaggac atcaagaagc cagctgaaga tgagtggggt
     3541 aaaaccccag acgccatgaa agctgccatg gccctggaga aaaagctgaa ccaggccctt
     3601 ttggatcttc atgccctggg ttctgcccgc acggaccccc atctctgtga cttcctggag
     3661 actcacttcc tagatgagga agtgaagctt atcaagaaga tgggtgacca cctgaccaac
     3721 ctccacaggc tgggtggccc ggaggctggg ctgggcgagt atctcttcga aaggctcact
     3781 ctcaagcacg acgcccgcgg cggcggcggc tccgattata aagatgatga tgataaaggc
     3841 ggcggcggct cctcccgcgt catgacgacc gcgtccacct cgcaggtgcg ccagaactac
     3901 caccaggact cagaggccgc catcaaccgc cagatcaacc tggagctcta cgcctcctac
     3961 gtttacctgt ccatgtctta ctactttgac cgcgatgatg tggctttgaa gaactttgcc
     4021 aaatactttc ttcaccaatc tcatgaggag agggaacatg ctgagaaact gatgaagctg
     4081 cagaaccaac gaggtggccg aatcttcctt caggatatca agaaaccaga ctgtgatgac
     4141 tgggagagcg ggctgaatgc aatggagtgt gcattacatt tggaaaaaaa tgtgaatcag
     4201 tcactactgg aactgcacaa actggccact gacaaaaatg acccccattt gtgtgacttc
     4261 attgagacac attacctgaa tgagcaggtg aaagccatca aagaattggg tgaccacgtg
     4321 accaacttgc gcaagatggg agcgcccgaa tctggcttgg cggaatatct ctttgacaag
     4381 cacaccctgg gagacagtga taatgaaagc gcacgcgggg gtggcgggag cgactataaa
     4441 gacgacgatg acaaaggggg tggaggctcc tcccgagtgg ctagcaagag cagaatcacc
     4501 agcgagggcg agtacatccc cctggaccag atcgacatca acgtgggatc aggcagcggc
     4561 gccacgaact tctctctgtt aaagcaagca ggagacgtgg aagaaaaccc cggtcccgga
     4621 tccgtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     4681 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     4741 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     4801 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     4861 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     4921 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     4981 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     5041 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     5101 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     5161 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     5221 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     5281 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta aggcgcgccg
     5341 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     5401 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5461 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5521 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5581 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5641 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6001 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6061 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     6121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6181 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6241 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6361 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6421 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     6481 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6541 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6601 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6661 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6721 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6781 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6841 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6901 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6961 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7021 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7081 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7141 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7201 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7261 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     7321 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     7381 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7441 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7501 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7561 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7621 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7681 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7741 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7801 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8461 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9781 agtgccacct gacgtc
//
