LOCUS       sequence-257541-        9844 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9844)
  TITLE     Leder Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV WS Full Myc (Wrn cDNA)
FEATURES             Location/Qualifiers
     source          1..9844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     934..950
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     934..950
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5329..5358
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5374..5391
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5435..5452)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5441..5665
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5711..6139
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5798..5817)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6008..6029
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6148..6168)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6153..6482
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6333..6468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6395..6414
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6549..7343
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6603..6622)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7213..7232
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7517..7638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7554..7573)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7608..7627
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7687..7703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7687..7703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7700..7722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7711..7727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7735..7741,7742..7759,7760..7765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7780..7801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7918..7935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8089..8677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8169..8188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8848..9639,9640..9708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9471..9490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9709..9813)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga cttgagcggc cgctctagaa ctagtggatc ccccgggctg
      961 caggaattcg gcacgagaaa gttagtaaat gtgaggcctg tctcgatgcc tgggtcctgg
     1021 gctttggttc tcagtcctcc ataaatcatc ctgctggagg agaagaccct tagatctggc
     1081 tcttctcagg ggcattttaa agacaaatga aaataaaatg gaaaccactt cactacagcg
     1141 gaaatttcca gaatggatgt ctatgcagag tcaaagatgt gctacagaag aaaaggcctg
     1201 cgttcagaag agtgttcttg aagataatct cccattctta gaattccctg gatccattgt
     1261 ttacagttat gaagctagtg attgctcctt cctgtctgaa gacattagca tgcgtctgtc
     1321 tgatggcgat gtggtgggat ttgacatgga atggccgccc atatacaagc cagggaaacg
     1381 aagcagagtc gcagtgatcc agttgtgtgt gtctgagaac aaatgttact tgtttcacat
     1441 ttcttccatg tcagttttcc cccagggatt aaaaatgtta ctagaaaaca aatcaattaa
     1501 gaaggcaggg gttgggattg aaggggacca gtggaaactt ctgcgtgatt ttgacgtcaa
     1561 gttggagagt tttgtggagc tgacggatgt tgccaatgaa aagttgaagt gcgcagagac
     1621 ctggagcctc aatggtctgg ttaaacacgt cttagggaaa caacttttga aagacaagtc
     1681 catccgctgc agcaattgga gtaatttccc cctcactgag gaccagaaac tgtatgcagc
     1741 cactgatgct tatgctggtc ttatcatcta tcaaaaatta ggaaatttgg gtgatactgt
     1801 gcaagtgttt gctctaaata aagcagagga aaacctacct ctggagatga agaaacagtt
     1861 gaatttaatc tccgaagaaa tgagggatct agccaatcgt tttcctgtca cttgcagaaa
     1921 tttggaaact ctccagaggg ttcctgtaat attgaagagt atttcagaaa atctctgttc
     1981 attgagaaaa gtgatctgtg gtcctacaaa cactgagact agactgaagc cgggcagtag
     2041 ttttaattta ctgtcatcag aggattcagc tgctgctgga gaaaaagaga aacagattgg
     2101 aaaacatagt acttttgcta aaattaaaga agaaccatgg gacccagaac ttgacagttt
     2161 agtgaagcaa gaggaggttg atgtatttag aaatcaagtg aagcaagaaa aaggtgaatc
     2221 tgaaaatgaa atagaagata atctgttgag agaagatatg gaaagaactt gtgtgattcc
     2281 tagtatttca gaaaatgaac tccaagattt ggaacagcaa gctaaagaag aaaaatataa
     2341 tgatgtttct caccaacttt ctgagtattt atctcccaat gatgatgaga atgactcctc
     2401 ctatataatt gaaagtgatg aagatttgga aatggagatg ctgaagtctt tagaaaacct
     2461 aaatagtgac atggtggaac ccactcactc taaatggttg gaaatgggaa ccaatgggtg
     2521 tcttcctcct gaggaggaag atggacacgg aaatgaagcc atcaaagagg agcaggaaga
     2581 agaggaccat ttattgccgg aacccaacgc aaagcaaatt aattgcctca agacctattt
     2641 cggacacagc agttttaaac cggttcagtg gaaagtcatc cattctgtat tagaagagag
     2701 aagagataat gttgttgtca tggcaactgg atatgggaag agtctgtgct tccagtatcc
     2761 gcctgtttat acaggcaaga ttggcattgt catttcacct ctcatttcct taatggaaga
     2821 ccaagtcctc cagcttgagc tatccaatgt tccagcctgt ttacttggat ctgcacaatc
     2881 aaaaaatatt ctaggagatg ttaaattagg caaatatagg gtcatctaca taactccaga
     2941 gttctgttct ggtaacttgg atctactcca gaaacttgac tctagtattg gcatcactct
     3001 cattgctgtg gatgaggctc actgcatttc agagtggggc catgatttca gaagttcatt
     3061 caggatgctg ggctctctta aaacagcgct cccattggtt ccagtcattg cactctccgc
     3121 tactgcaagc tcttccatcc gggaagacat tataagctgc ttaaacctga aagaccctca
     3181 gatcacctgc actggatttg atcggccaaa tctgtactta gaagttggac ggaaaacagg
     3241 gaacatcctt caggatctaa agccgtttct cgtccgaaag gcaagttctg cctgggaatt
     3301 tgaaggtcca accatcatct attgtccttc gagaaaaatg acagaacaag ttactgctga
     3361 acttgggaaa ctgaacttag cctgcagaac ataccacgct ggcatgaaaa ttagcgaaag
     3421 gaaggacgtt catcataggt tcctgagaga tgaaattcag tgtgttgtag ctactgtagc
     3481 ttttggaatg ggcattaata aagctgacat tcgcaaagtt attcattatg gtgcgcctaa
     3541 ggaaatggaa tcctattacc aggaaattgg tagagctggc cgggatggac ttcagagttc
     3601 ctgtcacttg ctctgggctc cagcagactt taacacatcc aggaatctcc ttattgagat
     3661 tcacgatgaa aagttccggt tatataaatt aaagatgatg gtaaagatgg aaaaatacct
     3721 tcactccagt cagtgtaggc gacgaatcat cttgtcccat tttgaggaca aatgtctgca
     3781 gaaggcctcc ttggacatta tgggaactga aaaatgctgt gataattgca ggcccaggct
     3841 gaatcattgc cttactgcta acaactcaga ggacgcatcc caagactttg ggccacaagc
     3901 attccagcta ctgtctgctg tggacatcct gcaggagaaa tttggaattg ggattccgat
     3961 cttatttctc cgaggatcta attctcagcg tcttcctgat aaatatcggg gtcacaggct
     4021 ctttggtgct ggaaaggagc aagcagaaag ttggtggaag actctttctc accatctcat
     4081 agctgaagga ttcttggtag aggttcccaa ggaaaacaaa tatataaaga catgttccct
     4141 cacaaaaaag ggtagaaagt ggcttggaga agccagtttg cagtctcctc cgagccttct
     4201 ccttcaagct aatgaagaga tgtttccaag gaaagttctg ctaccaagtt ctaatcctgt
     4261 atctccagaa acgacgcaac attcctctaa tcaaaaccca gctggattaa ctaccaagca
     4321 gtctaatttg gagagaacgc attcttacaa agtgcctgag aaagtttctt ctgggactaa
     4381 cattcctaaa aaaagtgccg tgatgccgtc accaggaaca tcttccagcc ccttagaacc
     4441 tgccatctca gcccaagagc tggacgctcg gactgggcta tatgccaggt tggtggaagc
     4501 aaggcagaaa cacgctaata agatggatgt acctccagct attttagcaa caaacaaggt
     4561 tctgctggac atggctaaaa tgagaccgac tactgttgaa aacatgaaac agatcgacgg
     4621 tgtctctgaa ggcaaagctg ctctgttggc ccctctgttg ggagtcatca aacatttctg
     4681 tcaagtaact agtgttcaga cagacctcct ttccagtgcc aaacctcaca aggaacagga
     4741 gaaaagtcag gagatggaaa agaaagactg ctcactcccc cagtctgtgg ccgtcacata
     4801 cactctattc caggaaaaga aaatgccctt acacagcata gctgagaaca ggctcctgcc
     4861 tctcacagca gtcggcatgc acttagccca ggcggtgaaa gccggctacc ccctggatat
     4921 ggagcgagct ggcctgaccc cagagacttg gaagattatt atggatgtca tccgaaaccc
     4981 tcccatcaac tcagatatgt ataaagttaa actcatcaga atgttagttc ctgaaaacat
     5041 cgacacgtac ctcatccaca tggcgattga gattcttcag agtggttccg acagcagaac
     5101 ccagcctcct tgtgattcca gcaggaagag gcgtttcccc agctctgcag agagttgtga
     5161 gagctgtaag gagagcaaag aggtggtcac cgagaccaag gcatcatctt cagagtcaaa
     5221 gagaaaatta cctgagtggt ttgccaaagg aaatgtgccc tcagctgata ccggcagctc
     5281 atcatcaatg gccaagacca aaaagaaagg tctcttaagc ttgggcccga acaaaaactc
     5341 atctcagaag aggatctgaa tagcgccgtc gaccatcatc atcatcatca ttgagtttaa
     5401 acggtctcca gcttaagttt aaaccgctga tcagcctcga ctgtgccttc tagttgccag
     5461 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5521 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5581 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5641 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5701 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5761 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5821 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5881 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5941 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6001 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6061 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6121 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6241 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6301 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6361 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6421 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6481 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     6541 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccagccgct tgggtggaga
     6601 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6661 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6721 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6781 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6841 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6901 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6961 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7021 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     7081 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7141 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7201 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7261 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7321 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     7381 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7441 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7501 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7561 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7621 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7681 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7741 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7801 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7861 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7921 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7981 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8821 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8881 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8941 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9001 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9061 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9121 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9181 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9241 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9301 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9361 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9421 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9481 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9541 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9601 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9661 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9721 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9781 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9841 cgtc
//
