LOCUS       sequence-257544-        9021 bp    DNA     circular SYN 14-APR-2025
DEFINITION  C-to-T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Huang TP, Zhao KT, Miller SM, Gaudelli NM, Oakes BL, Fellmann C, 
            Savage DF, Liu DR
  TITLE     Circularly permuted and PAM-modified Cas9 variants broaden the 
            targeting scope of base editors.
  JOURNAL   Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y.
  PUBMED    31110355
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y."
COMMENT     Sequence Label: CP1041-CBEmax
FEATURES             Location/Qualifiers
     source          1..9021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        142..521
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        522..725
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     676..696
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     767..786
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        767..785
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             835..855
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             856..1539
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2680..4395
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5827..6075
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6106..6354
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6397..6417
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6426..6443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6466..6483)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6472..6696
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6767..6783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6767..6783)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6780..6802)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6791..6807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6815..6821,6822..6839,6840..6845))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6860..6881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6998..7015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7169..7757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7249..7268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7928..8719,8720..8788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8551..8570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8789..8893)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8972..8991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
       61 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      121 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      181 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      241 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      301 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      361 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      421 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      481 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      541 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      601 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      661 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      721 gagctggttt agtgaaccgt cagatccgct agagatccgc ggccgctaat acgactcact
      781 atagggagag ccgccaccat gaaacggaca gccgacggaa gcgagttcga gtcaccaaag
      841 aagaagcgga aagtctcctc agagactggg cctgtcgccg tcgatccaac cctgcgccgc
      901 cggattgaac ctcacgagtt tgaagtgttc tttgaccccc gggagctgag aaaggagaca
      961 tgcctgctgt acgagatcaa ctggggaggc aggcactcca tctggaggca cacctctcag
     1021 aacacaaata agcacgtgga ggtgaacttc atcgagaagt ttaccacaga gcggtacttc
     1081 tgccccaata ccagatgtag catcacatgg tttctgagct ggtccccttg cggagagtgt
     1141 agcagggcca tcaccgagtt cctgtccaga tatccacacg tgacactgtt tatctacatc
     1201 gccaggctgt atcaccacgc agacccaagg aataggcagg gcctgcgcga tctgatcagc
     1261 tccggcgtga ccatccagat catgacagag caggagtccg gctactgctg gcggaacttc
     1321 gtgaattatt ctcctagcaa cgaggcccac tggcctaggt acccacacct gtgggtgcgc
     1381 ctgtacgtgc tggagctgta ttgcatcatc ctgggcctgc ccccttgtct gaatatcctg
     1441 cggagaaagc agccccagct gaccttcttt acaatcgccc tgcagtcttg tcactatcag
     1501 aggctgccac cccacatcct gtgggccaca ggcctgaagt ctggaggatc tagcggagga
     1561 tcctctggca gcgagacacc aggaacaagc gagtcagcaa caccagagag cagtggcggc
     1621 agcagcggcg gcagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     1681 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     1741 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     1801 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
     1861 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
     1921 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
     1981 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
     2041 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
     2101 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
     2161 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
     2221 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
     2281 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
     2341 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
     2401 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
     2461 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
     2521 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
     2581 tacgagacac ggatcgacct gtctcagctg ggaggcgacg gcggatcagg aggctctggc
     2641 ggttcaggtg gatcaggcgg tagcggaggt tcaggtggtg acaagaagta cagcatcggc
     2701 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     2761 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     2821 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     2881 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     2941 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     3001 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     3061 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     3121 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     3181 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     3241 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     3301 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     3361 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     3421 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     3481 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     3541 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     3601 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     3661 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     3721 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     3781 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     3841 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     3901 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     3961 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     4021 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     4081 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     4141 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     4201 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     4261 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     4321 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     4381 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     4441 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     4501 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     4561 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     4621 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     4681 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     4741 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     4801 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     4861 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     4921 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     4981 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     5041 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     5101 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     5161 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     5221 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     5281 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     5341 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     5401 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     5461 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     5521 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     5581 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     5641 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     5701 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     5761 atcggcaagg ctaccgccaa gtacttcttc tacagcagcg gcgggagcgg cgggagcggg
     5821 gggagcacta atctgagcga catcattgag aaggagactg ggaaacagct ggtcattcag
     5881 gagtccatcc tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa gccagagtct
     5941 gacatcctgg tgcacaccgc ctacgacgag tccacagatg agaatgtgat gctgctgacc
     6001 tctgacgccc ccgagtataa gccttgggcc ctggtcatcc aggattctaa cggcgagaat
     6061 aagatcaaga tgctgagcgg aggatccgga ggatctggag gcagcaccaa cctgtctgac
     6121 atcatcgaga aggagacagg caagcagctg gtcatccagg agagcatcct gatgctgccc
     6181 gaagaagtcg aagaagtgat cggaaacaag cctgagagcg atatcctggt ccataccgcc
     6241 tacgacgaga gtaccgacga aaatgtgatg ctgctgacat ccgacgcccc agagtataag
     6301 ccctgggctc tggtcatcca ggattccaac ggagagaaca aaatcaaaat gctgtctggc
     6361 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtctaa
     6421 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     6481 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6541 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6601 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6661 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6721 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6841 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6901 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6961 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7021 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7501 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7561 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7621 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7681 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7741 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac actcagtgga
     7801 acaaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7861 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7921 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7981 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8041 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8101 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8161 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8221 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8281 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8341 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8401 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8461 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8521 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8581 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8641 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8701 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8761 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8881 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8941 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     9001 gccagtatct gctccctgct t
//
