LOCUS       sequence-258662-        3344 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express constitutively an NAD-dependent formate dehydrogenase from 
            Pseudomonas sp. 101 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Gleizer S, Ben-Nissan R, Bar-On YM, Antonovsky N, Noor E, Zohar Y, 
            Jona G, Krieger E, Shamshoum M, Bar-Even A, Milo R
  TITLE     Conversion of Escherichia coli to Generate All Biomass Carbon from 
            CO2.
  JOURNAL   Cell. 2019 Nov 27;179(6):1255-1263.e12. doi: 
            10.1016/j.cell.2019.11.009.
  PUBMED    31778652
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.11"; journalName: "Cell"; date: "2019-11-27- 
            27"; volume: "179"; issue: "6"; pages: "1255-1263.e12"
COMMENT     Sequence Label: pFDH
FEATURES             Location/Qualifiers
     source          1..3344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..792)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(793..884)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1014..1031
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2304..2390
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2555..3143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2635..2654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3231..3325)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
       61 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
      121 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
      181 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
      241 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
      301 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
      361 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
      421 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
      481 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
      541 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
      601 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
      661 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
      721 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
      781 cgcttccctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatctcga gacctattga
      901 caattaaagg ctaaaatgct ataattccac tcagcaggac gcactgaccg aattcactag
      961 ttaatagaaa taattttgtt taactttaaa caaaatgagg aggtactgag atgcatcatc
     1021 accatcacca cgctaaagtt ctgtgcgttc tgtacgacga cccggttgac ggttacccga
     1081 aaacctacgc tcgtgacgac ctgccgaaaa tcgaccacta cccgggtggt cagaccctgc
     1141 cgaccccgaa agctatcgac ttcaccccgg gtcagctgct gggttctgtt tctggtgaac
     1201 tgggtctgcg taaatacctg gaatctaacg gtcacaccct ggttgttacc tctgacaaag
     1261 acggtccgga ctctgttttc gaacgtgaac tggttgacgc tgacgttgtt atctctcagc
     1321 cgttctggcc ggcttacctg accccggaac gtatcgctaa agctaaaaac ctgaaactgg
     1381 ctctgaccgc tggtatcggt tctgaccacg ttgacctgca atctgctatc gaccgtaacg
     1441 ttaccgttgc tgaagttacc tactgcaact ctatctctgt tgctgaacac gttgttatga
     1501 tgatcctgtc tctggttcgt aactacctgc cgtctcacga atgggctcgt aaaggtggtt
     1561 ggaacatagc tgactgcgta agccacgctt acgacctgga agctatgcac gttggtaccg
     1621 ttgctgctgg tcgtatcggt ctggctgttc tgcgtcgtct ggctccgttc gacgttcacc
     1681 tgcactacac cgaccgtcac cgtctgccgg aatctgttga aaaagaactg aacctgacct
     1741 ggcacgctac ccgtgaagac atgtacccgg tttgcgacgt tgttaccctg aactgcccgc
     1801 tgcacccgga aaccgaacac atgatcaacg acgaaaccct gaaactgttc aaacgtggtg
     1861 cttacatcgt taacaccgct cgtggtaaac tgtgcgaccg tgacgctgtt gctcgtgctc
     1921 tggaatctgg tcgtctggct ggttatgcgg gtgacgtgtg gttcccccag ccggctccga
     1981 aagaccaccc gtggcgtacc atgccgtaca acggtatgac cccgcacatc tctggtacca
     2041 ccctgaccgc tcaggctcgt tacgctgctg gtacccgtga aatcctggaa tgcttcttcg
     2101 aaggtcgtcc gatccgtgac gaatacctga tcgttcaggg tggtgctctg gctggtaccg
     2161 gtgctcactc ttactctaaa ggtaacgcta ccggtggttc tgaagaagct gctaaattca
     2221 aaaaagctgt ttaagctagc gcggccgctc gaggaggtcg acctgcagcc cgggggatcc
     2281 catggtacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2341 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
     2401 agacctaggg cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2461 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2521 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2581 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2641 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2701 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2761 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2821 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2881 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2941 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3001 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3061 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3181 agtggaacga aaactcacgt taagggattt tggtcatgac tagtgcttgg attctcacca
     3241 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     3301 attactggat ctatcaacag gagtccaagc gagctcgtta gaca
//
