LOCUS       sequence-258731-       12385 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 
            series deletion cassettes, including KanMX, hphMX and natMX. Used to
            perform CRISPR-Swap of alleles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12385)
  AUTHORS   Lutz S, Brion C, Kliebhan M, Albert FW
  TITLE     DNA variants affecting the expression of numerous genes in trans 
            have diverse mechanisms of action and evolutionary histories.
  JOURNAL   PLoS Genet. 2019 Nov 18;15(11):e1008375. doi: 
            10.1371/journal.pgen.1008375. eCollection 2019 Nov.
  PUBMED    31738765
REFERENCE   2  (bases 1 to 12385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2019-11-18"; volume: "15(11):e1008375. doi"; pages: 
            " 10.1371/journal.pgen.1008375. eCollection 2019 Nov"
COMMENT     Sequence Label: pFA0055
FEATURES             Location/Qualifiers
     source          1..12385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..667
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             702..2420
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             4815..4835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4863..5050
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4918..4942)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(5168..5187)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(5192..5266)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(5287..5555)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(5586..5606)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5586..5604)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5625..5641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5625..5641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5638..5660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5649..5665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5673..5679,5680..5697,5698..5703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5718..5739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5856..5873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6027..6615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6107..6126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6786..7577,7578..7646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7409..7428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7647..7751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7778..9124
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     9165..9183
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9221..9243)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9343..9362
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(9856..10950)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(10951..11358)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(11659..12114)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11796..11817)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12008..12027
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12240..12262
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12254..12271
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12255..12271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12281..12300
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12281..12299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     12324..12340
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     12325..12341
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
      661 aaacaaaaga tctgatatgg atcgaattag atctcgccac catggacaag aagtattcta
      721 tcggactgga catcgggact aatagcgtcg ggtgggccgt catcactgac gagtacaagg
      781 tgccctctaa gaagttcaag gtgctcggga acaccgaccg gcattccatc aagaaaaatc
      841 tgatcggagc tctcctcttt gattcagggg agaccgctga agcaacccgc ctcaagcgga
      901 ctgctagacg gcggtacacc aggaggaaga accggatttg ttaccttcaa gagatattct
      961 ccaacgaaat ggcaaaggtc gacgacagct tcttccatag gctggaagaa tcattcctcg
     1021 tggaagagga taagaagcat gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg
     1081 cctatcacga gaaataccca accatctacc atcttcgcaa aaagctggtg gactcaaccg
     1141 acaaggcaga cctccggctt atctacctgg ccctggccca catgattaag ttcagaggcc
     1201 acttcctgat cgagggcgac ctcaatcctg acaatagcga tgtggataaa ctgttcatcc
     1261 agctggtgca gacttacaac cagctctttg aagagaaccc catcaatgca agcggagtcg
     1321 atgccaaggc cattctgtca gcccggctgt caaagagccg cagacttgag aatcttatcg
     1381 ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa cctgattgct ctttcacttg
     1441 ggctgactcc caatttcaag tctaatttcg acctggcaga ggatgccaag ctgcaactgt
     1501 ccaaggacac ctatgatgac gatctcgaca acctcctggc ccagatcggt gaccaatacg
     1561 ccgacctttt ccttgctgct aagaatcttt ctgacgccat cctgctgtct gacattctcc
     1621 gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc aatgattaag cggtatgatg
     1681 agcaccacca ggacctgacc ctgcttaagg cactcgtccg gcagcagctt ccggagaagt
     1741 acaaggaaat cttctttgac cagtcaaaga atggatacgc cggctacatc gacggaggtg
     1801 cctcccaaga ggaattttat aagtttatca aacctatcct tgagaagatg gacggcaccg
     1861 aagagctcct cgtgaaactg aatcgggagg atctgctgcg gaagcagcgc actttcgaca
     1921 atgggagcat tccccaccag atccatcttg gggagcttca cgccatcctt cggcgccaag
     1981 aggacttcta cccctttctt aaggacaaca gggagaagat tgagaaaatt ctcactttcc
     2041 gcatccccta ctacgtggga cccctcgcca gaggaaatag ccggtttgct tggatgacca
     2101 gaaagtcaga agaaactatc actccctgga acttcgaaga ggtggtggac aagggagcca
     2161 gcgctcagtc attcatcgaa cggatgacta acttcgataa gaacctcccc aatgagaagg
     2221 tcctgccgaa acattccctg ctctacgagt actttaccgt gtacaacgag ctgaccaagg
     2281 tgaaatatgt caccgaaggg atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg
     2341 caattgtgga ccttctgttc aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg
     2401 actatttcaa gaaaattgaa tgcttcgact ctgtggagat tagcggggtc gaagatcggt
     2461 tcaacgcaag cctgggtacc taccatgatc tgcttaagat catcaaggac aaggattttc
     2521 tggacaatga ggagaaagag gacatccttg aggacattgt cctgactctc actctgttcg
     2581 aggaccggga aatgatcgag gagaggctta agacctacgc ccatctgttc gacgataaag
     2641 tgatgaagca acttaaacgg agaagatata ccggatgggg acgccttagc cgcaaactca
     2701 tcaacggaat ccgggacaaa cagagcggaa agaccattct tgatttcctt aagagcgacg
     2761 gattcgctaa tcgcaacttc atgcaactta tccatgatga ttccctgacc tttaaggagg
     2821 acatccagaa ggcccaagtg tctggacaag gtgactcact gcacgagcat atcgcaaatc
     2881 tggctggttc acccgctatt aagaagggta ttctccagac cgtgaaagtc gtggacgagc
     2941 tggtcaaggt gatgggtcgc cataaaccag agaacattgt catcgagatg gccagggaaa
     3001 accagactac ccagaaggga cagaagaaca gcagggagcg gatgaaaaga attgaggaag
     3061 ggattaagga gctcgggtca cagatcctta aagagcaccc ggtggaaaac acccagcttc
     3121 agaatgagaa gctctatctg tactaccttc aaaatggacg cgatatgtat gtggaccaag
     3181 agcttgatat caacaggctc tcagactacg acgtggacca catcgtccct cagagcttcc
     3241 tcaaagacga ctcaattgac aataaggtgc tgactcgctc agacaagaac cggggaaagt
     3301 cagataacgt gccctcagag gaagtcgtga aaaagatgaa gaactattgg cgccagcttc
     3361 tgaacgcaaa gctaatcact cagcggaagt tcgacaatct cactaaggct gagaggggcg
     3421 gactgagcga actggacaaa gcaggattca ttaaacggca acttgtggag actcggcaga
     3481 ttactaaaca tgtagcccaa atccttgact cacgcatgaa taccaagtac gacgaaaacg
     3541 acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc caagctggtc agcgatttca
     3601 gaaaggactt tcaattctac aaagtgcggg agatcaataa ctatcatcat gctcatgacg
     3661 catatctgaa tgccgtggtg ggaaccgccc taatcaagaa gtacccaaag ctggaaagcg
     3721 agttcgtgta cggagactac aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc
     3781 aggagatcgg aaaggccacc gcaaagtact tcttctacag caacatcatg aatttcttca
     3841 agaccgaaat cacccttgca aacggtgaga tccggaagag gccgctcatc gagactaatg
     3901 gggagactgg cgaaatcgtg tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc
     3961 tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta
     4021 aggaatcaat cctccccaag cgcaactccg acaagctcat tgcaaggaag aaggattggg
     4081 accctaagaa gtacggcgga ttcgattcac caactgtggc ttattctgtc ctggtcgtgg
     4141 ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt gaaggaactg ctgggtatca
     4201 ccattatgga gcgcagctcc ttcgagaaga acccaattga ctttctcgaa gccaaaggtt
     4261 acaaggaagt caagaaggac cttatcatca agctcccaaa gtatagcctg ttcgaactgg
     4321 agaatgggcg gaagcggatg ctcgcctccg ctggcgaact tcagaagggt aatgagctgg
     4381 ctctcccctc caagtacgtg aatttcctct accttgcaag ccattacgag aagctgaagg
     4441 ggagccccga ggacaacgag caaaagcaac tgtttgtgga gcagcataag cattatctgg
     4501 acgagatcat tgagcagatt tccgagtttt ctaaacgcgt cattctcgct gatgccaacc
     4561 tcgataaagt ccttagcgca tacaataagc acagagacaa accaattcgg gagcaggctg
     4621 agaatatcat ccacctgttc accctcacca atcttggtgc ccctgccgca ttcaagtact
     4681 tcgacaccac catcgaccgg aaacgctata cctccaccaa agaagtgctg gacgccaccc
     4741 tcatccacca gagcatcacc ggactttacg aaactcggat tgacctctca cagctcggag
     4801 gggatgaggg agctcccaag aaaaagcgca aggtaggtag ttccggatcc tagactctcg
     4861 aggcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     4921 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     4981 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca
     5041 cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttctct agagcggccg
     5101 tggtatcgtt tagattggca attacagtgt cttagctcac atgcttataa ctaattacat
     5161 gactcgaaga cataaaaaac aaaaaaagca ccaccgactc ggtgccactt tttcaagttg
     5221 ataacggact agccttattt taacttgcta tttctagctc taaaactaat taacccgggg
     5281 atccgtgatc atttatcttt cactgcggag aagtttcgaa cgccgaaaca tgcgcaccaa
     5341 ctttcacttc tacagcgttt gaccaaaatc ttttgaacag aacattgtag ggtgtgaaaa
     5401 aatgcgcacc tttaccgcta gcccaagagg gcactacaaa atctagagtt gtacttcaaa
     5461 cgtacatgta atcaccttgt atatactcga aagaaaacat caagtttctg tataaatatg
     5521 agtgaaagca taatcataca ttatcttttc aaagattgtc cgcggtggag ctccagcttt
     5581 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     5641 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5701 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5761 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5821 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5881 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5941 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6001 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6061 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6121 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6181 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6241 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6301 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6361 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6421 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6481 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6541 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6601 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6781 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6841 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6901 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6961 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7021 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7081 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7141 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7201 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7261 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7321 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7381 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7441 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7501 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7561 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7621 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7741 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt
     7801 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     7861 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
     7921 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     7981 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
     8041 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
     8101 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
     8161 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
     8221 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
     8281 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
     8341 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
     8401 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
     8461 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
     8521 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     8581 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     8641 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     8701 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     8761 ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg
     8821 aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt
     8881 gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc
     8941 gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta
     9001 tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat
     9061 gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg
     9121 gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9181 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9241 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     9301 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     9361 tactgagagt gcaccatatc gactacgtcg ttaaggccgt ttctgacaga gtaaaattct
     9421 tgagggaact ttcaccatta tgggaaatgg ttcaagaagg tattgactta aactccatca
     9481 aatggtcagg tcattgagtg ttttttattt gttgtatttt ttttttttta gagaaaatcc
     9541 tccaatatat aaattaggaa tcatagtttc atgattttct gttacaccta actttttgtg
     9601 tggtgccctc ctccttgtca atattaatgt taaagtgcaa ttctttttcc ttatcacgtt
     9661 gagccattag tatcaatttg cttacctgta ttcctttaca tcctcctttt tctccttctt
     9721 gataaatgta tgtagattgc gtatatagtt tcgtctaccc tatgaacata ttccattttg
     9781 taatttcgtg tcgtttctat tatgaatttc atttataaag tttatgtaca aatatcataa
     9841 aaaaagagaa tctttttaag caaggatttt cttaacttct tcggcgacag catcaccgac
     9901 ttcggtggta ctgttggaac cacctaaatc accagttctg atacctgcat ccaaaacctt
     9961 tttaactgca tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca atttcaacat
    10021 cattgcagca gacaagatag tggcgatagg gttgacctta ttctttggca aatctggagc
    10081 agaaccgtgg catggttcgt acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa
    10141 ggacgcagat ggcaacaaac ccaaggaacc tgggataacg gaggcttcat cggagatgat
    10201 atcaccaaac atgttgctgg tgattataat accatttagg tgggttgggt tcttaactag
    10261 gatcatggcg gcagaatcaa tcaattgatg ttgaaccttc aatgtaggaa attcgttctt
    10321 gatggtttcc tccacagttt ttctccataa tcttgaagag gccaaaacat tagctttatc
    10381 caaggaccaa ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt
    10441 gattctttgc acttctggaa cggtgtattg ttcactatcc caagcgacac catcaccatc
    10501 gtcttccttt ctcttaccaa agtaaatacc tcccactaat tctctgacaa caacgaagtc
    10561 agtaccttta gcaaattgtg gcttgattgg agataagtct aaaagagagt cggatgcaaa
    10621 gttacatggt cttaagttgg cgtacaattg aagttcttta cggattttta gtaaaccttg
    10681 ttcaggtcta acactacctg taccccattt aggaccaccc acagcaccta acaaaacggc
    10741 atcagccttc ttggaggctt ccagcgcctc atctggaagt ggaacacctg tagcatctat
    10801 agcagcacca ccaattaaat gattttcgaa atcgaacttg acattggaac gaacatcaga
    10861 aatagcttta agaaccttaa tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg
    10921 caaaacgacg atcttcttag gggcagacat tagaatggta tatccttgaa atatatatat
    10981 atatattgct gaaatgtaaa aggtaagaaa agttagaaag taagacgatt gctaaccacc
    11041 tattggaaaa aacaataggt ccttaaataa tattgtcaac ttcaagtatt gtgatgcaag
    11101 catttagtca tgaacgcttc tctattctat atgaaaagcc ggttccggcg ctctcacctt
    11161 tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac ctctgaaatt
    11221 aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg tgtgttctcg
    11281 ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat cttacgatac
    11341 ctgagtattc ccacagttaa ctgcggtcaa gatatttctt gaatcaggcg ccttagaccg
    11401 ctcggccaaa caaccaatta cttgttgaga aatagagtat aattatccta taaatataac
    11461 gtttttgaac acacatgaac aaggaagtac aggacaattg attttgaaga gaatgtggat
    11521 tttgatgtaa ttgttgggat tccattttta ataaggcaat aatattaggt atgtagatat
    11581 actagaagtt ctcctcgacc ggtcgatatg cggtgtgaaa taccgcacag atgcgtaagg
    11641 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
    11701 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
    11761 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
    11821 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
    11881 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
    11941 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
    12001 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
    12061 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
    12121 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    12181 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    12241 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
    12301 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
    12361 cagtctacta gtcagttcga gttta
//
