LOCUS       sequence-258778-        2278 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Spacer cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0619
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(300..1091,1092..1160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     923..942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1161..1265)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1368..1956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1857..1876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggatcctaac tcgagatcga gctgagcatg agacggaaat ctgctcgtca gtggtgctca
       61 cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca actaagcaag
      121 acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca acagtccaac
      181 tagtgcactg cagtacagcg gccgcgatta tcaaaaagga tcttcaccta gatcctttta
      241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      421 agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac
      481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1261 ccgcgcacat ttccccgaaa agtgccacct gtcatgacca aaatccctta acgtgagttt
     1321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1501 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1741 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1921 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1981 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2041 tctgtggata accgtgcggc cgcccctgaa ttcgcatcta gaataaatcg cagccaagtg
     2101 agtgaataga tgacgcacca cggtcagaca cggccacatc gtatctcaca ggagcaagcg
     2161 cgataggagc actcacacat agtacggtga tccgctgact cctttgccca aataagacgt
     2221 gagcccgtct cagatgcaca cactggctta agatgacaac gactagagat ctatgtga
//
