LOCUS       sequence-259067-       11622 bp    DNA     circular SYN 14-APR-2025
DEFINITION  A binary plasmid for plant transformation containing _-glucuronidase
            gene driven by CaMV 35S promoter and a bialaphos resistance gene 
            (bar) driven by CaMV 35S promoter and TEV enhancer..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Frame BR, Shou H, Chikwamba RK, Zhang Z, Xiang C, Fonger TM, Pegg 
            SE, Li B, Nettleton DS, Pei D, Wang K
  TITLE     Agrobacterium tumefaciens-mediated transformation of maize embryos 
            using a standard binary vector system.
  JOURNAL   Plant Physiol. 2002 May;129(1):13-22. doi: 10.1104/pp.000653.
  PUBMED    12011333
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1104/pp"; 
            journalName: "Plant Physiol"; date: "2002-05"; volume: "129"; issue:
            "1"; pages: "13-22"
COMMENT     Sequence Label: pTF102
FEATURES             Location/Qualifiers
     source          1..11622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1217..1846
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2275..3348
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3414..3608
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3844..3866)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3952..4092
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4007..4026
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4107..4124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4278..4866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4358..4377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5112..5903)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6426..6450
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6650..6672
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6664..6681
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6665..6681
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7251..7799)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7951..8295)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          10103..10291
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     promoter        complement(10714..11058)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    11148..11204
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11213..11229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11213..11229)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11226..11248)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11237..11253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11261..11267,11268..11285,11286..11291))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11306..11327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    11485..11509
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttaa agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat
       61 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
      121 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
      181 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
      241 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
      301 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
      361 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
      421 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
      481 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
      541 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
      601 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
      661 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
      721 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
      781 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
      841 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
      901 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
      961 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     1021 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     1081 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     1141 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     1201 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     1261 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     1321 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     1381 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     1441 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     1501 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     1561 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     1621 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     1681 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     1741 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     1801 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     1861 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     1921 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     1981 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     2041 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     2101 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     2161 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     2221 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     2281 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     2341 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     2401 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     2461 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     2521 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     2581 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     2641 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     2701 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     2761 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     2821 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     2881 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     2941 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     3001 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     3061 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     3121 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     3181 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     3241 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     3301 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     3361 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     3421 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     3481 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     3541 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     3601 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     3661 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     3721 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     3781 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     3841 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     3901 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     3961 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     4021 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4081 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4681 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     4741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4921 cgttaaggga ttttggtcat gcatgatata tctcccaatt tgtgtagggc ttattatgca
     4981 cgcttaaaaa taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct
     5041 tagtgcatct aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattt
     5101 ctagctagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat
     5161 tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt
     5221 ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca
     5281 gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta
     5341 agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt
     5401 tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct
     5461 ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg
     5521 tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat
     5581 tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg
     5641 acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg
     5701 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca
     5761 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc
     5821 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact
     5881 tcggcgatca ccgcttcccc catgatgttt aactttgttt tagggcgact gccctgctgc
     5941 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     6001 ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc
     6061 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgacggc
     6121 agttacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg
     6181 cccgaattga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
     6241 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
     6301 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
     6361 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
     6421 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
     6481 ttgcggacgt ttttaatgta ctgaattaac gccgaattgc tctagcattc gccattcagg
     6541 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     6601 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6661 cgttgtaaaa cgacggccag tgccaagcta attcgcttca agacgtgctc aaatcactat
     6721 ttccacaccc ctatatttct attgcactcc cttttaactg ttttttatta caaaaatgcc
     6781 ctggaaaatg cactcccttt ttgtgtttgt ttttttgtga aacgatgttg tcaggtaatt
     6841 tatttgtcag tctactatgg tggcccatta tattaatagc aactgtcggt ccaatagacg
     6901 acgtcgattt tctgcatttg tttaaccacg tggattttat gacattttat attagttaat
     6961 ttgtaaaacc tacccaatta aagacctcat atgttctaaa gactaatact taatgataac
     7021 aattttcttt tagtgaagaa agggataatt agtaaatatg gaacaagggc agaagattta
     7081 ttaaagccgc gtaagagaca acaagtaggt acgtggagtg tcttaggtga cttacccaca
     7141 taacataaag tgacattaac aaacatagct aatgctccta tttgaatagt gcatatcagc
     7201 ataccttatt acatatagat aggagcaaac tctagctaga ttgttgagca gatctcggtg
     7261 acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg
     7321 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg
     7381 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc
     7441 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc
     7501 gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg
     7561 cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg
     7621 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc
     7681 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc
     7741 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg
     7801 gtagatcccc cgttcgtaaa tggtgaaaat tttcagaaaa ttgcttttgc tttaaaagaa
     7861 atgatttaaa ttgctgcaat agaagtagaa tgcttgattg cttgagattc gtttgttttg
     7921 tatatgttgt gttgagaatt aattctcgag gtcctctcca aatgaaatga acttccttat
     7981 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     8041 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     8101 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg tagaggcatc ttcaacgatg
     8161 gcctttcctt tatcgcaatg atggcatttg tagaagccat cttccttttc cactgtcttc
     8221 tcaataaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     8281 tgttgaaaag tctcaattgc cctttggtct tctgagactg tatctttgat atttttggag
     8341 tagacaagtg tgtcgtgctc caccatgttg accggcatgc aagcttgcat gcctgcaggt
     8401 cactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa
     8461 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata agaaccctaa
     8521 ttcccttatc tgggaactac tcacacatta ttctggagaa aaatagagag agatagattt
     8581 gtagagagag actggtgatt tttgcgccgg gtaccgagct cggtagcaat tcccgaggct
     8641 gtagccgacg atggtgcgcc aggagagttg ttgattcatt gtttgcctcc ctgctgcggt
     8701 ttttcaccga agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt
     8761 ttgcggtcgc gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag
     8821 gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag
     8881 acgcggtgat acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac
     8941 attgagtgca gcccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc
     9001 agtttctcct gccaggccag aagttctttt tccagtacct tctctgccgt ttccaaatcg
     9061 ccgctttgga cataccatcc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg
     9121 atggtatcgg tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg
     9181 tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg
     9241 gtatccggtt cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct
     9301 atcagctctt taatcgcctg taagtgcgct tgctgagttt ccccgttgac tgcctcttcg
     9361 ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag
     9421 ccgacagcag cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc
     9481 tcttcagcgt aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat
     9541 gcgtggtcgt gcaccatcag cacgttatcg aatcctttgc cacgtaagtc cgcatcttca
     9601 tgacgaccaa agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc
     9661 actgccactg accggatgcc gacgcgaagc gggtagatat cacactctgt ctggcttttg
     9721 gctgtgacgc acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc
     9781 acttgcaaag tcccgctagt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc
     9841 agttcaacgc tgacatcacc attggccacc acctgccagt caacagacgc gtggttacag
     9901 tcttgcgcga catgcgtcac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag
     9961 agcattacgc tgcgatggat tccggcatag ttaaagaaat catggaagta agactgcttt
    10021 ttcttgccgt tttcgtcggt aatcaccatt cccggcggga tagtctgcca gttcagttcg
    10081 ttgttcacac aaacggtgat acctgcacat caacaaattt tggtcatata ttagaaaagt
    10141 tataaattaa aatatacaca cttataaact acagaaaagc aattgctata tactacattc
    10201 ttttattttg aaaaaaatat ttgaaatatt atattactac taattaatga taattattat
    10261 atatatatca aaggtagaag cagaaactta cgtacacttt tcccggcaat aacatacggc
    10321 gtgacatcgg cttcaaatgg cgtatagccg ccctgatgct ccatcacttc ctgattattg
    10381 acccacactt tgccgtaatg agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc
    10441 caacctttcg gtataaagac ttcgcgctga taccagacgt tgcccgcata attacgaata
    10501 tctgcatcgg cgaactgatc gttaaaactg cctggcacag caattgcccg gctttcttgt
    10561 aacgcgcttt cccaccaacg ctgaccaatt ccacagtttt cgcgatccag actgaatgcc
    10621 cacaggccgt cgagtttttt gatttcacgg gttggggttt ctacaggacg taacataagg
    10681 gactgaccta cccggggatc ctctagagtc gaggtcctct ccaaatgaaa tgaacttcct
    10741 tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
    10801 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
    10861 atgttcctcg tgggtggggg tccatctttg ggaccactgt cggtagaggc atcttgaacg
    10921 atagcctttc ctttatcgca atgatggcat ttgtagaagc catcttcctt ttctactgtc
    10981 ctttcgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
    11041 ctttgttgaa aagtctcaat agccctctgg tcttctgaga ctgtatcttt gatattcttg
    11101 gagtagacga gagtgtcgtg ctccaccatg ttgacctgca ggcatgcaag cttgcatgcc
    11161 tgcaggtcga ctctagagga tccccgggta ccgagctcga attcgtaatc atgtcatagc
    11221 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    11281 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    11341 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    11401 gcgcggggag aggcggtttg cgtattggag cttgagcttg gatcagattg tcgtttcccg
    11461 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
    11521 gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
    11581 tttgtatgtg catgccaacc acagggttcc cctcgggatc aa
//
