LOCUS       sequence-259433-        3474 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0530
FEATURES             Location/Qualifiers
     source          1..3474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             212..232
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             515..2008
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     terminator      complement(2034..2068)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2139..2798)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2713..2732
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2980..3474,1..94)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(3469..3474,1..14)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
       61 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      121 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      181 tgtggataac cgtagtcggt ctcatatggc ccccaagaag aagaggaagg ttggtattca
      241 cgggggtacc cgcccatatg cttgccctgt cgagtcctgc gatcgccgct tttctcgctc
      301 ggatgagctt acccgccata tccgcatcca taccggtcag aagcccttcc agtgtcgaat
      361 ctgcatgcgt aacttcagtc gtagtgacca ccttaccacc cacatccgca cccacacagg
      421 cgagaagcct tttgcctgtg acatttgtgg gaggaagttt gccaggagtg atgaacgcaa
      481 gaggcatacc aaaatccata caggtgctag cttcatgaag atggacaaaa agactatagt
      541 ttggtttaga agagatctaa ggattgagga taatcctgca ttagcagcag ctgctcacga
      601 aggatctgtt tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg
      661 aagagcttca agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc
      721 tcttggatct gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg
      781 tatccgcgtt accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt
      841 agttcgggac cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta
      901 caatggagat ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac
      961 gagtttcaat tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc
     1021 tcctccttgg cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat
     1081 tgaagaacta gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc
     1141 ttggagtcca ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt
     1201 gatagattat gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc
     1261 gtatctccat ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca
     1321 aattatatgg gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag
     1381 gggaatcggt ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga
     1441 gcaatcgttg ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa
     1501 ggcctggaga caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg
     1561 ggctaccgga tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt
     1621 tcttctcctt ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga
     1681 tttggaatgt gacatccttg gctggcagta tatctctggg agtatccccg atggccacga
     1741 gcttgatcgc ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta
     1801 cataaggcaa tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg
     1861 ggacgctcct ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa
     1921 acccattgta gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg
     1981 tgaagcacag atcatgatcg gagcagcagc ccggggatcc tgagaccaga ccaataaaaa
     2041 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2101 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     2161 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     2221 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     2281 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     2341 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     2401 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     2461 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     2521 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     2581 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     2641 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     2701 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     2761 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     2821 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     2881 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     2941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3001 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3061 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3121 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3181 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3361 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3421 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaa
//
