LOCUS       sequence-259483-        9978 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector expressing WT TBK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Sarraf SA, Sideris DP, Giagtzoglou N, Ni L, Kankel MW, Sen A, 
            Bochicchio LE, Huang CH, Nussenzweig SC, Worley SH, Morton PD, 
            Artavanis-Tsakonas S, Youle RJ, Pickrell AM
  TITLE     PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged 
            Mitochondria, Inhibiting Mitosis.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):225-235.e5. doi: 
            10.1016/j.celrep.2019.08.085.
  PUBMED    31577952
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "225-235.e5"
COMMENT     Sequence Label: pHAGE-N-FLAG-HA-TBK1
FEATURES             Location/Qualifiers
     source          1..9978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        170..473
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        474..677
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     628..648
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     674..698
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             783..806
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             819..845
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    861..885
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3073..3097)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3110..3677
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3127..3679
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3271..3288)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3498..3517
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3691..4290
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3691..3710)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4187..4207
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4307..4895
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4360..4380)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4778..4789)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4970..5203
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5322..5340)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5408..5512
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5513..5581,5582..6373)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5731..5750)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6544..7132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7033..7052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7286..7303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7543..7564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7579..7584,7585..7602,7603..7609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7617..7633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7622..7644
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7641..7657
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7641..7657
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7707..7726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7918..8551
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8598..8723
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9220..9453
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9638..9682
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt ggagttccgc gttacataac
      181 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      421 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      481 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      541 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      601 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      661 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      721 gttttgacct ccatagaaga caccggcggc cgcagatctc tcgaggttga tctgccacca
      781 tggactacaa ggatgacgat gacaagctcg atggaggata cccatacgat gttccagatt
      841 acgctgctcg aggttatcaa acaagtttgt acaaaaaagt tggcatgcag agcacttcta
      901 atcatctgtg gcttttatct gatattttag gccaaggagc tactgcaaat gtctttcgtg
      961 gaagacataa gaaaactggt gatttatttg ctatcaaagt atttaataac ataagcttcc
     1021 ttcgtccagt ggatgttcaa atgagagaat ttgaagtgtt gaaaaaactc aatcacaaaa
     1081 atattgtcaa attatttgct attgaagagg agacaacaac aagacataaa gtacttatta
     1141 tggaattttg tccatgtggg agtttataca ctgttttaga agaaccttct aatgcctatg
     1201 gactaccaga atctgaattc ttaattgttt tgcgagatgt ggtgggtgga atgaatcatc
     1261 tacgagagaa tggtatagtg caccgtgata tcaagccagg aaatatcatg cgtgttatag
     1321 gggaagatgg acagtctgtg tacaaactca cagattttgg tgcagctaga gaattagaag
     1381 atgatgagca gtttgtttct ctgtatggca cagaagaata tttgcaccct gatatgtatg
     1441 agagagcagt gctaagaaaa gatcatcaga agaaatatgg agcaacagtt gatctttgga
     1501 gcattggggt aacattttac catgcagcta ctggatcact gccatttaga ccctttgaag
     1561 ggcctcgtag gaataaagaa gtgatgtata aaataattac aggaaagcct tctggtgcaa
     1621 tatctggagt acagaaagca gaaaatggac caattgactg gagtggagac atgcctgttt
     1681 cttgcagtct ttctcggggt cttcaggttc tacttacccc tgttcttgca aacatccttg
     1741 aagcagatca ggaaaagtgt tggggttttg accagttttt tgcagaaact agtgatatac
     1801 ttcaccgaat ggtaattcat gttttttcgc tacaacaaat gacagctcat aagatttata
     1861 tacatagcta taatactgct actatatttc atgaactggt atataaacaa accaaaatta
     1921 tttcttcaaa tcaagaactt atctacgaag ggcgacgctt agtcttagaa cctggaaggc
     1981 tggcacaaca tttccctaaa actactgagg aaaaccctat atttgtagta agccgggaac
     2041 ctctgaatac cataggatta atatatgaaa aaatttccct ccctaaagta catccacgtt
     2101 atgatttaga cggggatgct agcatggcta aggcaataac aggggttgtg tgttatgcct
     2161 gcagaattgc cagtacctta ctgctttatc aggaattaat gcgaaagggg atacgatggc
     2221 tgattgaatt aattaaagat gattacaatg aaactgttca caaaaagaca gaagttgtga
     2281 tcacattgga tttctgtatc agaaacattg aaaaaactgt gaaagtatat gaaaagttga
     2341 tgaagatcaa cctggaagcg gcagagttag gtgaaatttc agacatacac accaaattgt
     2401 tgagactttc cagttctcag ggaacaatag aaaccagtct tcaggatatc gacagcagat
     2461 tatctccagg tggatcactg gcagacgcat gggcacatca agaaggcact catccgaaag
     2521 acagaaatgt agaaaaacta caagtcctgt taaattgcat gacagagatt tactatcagt
     2581 tcaaaaaaga caaagcagaa cgtagattag cttataatga agaacaaatc cacaaatttg
     2641 ataagcaaaa actgtattac catgccacaa aagctatgac gcactttaca gatgaatgtg
     2701 ttaaaaagta tgaggcattt ttgaataagt cagaagaatg gataagaaag atgcttcatc
     2761 ttaggaaaca gttattatcg ctgactaatc agtgttttga tattgaagaa gaagtatcaa
     2821 aatatcaaga atatactaat gagttacaag aaactctgcc tcagaaaatg tttacagctt
     2881 ccagtggaat caaacatacc atgaccccaa tttatccaag ttctaacaca ttagtagaaa
     2941 tgactcttgg tatgaagaaa ttaaaggaag agatggaagg ggtggttaaa gaacttgctg
     3001 aaaataacca cattttagaa aggtttggct ctttaaccat ggatggtggc cttcgcaacg
     3061 ttgactgtct ttagccaact ttcttgtaca aagtggttcg ataacgaatt ccgccccccc
     3121 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     3181 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     3241 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     3301 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     3361 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     3421 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     3481 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     3541 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     3601 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     3661 gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac ggtgcgcctc
     3721 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     3781 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     3841 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     3901 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     3961 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     4021 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     4081 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     4141 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     4201 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     4261 tggtgcatga cccgcaagcc cggtgcctga tgcatcgatg gatcctaatc aacctctgga
     4321 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4381 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4441 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4501 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4561 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4621 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4681 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     4741 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4801 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4861 gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact
     4921 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     4981 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5041 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5101 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5161 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5221 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg
     5281 ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     5341 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5401 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5461 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5521 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5581 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5641 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5701 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     5761 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5821 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5881 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5941 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6001 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6061 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6121 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6181 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6241 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6301 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6361 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6421 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6481 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6661 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7141 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7201 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7261 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7321 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
     7381 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7441 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
     7501 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7561 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7621 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
     7681 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7741 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     7801 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     7861 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     7921 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     7981 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     8041 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     8101 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8161 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     8221 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     8281 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     8341 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8401 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8461 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8521 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8581 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8641 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8701 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8761 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8821 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8881 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8941 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9001 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9061 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9121 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9181 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9241 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9301 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9361 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9421 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9481 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9541 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9601 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9661 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9721 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9781 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9841 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     9901 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaatta attcacaaat
     9961 ggcagtattc atccacaa
//
