LOCUS       sequence-259528-       11204 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expresses MBP-tagged human Norrin ( 31–133) in Hi5 insect cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Shen G, Ke J, Wang Z, Cheng Z, Gu X, Wei Y, Melcher K, Xu HE, Xu W
  TITLE     Structural basis of the Norrin-Frizzled 4 interaction.
  JOURNAL   Cell Res. 2015 Sep;25(9):1078-81. doi: 10.1038/cr.2015.92. Epub 2015
            Jul 31.
  PUBMED    26227961
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2015.92"; journalName: "Cell Res"; date: "2015-09"; 
            volume: "25"; issue: "9"; pages: "1078-81"
COMMENT     Sequence Label: pAcGP67B MBP-tagged human Norrin ( 31–133)
FEATURES             Location/Qualifiers
     source          1..11204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1104
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKL
                     EEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGK
                     LIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLI
                     AADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKG
                     ETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEF
                     LENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSA
                     FWYAVRTAVINAASGRQTVDEALKDAQT"
     misc_recomb     1925..2630
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           2013..2385
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(2042..2630)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(3064..3081)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4298..4314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4298..4314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4311..4333)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4322..4338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4346..4352,4353..4370,4371..4376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4391..4412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4529..4546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4700..5288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4780..4799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5459..6250,6251..6319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6082..6101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6320..6424)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6492..6510
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6548..6570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6670..6689
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6883..6905
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6897..6914
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6898..6914
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     10087..10914
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             10087..10211
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(10249..10854)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        10918..11009
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     10934..10951
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     11106..11165
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             11181..11204
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atggcaaaaa tcgaagaagg taaactggta atctggatta acggcgataa aggctataac
       61 ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag
      121 catccggata aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac
      181 attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa
      241 atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt
      301 tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac
      361 aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa
      421 ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg
      481 ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt
      541 aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg
      601 attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat
      661 aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc
      721 aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc
      781 gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcgaaagag
      841 ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa agacaaaccg
      901 ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc acgtattgcc
      961 gccactatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca gatgtccgct
     1021 ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat
     1081 gaagccctga aagacgcgca gactaacgcg gcggccataa tggactcgga ccctcgacgc
     1141 tgcatgaggc accactatgt ggattctatc agtcacccat tgtacaagtg tagctcaaag
     1201 atggtgctcc tggccaggtg cgaggggcac tgcagccagg cgtcacgctc cgagcctttg
     1261 gtgtcgttca gcactgtcct caagcaaccc ttccgttcct cctgtcactg ctgccggccc
     1321 cagacttcca agctgaaggc actgcggctg cgatgctcag ggggcatgcg actcactgcc
     1381 acctaccggt acatcctctc ctgtcactgc gaggaatgca attcctgagc ggccgctgca
     1441 gatctgatcc tttcctggga cccggcaaga accaaaaact cactctcttc aaggaaatcc
     1501 gtaatgttaa acccgacacg atgaagcttg tcgttggatg gaaaggaaaa gagttctaca
     1561 gggaaacttg gacccgcttc atggaagaca gcttccccat tgttaacgac caagaagtga
     1621 tggatgtttt ccttgttgtc aacatgcgtc ccactagacc caaccgttgt tacaaattcc
     1681 tggcccaaca cgctctgcgt tgcgaccccg actatgtacc tcatgacgtg attaggatcg
     1741 tcgagccttc atgggtgggc agcaacaacg agtaccgcat cagcctggct aagaagggcg
     1801 gcggctgccc aataatgaac cttcactctg agtacaccaa ctcgttcgaa cagttcatcg
     1861 atcgtgtcat ctgggagaac ttctacaagc ccatcgttta catcggtacc gactctgctg
     1921 aagaggagga aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag
     1981 acgcacctct gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt
     2041 attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata
     2101 attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag
     2161 cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat
     2221 tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc
     2281 tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt
     2341 gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc
     2401 tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat aaagcttgga
     2461 catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct
     2521 cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg
     2581 tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt atttctgatt
     2641 gcgggcgttt ttgggcgggt ttcaatctaa ctgtgcccga ttttaattca gacaacacgt
     2701 tagaaagcga tggtgcaggc ggtggtaaca tttcagacgg caaatctact aatggcggcg
     2761 gtggtggagc tgatgataaa tctaccatcg gtggaggcgc aggcggggct ggcggcggag
     2821 gcggaggcgg aggtggtggc ggtgatgcag acggcggttt aggctcaaat gtctctttag
     2881 gcaacacagt cggcacctca actattgtac tggtttcggg cgccgttttt ggtttgaccg
     2941 gtctgagacg agtgcgattt ttttcgtttc taatagcttc caacaattgt tgtctgtcgt
     3001 ctaaaggtgc agcgggttga ggttccgtcg gcattggtgg agcgggcggc aattcagaca
     3061 tcgatggtgg tggtggtggt ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg
     3121 gcggtgccgc cggtataatt tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg
     3181 gcgcaggcgc cgctggctgc acaacggaag gtcgtctgct tcgaggcagc gcttggggtg
     3241 gtggcaattc aatattataa ttggaataca aatcgtaaaa atctgctata agcattgtaa
     3301 tttcgctatc gtttaccgtg ccgatattta acaaccgctc aatgtaagca attgtattgt
     3361 aaagagattg tctcaagctc gcacgccgat aacaagcctt ttcattttta ctacagcatt
     3421 gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt caaaaacgtc
     3481 gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg tgttgtcgta
     3541 aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa attgatgcgc
     3601 atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat atctacgatt
     3661 cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg aaaactgcaa
     3721 aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat ctattttatc
     3781 gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca attttaacgc
     3841 tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa aaatctattt
     3901 taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact gtcccgattt
     3961 atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca atattattag
     4021 acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca acgacataaa
     4081 actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg attacggatt
     4141 gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt ttagtccgga
     4201 aaaaattcga caccacaact atgcacgttc gtttgactgg tacgccgtcg gcgtgttaac
     4261 atacaagttg ctaaccggcg gccaattcgt aatcatggtc atagctgttt cctgtgtgaa
     4321 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4381 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4441 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4501 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4561 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4621 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5101 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6421 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     6481 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     6541 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6601 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     6661 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     6721 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     6781 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6841 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6901 aaacgacggc cagtgccaag cttggctcgt aaagcgagtt gaaggatcat atttagttgc
     6961 gtttatgaga taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt
     7021 tacaatgcgg ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc
     7081 ccgagttgtt tgcgtacgtg actagcgaag aagatatgtg gaccgcagaa cagatagtaa
     7141 aacaaaaccc tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg
     7201 gtgtgcattt tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt
     7261 tgccgcctga aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt
     7321 gtcccggcat gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg
     7381 tgtgcagata tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg
     7441 aaaaagccag aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat
     7501 taatattatt tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt
     7561 ccagcgaacc aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt
     7621 gttccaatcg acggtaggat taggccggat attctccacc acaatgttgg caacgttgat
     7681 gttacgttta tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt
     7741 agtgccgtcg cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac
     7801 cgcgcgatcc gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt
     7861 ctgcattatt tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag
     7921 atcagtcatg acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc
     7981 aacgatgcgt tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac
     8041 cgcttgcagc gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt
     8101 gctctcctcc tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg
     8161 aattacttct tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat
     8221 aatcagctga atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg
     8281 gtcgcccctt ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa
     8341 taacgatttg tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt
     8401 taaactgtta gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta
     8461 ccgcaggttg aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt
     8521 gatacacgtg tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt
     8581 ggtaagcaat aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg
     8641 cagacggcgc cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac
     8701 atcgcaaaag ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc
     8761 ttatttaata aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc
     8821 gagcagttcg ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac
     8881 cagcggcgtg ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg
     8941 cacgtcggcc tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt
     9001 gcgcatatct atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga
     9061 aattaaatca ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc
     9121 gtcgattaaa tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta
     9181 ttcccgagtc aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat
     9241 ttaatgcaac tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc
     9301 cgttgtcgca tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt
     9361 gcgacgcaac gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt
     9421 tttacgaagc gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga
     9481 ctacaaaatt accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt
     9541 agtcgaatca ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg
     9601 tgtggagtcc gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc
     9661 ccgatgattt tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg
     9721 ttttggtcaa aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta
     9781 atgaaattaa aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat
     9841 gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt
     9901 gtataaaaaa aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca
     9961 ggaagaggtt tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa
    10021 accgatgttt aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg
    10081 aagtcatgca tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa
    10141 tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt
    10201 gtaattattg aataataaaa caattataaa tgtcaaattt gttttttatt aacgatacaa
    10261 accaaacgca acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc
    10321 gctgaggtaa tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat
    10381 tttattttca cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc
    10441 atttttctcc tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta
    10501 tagagtaaat tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc
    10561 gctgcgcata gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg
    10621 tgttgcttta attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa
    10681 tatgttgccg gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac
    10741 cggattaaca taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc
    10801 cttttctata ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat
    10861 gagacgcaca aactaatatc acaaactgga aatgtctatc aatatatagt tgctgatatc
    10921 atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta
    10981 acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg
    11041 ggcgcggatc tatgctacta gtaaatcagt cacaccaagg cttcaataag gaacacacaa
    11101 gcaagatggt aagcgctatt gttttatatg tgcttttggc ggcggcggcg cattctgcct
    11161 ttgcggcgga tcttggatcc caccatcacc atcaccatca ccat
//
