LOCUS       sequence-25961-e         788 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Retroviral eGFP shRNA #2 (H1/TO promoter), 3'LTR insertion, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pQCXIN X2/pTER shEGFP#1 (w491-1)
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(176..200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagtcttgga ttgggatctt ataagttccc tatcagtgat agagatcccg aagcagcacg
       61 acttcttctt caagagagaa gaagtcgtgc tgcttctttt tggaaaagct tggtaccgag
      121 ctcggatcca ctagtccagt gtggtggaat tcgcggccgc actcgagata tctagaccca
      181 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagcttgc
      241 caaacctaca ggtggggtct ttcattcccc cctttttctg gagactaaat aaaatctttt
      301 attttatcta tggctcgtac tctataggct tcagctggtg atattgttga gtcaaaacta
      361 gagcctggac cactgatatc ctgtctttaa caaattggac taatcgataa gcttatctct
      421 acactggctc gatggagtca gcctagtgtc cctcggaaag ccttgaggtc cctttggagc
      481 agttctgcac tcggcctctg cagcgctgca gccctcgagt gggtggtgag cagctcgccg
      541 tcgcgcagca ggcgccagcg ctccatccac gcagtgacct caggcggaaa acgggaagac
      601 acactcatga gatgcctgca agcaattcgt tctgtatcag gcgcaggagc gtcccgtccg
      661 ggtcgactct agccgatccc gctcagaana actcgtcaag aaggcgatag aaggcgatgc
      721 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
      781 caagctct
//
