LOCUS       sequence-260262-        1198 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            C-terminal V5-miniTurbo for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-hcRed-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             163..846
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(848..872)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             874..915
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggcccngctg cggtgtgttt tgtgagtttt ttaggcacct tttgaatgta atcatttggg
       61 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
      121 gcttttttgt tagacgcaac tttgtacaaa aaagttggca ccatggtgag cggcctgctg
      181 aaggagagta tgcgcatcaa gatgtacatg gagggcaccg tgaacggcca ctacttcaag
      241 tgcgagggcg agggcgacgg caaccccttc gccggcaccc agagcatgag aatccacgtg
      301 accgagggcg cccccctgcc cttcgccttc gacatcctgg ccccctgctg cgagtacggc
      361 agcaggacct tcgtgcacca caccgccgag atccccgact tcttcaagca gagcttcccc
      421 gagggcttca cctgggagag aaccaccacc tacgaggacg gcggcatcct gaccgcccac
      481 caggacacca gcctggaggg caactgcctg atctacaagg tgaaggtgca cggcaccaac
      541 ttccccgccg acggccccgt gatgaagaac aagagcggcg gctgggagcc cagcaccgag
      601 gtggtgtacc ccgagaacgg cgtgctgtgc ggccggaacg tgatggccct gaaggtgggc
      661 gaccggcacc tgatctgcca ccactacacc agctaccgga gcaagaaggc cgtgcgcgcc
      721 ctgaccatgc ccggcttcca cttcaccgac atccggctcc agatgctgcg gaagaagaag
      781 gacgagtact tcgagctgta cgaggccagc gtggcccggt acagcgacct gcccgagaag
      841 gccaacttgc caactttctt gtacaaagtg ggcggcaagc ccatccccaa ccccctgctg
      901 ggcctggaca gcaccgctag catcccgctg ctgaacgcta aacagattct gggacagctg
      961 gacggcggga gcgtgcagtc ctgcctgtgg tcgactccac caatcagtac tgctggatcg
     1021 aatcggcgag ctgaagagtg gggatgctgc atgcagaata tcagcagcng ngagagcaga
     1081 ggaggaatgt tctctccttt gagctactgt actgantttg tttgcgctga ccgggacagc
     1141 agcatcgctg ggnngtcatc gatggtcatg ccganncctg caagctgggg agcgacag
//
