LOCUS       sequence-260346-        1137 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway destination clone of eGFP (as control) tagged with 
            N-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-eGFP_IDG-K
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(138..158)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             162..430
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             440..481
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    479..503
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      503..512
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(554..575)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(815..834)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggctggcga gtgtgttttg tgagtttttt aggcaccttt tgaaatgtaa tcatttgggt
       61 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
      121 cttttttgtt agacggccaa cttttgtata caaagtggcg aggggctcgc atctctcctt
      181 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
      241 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
      301 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
      361 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
      421 gttacagatc ggcaccatgg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
      481 aactttgtac aaaaaagttg gcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      541 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      601 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      661 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      721 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      781 aagctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      841 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      901 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacacag ccacacgtct
      961 atatcatggg ccgacagcag aagacggcat caaggtgaac ttcagatccg ccacaacatc
     1021 gaggacggca gcgtgcagct cgccgacact acagcagaac acccccatcg gcgacggctc
     1081 gtgctgctgc ccgacacact actggagcac cagtccgcct ggagcaagac ccaacga
//
