LOCUS       sequence-260364-        1216 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1216)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..1216
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(139..159)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             172..942
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKHGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             949..990
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 actggctgcg agtgtgtttt gtgagttttt taggcacctt ttgaaatgta atcatttggg
       61 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
      121 gcttttttgt tagacggcca acttttgtat acaaagtggg ccaccatggg aatcccgctg
      181 ctgaacgcta aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg
      241 gtcgactcca ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct
      301 tgcattgcag aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct
      361 tttggagcta acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc
      421 ggcctgggcc cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac
      481 aaggtgcgag tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc
      541 ctggtggagc tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt
      601 aacgtggcta tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag
      661 gaagcaggga ttaacctgga caggaatact ctggccgcta tgctgatccg agagctgcgg
      721 gcagccctgg aactgttcga gcaggaaggc ctggctccat atctgtcacg gtgggagaag
      781 ctggataact tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg
      841 attagccggg ggattgataa acacggagcc ctgctgctgg aacaggacgg agttatcaaa
      901 ccctggatgg gcggagaaat cagtctgcgg tctgccgaaa aggctagcgg caagcccatc
      961 cccaaccccc tgctgggcct ggacagcaca caactttgta caaaaagcag atggcccgtc
     1021 aaggcccacc atgacatcga cgggaagacg gcgcgcgcag cacgcgcagt atgttgggcc
     1081 ctacgcctga gaagacgctg ggcagggcag acagtctgtg agctggggtc actgcgtcac
     1141 ttgcaaagtg ncatcagatc gtcaacgtgg aaaagcttca ccgagtcggt gcctgattga
     1201 aggtgggagc gggnat
//
