LOCUS       sequence-260374-        1195 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            N-terminal Flag for generating protein-protein networks using fusion
            tag affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1195)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-hcRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..1195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(119..802)
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(805..829)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(839..862)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      862..871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(942..966)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(963..1014)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(992..1012)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccggcttgga gcgtctcacc ccaacagctg gccctcgcag acagcgaatt aattccagca
       61 cactggcggc cgttactagt ggatcttaat caaccacttt gtacaagaaa gttggcaagt
      121 tggccttctc gggcaggtcg ctgtaccggg ccacgctggc ctcgtacagc tcgaagtact
      181 cgtccttctt cttccgcagc atctggagcc ggatgtcggt gaagtggaag ccgggcatgg
      241 tcagggcgcg cacggccttc ttgctccggt agctggtgta gtggtggcag atcaggtgcc
      301 ggtcgcccac cttcagggcc atcacgttcc ggccgcacag cacgccgttc tcggggtaca
      361 ccacctcggt gctgggctcc cagccgccgc tcttgttctt catcacgggg ccgtcggcgg
      421 ggaagttggt gccgtgcacc ttcaccttgt agatcaggca gttgccctcc aggctggtgt
      481 cctggtgggc ggtcaggatg ccgccgtcct cgtaggtggt ggttctctcc caggtgaagc
      541 cctcggggaa gctctgcttg aagaagtcgg ggatctcggc ggtgtggtgc acgaaggtcc
      601 tgctgccgta ctcgcagcag ggggccagga tgtcgaaggc gaagggcagg ggggcgccct
      661 cggtcacgtg gattctcatg ctctgggtgc cggcgaaggg gttgccgtcg ccctcgccct
      721 cgcacttgaa gtagtggccg ttcacggtgc cctccatgta catcttgatg cgcatactct
      781 ccttcagcag gccgctcacc atggtgccaa cttttttgta caaacttgtt gatccgccct
      841 tgtcgtcgtc gtccttgtag tccatggtgg cctcgagctg accggtatgg ccgccggtgt
      901 cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa
      961 cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
     1021 ggcgggagtt gttacgacat ttttggaaag tcccgttgat tttggtgcca aacaaactcc
     1081 cattgacgtc atgggggtgg agacttgaaa tcccgtgagt caaacgctat tccacgccca
     1141 atgaatgtac tgcaaacgcc atcaccatgg taaaagcgga gtgaactaat acggt
//
