LOCUS       sequence-260802-        4165 bp    DNA     circular SYN 15-APR-2025
DEFINITION  pZS2-proD-soxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM11
FEATURES             Location/Qualifiers
     source          1..4165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      672..758
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1303..2253)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2301..2523
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3021..3115)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3146..3940)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3257..3276)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3867..3886
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4119..4137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaattc ttcacacagg aaaccggtac catggaaaag
      181 aaattacccc gcattaaagc gctgctaacc cccggcgaag tggcgaaacg cagcggtgtg
      241 gcggtatcgg cgctgcattt ctatgaaagt aaagggttga ttaccagtat ccgtaacagc
      301 ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg ttgcaattat caaaattgct
      361 cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt ttggcgtgtt gcccgaaggg
      421 catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc aatggcgaga agagttggat
      481 cggcgcattc ataccttagt ggcgctgcgt gacgaactgg acggatgtat tggttgtggc
      541 tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg accgcttagg agaagaaggt
      601 accggcgcac gcttgctgga agatgaacaa aactaacccg ggggatccca tggtacgcgt
      661 gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      721 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctagggcc
      781 tagggtacgg gttttgctgc ccgcaaacgg gctgttctgg tgttgctagt ttgttatcag
      841 aatcgcagat ccggcttcag ccggtttgcc ggctgaaagc gctatttctt ccagaattgc
      901 catgattttt tccccacggg aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg
      961 ataagcagca tcgcctgttt caggctgtct atgtgtgact gttgagctgt aacaagttgt
     1021 ctcaggtgtt caatttcatg ttctagttgc tttgttttac tggtttcacc tgttctatta
     1081 ggtgttacat gctgttcatc tgttacattg tcgatctgtt catggtgaac agctttgaat
     1141 gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg
     1201 catatggaca gttttccctt tgatatgtaa cggtgaacag ttgttctact tttgtttgtt
     1261 agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt
     1321 agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt
     1381 gagatcatac ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat
     1441 ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt aggaatctga
     1501 tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt
     1561 tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc
     1621 tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt
     1681 caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa
     1741 cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc
     1801 ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc
     1861 cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa
     1921 aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa
     1981 gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt
     2041 agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt attggttata
     2101 agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc
     2161 cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag
     2221 ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta
     2281 atcactatac caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt
     2341 tgtgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc
     2401 ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat
     2461 ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa
     2521 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc
     2581 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa
     2641 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg
     2701 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc
     2761 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct
     2821 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc
     2881 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc
     2941 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg
     3001 tccctagtac tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     3061 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3121 gagctctcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     3181 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     3241 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     3301 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     3361 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     3421 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     3481 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     3541 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     3601 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     3661 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     3721 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg
     3781 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     3841 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     3901 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     3961 tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc
     4021 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg
     4081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4141 ttcgtcttca cctcgagatg ctagc
//
