LOCUS       sequence-261017-        5403 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of circular permutant nSpyCas9 at amino acid position 
            1029 encoding truncated human cytosine deaminase, hAIDx, at the 
            C-terminus. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: 1029-AIDx-CP
FEATURES             Location/Qualifiers
     source          1..5403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4183..4203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4219..4239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4861..4908
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4909..4932
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4942..4998
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggtaccgcca ccatgatagg caaggcaacc gctaagtatt ttttttacag caacatcatg
       61 aacttcttta agaccgagat taccctggcc aacggtgaaa tccgtaaacg cccgctgatt
      121 gaaaccaacg gtgaaaccgg cgaaatcgtg tgggacaagg gccgtgattt tgcgaccgtg
      181 cgcaaagtgc tgtccatgcc gcaggtgaac attgtgaaga aaaccgaggt gcagaccggc
      241 ggtttcagca aggaaagcat tctgccgaaa cgtaacagcg acaagctgat cgcccgcaag
      301 aaagattggg acccgaagaa atacggcggt ttcgatagcc cgaccgtggc ttatagcgtg
      361 ctggtggtgg ccaaagtgga gaagggtaaa agcaagaaac tgaagagcgt gaaagaactg
      421 ctgggcatca ccattatgga gcgtagcagc ttcgaaaaga acccgatcga ctttctggag
      481 gcgaagggtt acaaggaagt gaagaaagat ctgatcatta agctgccgaa atacagcctg
      541 tttgagctgg aaaatggtcg taaacgtatg ctggccagcg ccggcgagct gcaaaagggc
      601 aacgaactgg cgctgccgag caaatatgtg aacttcctgt acctggccag ccactatgag
      661 aagctgaaag gtagcccgga ggacaacgaa cagaagcagc tgtttgtgga gcagcacaaa
      721 cactatctgg atgagatcat tgaacagatc agcgagttca gcaagcgtgt gattctggcg
      781 gacgctaacc tggataaagt gctgagcgcc tacaacaaac accgtgacaa gccgatccgc
      841 gagcaggcgg aaaacatcat tcacctgttc accctgacca acctgggtgc gccggccgcg
      901 ttcaagtact ttgacaccac cattgatcgt aaacgctata ccagcaccaa ggaggtgctg
      961 gatgctaccc tgatccacca gagcattacc ggcctgtacg aaacccgtat tgatctgagc
     1021 cagctgggcg gtgacggcgg tagcggcggt agcggcggta gcggcggtag cggcggtagc
     1081 ggcggtagcg gcggtgacaa gaaatatagc atcggtctgg cgattggcac caacagcgtg
     1141 ggttgggctg tgatcaccga tgagtacaag gtgccgagca agaagttcaa ggtgctgggc
     1201 aacaccgacc gccacagcat caagaaaaac ctgattggtg ccctgctgtt tgatagcggt
     1261 gaaaccgccg aggccacccg tctgaagcgc accgcccgtc gccgttacac ccgtcgtaaa
     1321 aaccgtatct gctacctgca agagattttc agcaacgaaa tggccaaagt ggacgatagc
     1381 ttctttcacc gcctggagga gagcttcctg gtggaggaag acaagaaaca cgagcgtcac
     1441 ccgatctttg gcaacattgt ggatgaggtg gcttaccacg aaaagtatcc gaccatctac
     1501 cacctgcgta agaaactggt ggatagcacc gacaaggccg atctgcgcct gatctatctg
     1561 gctctggccc acatgatcaa gttccgtggt cactttctga tcgagggcga cctgaacccg
     1621 gataacagcg atgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttt
     1681 gaggaaaacc cgattaacgc cagcggtgtg gacgcgaagg ctatcctgag cgcgcgtctg
     1741 agcaaaagcc gccgtctgga gaacctgatc gcgcagctgc cgggtgaaaa gaaaaacggt
     1801 ctgtttggca acctgattgc tctgagcctg ggcctgaccc cgaacttcaa aagcaacttt
     1861 gacctggcgg aggatgctaa gctgcaactg agcaaggata cctatgacga tgacctggac
     1921 aacctgctgg cgcagatcgg cgaccagtac gctgatctgt tcctggctgc caagaacctg
     1981 agcgatgcga tcctgctgag cgacattctg cgtgtgaaca ccgagattac caaggccccg
     2041 ctgagcgcga gcatgatcaa acgctatgat gaacaccacc aggacctgac cctgctgaaa
     2101 gctctggtgc gtcagcagct gccggagaag tacaaagaaa tcttctttga ccagagcaag
     2161 aacggttacg ccggctatat tgatggcggt gcgagccagg aggagttcta caagtttatc
     2221 aaaccgattc tggagaaaat ggacggcacc gaggaactgc tggtgaagct gaaccgtgaa
     2281 gatctgctgc gtaaacagcg caccttcgac aacggtagca tcccgcacca gattcacctg
     2341 ggtgagctgc acgccatcct gcgccgtcag gaagatttct acccgtttct gaaggacaac
     2401 cgtgagaaga tcgaaaaaat tctgaccttc cgcattccgt actatgtggg tccgctggct
     2461 cgtggcaaca gccgctttgc ctggatgacc cgcaagagcg aggaaaccat caccccgtgg
     2521 aacttcgagg aagtggtgga caaaggcgcc agcgcgcaga gcttcattga gcgtatgacc
     2581 aactttgata agaacctgcc gaacgaaaaa gtgctgccga agcacagcct gctgtacgag
     2641 tatttcaccg tgtacaacga actgaccaag gtgaaatatg tgaccgaggg tatgcgcaag
     2701 ccggccttcc tgagcggcga acagaagaaa gcgatcgtgg acctgctgtt taaaaccaac
     2761 cgtaaggtga ccgtgaagca gctgaaagag gattatttca agaaaattga atgctttgac
     2821 agcgtggaga tcagcggtgt ggaagatcgt tttaacgcga gcctgggcac ctaccacgac
     2881 ctgctgaaaa tcattaagga taaagacttc ctggataacg aggaaaacga ggacatcctg
     2941 gaagatattg tgctgaccct gaccctgttt gaggaccgtg aaatgatcga ggaacgcctg
     3001 aagacctatg cccacctgtt cgatgacaaa gtgatgaaac agctgaagcg ccgtcgctac
     3061 accggttggg gccgtctgag ccgcaaactg atcaacggta ttcgcgataa acagagcggc
     3121 aagaccatcc tggattttct gaagagcgac ggtttcgcga accgtaactt tatgcagctg
     3181 attcacgatg acagcctgac cttcaaagag gacatccaga aggcgcaggt gagcggtcag
     3241 ggcgatagcc tgcacgaaca tattgctaac ctggccggta gcccggccat caagaagggc
     3301 atcctgcaaa ccgtgaaggt ggtggacgag ctggtgaaag tgatgggtcg tcacaagccg
     3361 gaaaacatcg tgattgagat ggcccgcgaa aaccagacca cccagaaagg ccagaagaac
     3421 agccgtgagc gcatgaagcg tatcgaggaa ggcatcaagg aactgggcag ccagatcctg
     3481 aaggagcacc cggtggaaaa cacccagctg caaaacgaga aactgtacct gtactacctg
     3541 caaaacggtc gtgacatgta tgtggatcag gaactggaca ttaaccgcct gagcgattat
     3601 gacgtggatg cgatcgtgcc gcagagcttc ctgaaggatg acagcattga caacaaagtg
     3661 ctgacccgta gcgataaaaa ccgcggcaag agcgacaacg tgccgagcga ggaagtggtg
     3721 aagaaaatga aaaactactg gcgtcagctg ctgaacgcca aactgatcac ccagcgtaag
     3781 tttgacaacc tgaccaaagc tgagcgcggc ggtctgagcg aactggataa agccggtttc
     3841 attaagcgtc agctggtgga aacccgccag atcaccaagc acgtggcgca gattctggac
     3901 agccgcatga acaccaagta tgacgagaac gataaactga tccgtgaagt gaaagtgatc
     3961 accctgaaga gcaaactggt gagcgatttt cgtaaagact tccagtttta caaggtgcgc
     4021 gagatcaaca actaccacca cgctcacgac gcctatctga acgcggtggt gggcaccgcc
     4081 ctgatcaaga agtacccgaa gctggagagc gagttcgtgt acggcgacta caaggtgtat
     4141 gatgtgcgta agatgatcgc caaaagcgag caggaaattg gcccgaagaa aaagcgtaaa
     4201 gtgggtagca gcggcagccc gaaaaagaaa cgcaaagtgg gtagcgacgc tctggatgac
     4261 ttcgacctgg atatgctggg cagcgacgcc ctggatgact ttggcggtgg cagcatggat
     4321 agcctgctga tgaaccgtcg caaattcctg tatcagttta agaacgtgcg ttgggctaaa
     4381 ggccgtcgcg aaacctacct gtgctatgtg gtgaaacgtc gcgatagcgc caccagcttc
     4441 agcctggact ttggttacct gcgcaacaag aacggctgcc acgtggagct gctgttcctg
     4501 cgttacatta gcgactggga cctggaccca ggccgttgct atcgcgtgac ctggtttacc
     4561 agctggagcc cgtgctacga ttgcgctcgt catgtggcgg acttcctgcg tggtaaccca
     4621 aacctgagcc tgcgtatctt caccgcgcgc ctgtactttt gcgaggaccg taaggctgag
     4681 ccggaaggtc tgcgtcgcct gcaccgtgcg ggtgtgcaga tcgcgatcat gaccttcaaa
     4741 gattactttt attgctggaa caccttcgtg gagaaccacg aacgcacctt taaggcctgg
     4801 gagggtctgc acgaaaacag cgtgcgtctg agccgccagc tgcgccgcat cctgctgcca
     4861 aagcgtccgg cggctaccaa gaaagcgggc caggctaaga aaaagaaaga ctacaaagat
     4921 gacgatgaca agggtagcgg cgctaccaac ttcagcctgc tgaagcaggc gggcgacgtg
     4981 gaggaaaatc cgggcccgat ggctaaaccg ctgagccagg aggaaagcac cctgatcgag
     5041 cgcgctaccg ccaccattaa cagcatcccg attagcgaag actatagcgt ggccagcgcc
     5101 gcgctgagca gcgatggtcg tattttcacc ggcgtgaacg tgtaccactt taccggtggc
     5161 ccgtgcgccg agctggtggt gctgggcacc gccgccgccg ccgccgccgg caacctgacc
     5221 tgcatcgtgg cgattggtaa cgaaaatcgt ggcatcctga gcccgtgcgg ccgttgccgc
     5281 caggtgctgc tggacctgca tccgggcatc aaggcgattg tgaaggatag cgacggccaa
     5341 ccgaccgccg tgggtattcg tgaactgctg ccatccggct atgtttggga gggctaagga
     5401 tcc
//
