LOCUS       sequence-261187-        3810 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Human LC8-like protein, codon optimised for Sf9 expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, 
            Roberts AJ
  TITLE     Structure of the dynein-2 complex and its assembly with 
            intraflagellar transport trains.
  JOURNAL   Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. 
            doi: 10.1038/s41594-019-0286-y.
  PUBMED    31451806
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 
            10.1038/s41594-019-0286-y."
COMMENT     Sequence Label: LC8-like
FEATURES             Location/Qualifiers
     source          1..3810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      148..736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     637..656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  871..1094
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1161..1694)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1883..1888,1889..1905,1906..1911))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1982..2015)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2145..2162
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    3311..3432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3348..3367)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3402..3421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3603..3768
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
       61 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct
      841 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
      901 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
      961 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1021 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     1081 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     1141 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     1201 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     1261 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     1321 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     1381 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     1441 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     1501 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     1561 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     1621 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     1681 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1741 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1801 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1861 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1921 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc
     1981 cataacttcg tatagcatac attatacgaa gttatctgta actataacgg tcctaaggta
     2041 gcgacgttta aacatccacc atttggacct cacatagctc ttgttgaaga gctgtgctag
     2101 cacatgattc ggtaatagct ctacgttgcc gatggagata attaaaatga taaccatctc
     2161 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     2221 attccggatt attcataccg tcccaccatc gggcgcggat cctcaacatg gcagttaagg
     2281 ttgcagcagc atcagctttg tctttgtgtc atgccgtgag gcgctcttcc aaacaaccac
     2341 tctttaagga gctgctcaga agacctttgc agttcagcgt ggtgactaag tgtgatcaga
     2401 aagctgtgat caagaatgcc gacatgagcg aggagatgca gcaggactcc gttgagtgtg
     2461 ctgcacaggc tctggagaaa tacaacattg aagacactgc tgcccacatt aagaaggagt
     2521 tcgacaagaa ggacaccgac tttactgttg ctaagcagct cttgatgact ctcaacaaag
     2581 cagaagtgct ggagtgcttg cctgccgtct gtatggatct cttgctccct gtcaccgcac
     2641 agcgcaattc attgatcccc ttgggcatcc aaggttgtag acgcctgaga ctcctgagcc
     2701 agggatgcgg atctgaggaa cgcgctcccg ttccacgcgt cgctgcaccc ctcgacctgt
     2761 ccctgcatcc tgttccactg agcctgaaac gccaccagga gaaaagggag ggagagaaat
     2821 caagccagca ggctgctggc tccttcggcg acgcactggt tggcaacatc aggggagttc
     2881 tcgaccagag agcttcagcc ccttccctcg ctccatttct ggtcggtttg ggagaaggta
     2941 agggagccag gaagaaggaa ggcaagagag gaggcttttg ctgctccgtg ctggaggagg
     3001 agtgggccac ccctttctcc tctcctggta atagagatgt gccctccgac ggttccgtgc
     3061 acccattcag attcaggggt aaagtgggcg tgaaacaaca gcctcaaagc tggctcagcg
     3121 agatgtggtt gcgccaaaac actcgcggta tttcactgtg actagagcct gcagtctcga
     3181 caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt
     3241 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3301 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3361 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3421 caatgtatct tatcatgtct ggaaacatcc accatttaaa tggagttagg tttaaacgga
     3481 tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa ggaagctgag
     3541 ttggctgctg ccaccgctga gcaataacta tcataacccc tagggtatac ccatctaatt
     3601 ggaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag
     3661 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa
     3721 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacaac cggttgactt
     3781 gggtcaactg tcagaccaag tttactcata
//
