LOCUS       sequence-261288-        8909 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses HLA-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7128 pHAGE-P-CMVt-N-HA-GAW-HLA-A
FEATURES             Location/Qualifiers
     source          1..8909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      30..618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     519..538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     772..789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1029..1050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1065..1070,1071..1088,1089..1095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1103..1119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1108..1130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1127..1143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1127..1143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1193..1212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1404..2037
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2084..2209
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2706..2939
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3124..3168
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3461..3578
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3630..3933
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3934..4137
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4088..4108
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4139..4157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4160..4178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4188..4212
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4299..4308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4311..4337
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4353..4377
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5473..5497)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5516..6015
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5551..5574)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5948..5967
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6036..6635
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6036..6055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6532..6552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6702..7290
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6755..6775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7173..7184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7365..7598
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7717..7735)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7803..7907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7908..7976,7977..8768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8126..8145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
       61 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      781 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      841 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg
      901 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      961 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     1021 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     1081 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     1141 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1201 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1261 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1321 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1381 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1441 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     1501 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     1561 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     1621 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     1681 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     1741 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     1801 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     1861 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     1921 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     1981 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2041 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2101 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2161 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2221 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2281 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2341 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2401 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     2461 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     2521 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     2581 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     2641 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     2701 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     2761 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     2821 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     2881 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     2941 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3001 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3061 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3121 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3181 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3241 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3301 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3361 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     3421 cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     3481 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     3541 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     3601 gagatccagt ttggactagt ggagttccgc gttacataac ttacggtaaa tggcccgcct
     3661 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     3721 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3781 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3841 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3901 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3961 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4021 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4081 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     4141 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
     4201 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     4261 gaccgatcca gcctccggac tctagcgttt aaacgctagc caccatggcg tacccatacg
     4321 atgttccaga ttacgctgct cgaggttatc aaacaagttt gtacaaaaaa gttggcatgg
     4381 ccgtcatggc gccccgaacc ctcgtcctgc tactctcggg ggccctggcc ctgacccaga
     4441 cctgggcggg ctcccactcc atgaggtatt tctacacctc cgtgtcccgg cccggccgcg
     4501 gggagccccg cttcatcgcc gtgggctacg tggacgacac gcagttcgtg cggttcgaca
     4561 gcgacgccgc gagccagagg atggagccgc gggcgccgtg gatagagcag gaggggccgg
     4621 agtattggga ccggaacaca cggaatgtga aggcccactc acagactgac cgagagagcc
     4681 tgcggatcgc gctccgctac tacaaccaga gcgaggacgg ttctcacacc atccagagga
     4741 tgtatggctg cgacgtgggg ccggacgggc gcttcctccg cgggtaccag caggacgctt
     4801 acgacggcaa ggattacatc gccctgaacg aggacctgcg ctcttggacc gcggcggaca
     4861 tggcggctca gatcacccag cgcaagtggg agacggccca tgaggcggag cagtggagag
     4921 cctacctgga gggccggtgc gtggagtggc tccgcagata cctggagaac gggaaggaga
     4981 cgctgcagcg cacggacgcc cccaagacgc atatgactca ccacgctgtc tctgaccatg
     5041 aggccaccct gaggtgctgg gccctgagct tctaccctgc ggagatcaca ctgacctggc
     5101 agcgggatgg ggaggaccag acccaggaca cggagctcgt ggagaccagg cctgcagggg
     5161 atgggacctt ccagaagtgg gcgtctgtgg tggtgccttc tggacaggag cagagataca
     5221 cctgccatgt gcagcatgag ggtctgccca agcccctcac cctgagatgg gagccgtctt
     5281 cccagcccac catccccatc gtgggcatca ttgctggcct ggttctcttt ggagctgtga
     5341 tcgctggagc tgtggtcgct gctgtgatgt ggaggaggaa gagctcagat agaaaaggag
     5401 ggagctactc tcaggctgca agcagtgaca gtgcccaggg ctctgatatg tctctcacag
     5461 cttgtaaagt gtgcccaact ttcttgtaca aagtggttcg ataacgaatt ctaccgggta
     5521 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     5581 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     5641 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     5701 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     5761 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     5821 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     5881 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     5941 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     6001 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     6061 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     6121 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     6181 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6241 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6301 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6361 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6421 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6481 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6541 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6601 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     6661 cgaccgaaag gagcgcacga ccccatgcat cgatagatcc taatcaacct ctggattaca
     6721 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6781 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6841 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6901 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6961 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7021 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7081 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7141 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7201 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7261 gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa
     7321 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7381 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7441 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7501 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7561 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7621 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat
     7681 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     7741 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     8161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8881 aacgtgagtt ttcgttccac tgagcgtca
//
