LOCUS       sequence-261386-        4976 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild
            type HTT (wtHTT, 18 polyQ repeats) in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Pircs K, Petri R, Madsen S, Brattas PL, Vuono R, Ottosson DR, 
            St-Amour I, Hersbach BA, Matusiak-Bruckner M, Lundh SH, Petersen A, 
            Deglon N, Hebert SS, Parmar M, Barker RA, Jakobsson J
  TITLE     Huntingtin Aggregation Impairs Autophagy, Leading to Argonaute-2 
            Accumulation and Global MicroRNA Dysregulation.
  JOURNAL   Cell Rep. 2018 Aug 7;24(6):1397-1406. doi: 
            10.1016/j.celrep.2018.07.017.
  PUBMED    30089251
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.07"; journalName: "Cell Rep"; date: 
            "2018-08-7- 7"; volume: "24"; issue: "6"; pages: "1397-1406"
COMMENT     Sequence Label: pAAV-hSyn-Htt171-18Q-myc-WPRE
FEATURES             Location/Qualifiers
     source          1..4976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   34..163
                     /label=AAV2 ITR
     promoter        223..670
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             1229..1258
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1274..1291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    1313..1901
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1366..1386)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1784..1795)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    1949..2083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1986..2005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2040..2059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2389..2977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2469..2488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3148..3939,3940..4008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3771..3790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4009..4113)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4181..4199
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4237..4259)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4359..4378
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4445..4900)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4582..4603)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4794..4813
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gggggggggg gggggggggg ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       61 gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtggc caactccatc actaggggtt cctcagatcg ggctgcagga
      181 attggccgct ctagactcta gctgcagagg gacctgcgta tgagtgcaag tgggttttag
      241 gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa
      301 gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag
      361 gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc
      421 gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg
      481 tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc
      541 cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg
      601 cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct
      661 gagagcgcag tcgaattcaa gctgctagca aggatccacc ggtaccatgg cgaccctgga
      721 aaagctgatg aaggccttcg agtccctcaa gtccttccag cagcagcagc agcagcagca
      781 gcagcagcag cagcagcagc agcagcaaca gccgccaccg ccgccgccgc cgccgccgcc
      841 gccgccgcct cctcagcttc ctcagccgcc gccgcaggca cagccgctgc tgcctcagcc
      901 gcagccgccc ccgccgccgc ccccgccgcc acccggcccg gctgtggctg aggagccgct
      961 gcaccgacca aagaaagaac tttcagctac caagaaagac cgtgtgaatc attgtctgac
     1021 aatatgtgaa aacatagtgg cacagtctgt cagaaattct ccagaatttc agaaacttct
     1081 gggcatcgct atggaacttt ttctgctgtg cagtgatgac gcagagtcag atgtcaggat
     1141 ggtggctgac gaatgcctca acaaagttat caaagctttg atggattcta atcttccaag
     1201 gttacagctc gagtctagag ggcccttcga acaaaaactc atctcagaag aggatctgaa
     1261 tatgcatacc ggtcatcatc accatcacca ttgaactagt ggatccgtcg acaatcaacc
     1321 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     1381 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     1441 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     1501 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     1561 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     1621 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     1681 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     1741 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     1801 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     1861 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     1921 cagcttatcg ataccgtcga cttcgagcaa cttgtttatt gcagcttata atggttacaa
     1981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgtctagc atcgaagatc
     2101 ccccgatctg aggaacccct agtgatggag ttggccactc cctctctgca gcctgcatta
     2161 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2221 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2821 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3001 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3061 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3181 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3241 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3301 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3361 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3421 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3481 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3541 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3601 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3661 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3721 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3781 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3841 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3901 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3961 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4021 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4081 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4141 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4201 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4261 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4321 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4381 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     4441 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4501 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4561 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4621 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4681 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4741 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4801 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4861 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
     4921 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag gctgca
//
