LOCUS       sequence-261503-        8792 bp    DNA     circular SYN 14-APR-2025
DEFINITION  pLenti_CMV-mCherry-BghA_EF1alpha-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Xiang JS, Kaplan M, Dykstra P, Hinks M, McKeague M, Smolke CD
  TITLE     Massively parallel RNA device engineering in mammalian cells with 
            RNA-Seq.
  JOURNAL   Nat Commun. 2019 Sep 23;10(1):4327. doi: 10.1038/s41467-019-12334-y.
  PUBMED    31548547
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-23"; volume: "10(1):4327. doi"; pages: " 
            10.1038/s41467-019-12334-y"
COMMENT     Sequence Label: pCS4077
FEATURES             Location/Qualifiers
     source          1..8792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      675..810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    850..971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(861..880)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     915..934
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             1043..1276
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(1365..2066)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             2096..2364
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(2377..2588)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(2668..2687)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2859..3238
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3239..3442
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3393..3413
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        3487..3505
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3536..3545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3542..4252
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3955..3974
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4298..4315)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4304..4528
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4599..4623)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4657..4773
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5040..5084)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5269..5502
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5995..6120
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6167..6347
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6348..6574)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6602..6622)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6602..6620)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6641..6657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6641..6657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6654..6676)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6665..6681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6689..6695,6696..6713,6714..6719))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6734..6755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6872..6889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7043..7631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7123..7142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7802..8593,8594..8662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8425..8444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8663..8767)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtacgactc
      661 gacgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
      721 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
      781 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga tagctagagc
      841 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      901 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      961 ataaacaagt tcctctcact ctctgatatt catttctttg caagttataa atactgaata
     1021 ataagatgac atgaactact actgctagag attttccaca ctgactaaaa gggtctgagg
     1081 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     1141 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     1201 atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag
     1261 tgagctagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
     1321 ttgtaagtca ttggtcttaa aggtaccggg ccctcgagat gcattcaatt aagcttgtgc
     1381 cccagtttgc tagggaggtc gcagtatctg gccactgcca cctcgtgctg ctcgacgtag
     1441 gtctcgttgt tggcctcctt gattctttcc agtctgtagt ccacatagta gacgccaggc
     1501 atcttgaggt tcttagcggg tttcttggat ctatatgtgg tcttgatgtt tgcgatcaga
     1561 tggctcccgc ccacgagctt cagggccatg tcgtttctgc cttccaggcc gccgtcagcg
     1621 gggtacagcg tctcggtgaa ggcctcccag ccgagtgttt tcttctgcat cacagggccg
     1681 ttggatgtga agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg
     1741 ctggtgtcct gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac tctctcccat
     1801 gtgaagccct cagggaagga ctgcttgaag aagtcgggga tgccctgggt gtggttgatg
     1861 aaggtcttgc tgccgtagag gaagctagta gccaggatgt cgaaggcgaa ggggagaggg
     1921 ccgccctcga ccaccttgat tctcatggtc tgggtgccct cgtagggctt gccttcgccc
     1981 tcggatgtgc acttgaagtg atggttgtcc acggtgccct ccatgtacag cttcatgtgc
     2041 atgttctcct taatcagctc gctcatggtg gcgaatactc gagggtcaca gcttggatct
     2101 gtaacggcgc agaacagaaa acgaaacaaa gacgtagagt tgagcaagca gggtcaggca
     2161 aagcgtggag agccggctga gtctaggtag gctccaaggg agcgccggac agaggcccgg
     2221 tctcgacctg agctttaaac ttacctagac ggcggacgca gttcaggagg caccacaggc
     2281 gggaggcggc agaacgcgac tcaaccggcg tggatggcgg cctcaggtag ggcggcgggc
     2341 gcgtgaagga gagatgcgag cccctcgaag cttcagctgt gttctggcgg caaacccgtt
     2401 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     2461 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     2521 ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     2581 ctctgcccac tgacgggcac cggagcgatc gcagatcccc cgggaggtta ctcatagatc
     2641 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2701 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     2761 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     2821 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     2881 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     2941 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3001 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     3061 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3121 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     3181 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     3241 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     3301 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     3361 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     3421 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
     3481 cgaaattaat acgactcact atagggagac ccaagctggc tagcacgcta cggtcgccac
     3541 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     3601 agtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     3661 ccgcccctac gagggcaccc agaccgccaa gctgaaagtg accaagggtg gccccctgcc
     3721 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     3781 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     3841 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     3901 cggcgagttc atctacaaag tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     3961 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4021 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4081 tgaagtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggag cctacaacgt
     4141 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4201 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aactgatcat
     4261 aaggatccaa aaagaagcta agcaaataaa atctagacct cgactgtgcc ttctagttgc
     4321 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4381 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4441 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4501 catgctgggg atgcggtggg ctctatggcc gcggtaccgt cgactgcaga attcgaagct
     4561 tgagctcgag atccagtcga ctgaattggt tcctttaaag cctgcttttt tgtacaaact
     4621 tgttgatatc tgcagaattc caccacactg gacgataaaa ttttgaattt tgtaatttgt
     4681 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
     4741 ctgtaccccc caatcccccc ttttctttta aaagttaacc gataccgtcg agatccgttc
     4801 actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc
     4861 ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
     4921 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
     4981 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
     5041 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
     5101 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
     5161 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
     5221 ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga
     5281 tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact
     5341 gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc
     5401 actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata
     5461 gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt
     5521 tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt
     5581 actactttat atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt
     5641 ctgaagatca gcggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat
     5701 cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt
     5761 tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag
     5821 ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa
     5881 tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc
     5941 tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct
     6001 ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag
     6061 tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag
     6121 ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc
     6181 cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca
     6241 cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt
     6301 tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccgtt tatgtatcga
     6361 gctaggcact taaatacaat atctctgcaa tgcggcaatt cagtggttcg tccaatccat
     6421 gtcagacccg tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat
     6481 cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg
     6541 ttgcaagact acaagagtat tgcataagac tacattaagc ttgcagctcc agcttttgtt
     6601 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6661 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6721 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6781 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6841 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6901 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6961 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7021 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7081 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7141 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7201 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7261 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7321 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7381 caccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7441 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7501 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7621 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7681 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7741 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7801 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7861 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7921 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7981 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8041 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8101 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8161 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8221 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8281 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8341 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8401 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8461 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8521 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8581 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8641 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8701 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8761 ttccgcgcac atttccccga aaagtgccac ct
//
