LOCUS       sequence-261541-        9927 bp    DNA     circular SYN 15-APR-2025
DEFINITION  To test the effect of sequence on nuclear body formation and 
            splicing of full-length TDP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1415 GFP-TDP43 VLIM-S
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2218..2242
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2332..2341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4300..4324)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4360..4948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4413..4433)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4831..4842)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4962..5461
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4997..5020)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5394..5413
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5482..6081
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5482..5501)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5978..5998
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6219..6452
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6524..6645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6561..6580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6615..6634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6685..6820
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6840..6859)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6841..6859)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6869..6886)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6869..6885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6878..6900)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7027..7482
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7114..7133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7324..7345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7508..7612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7613..7681,7682..8473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7831..7850)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8644..9232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9133..9152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9386..9403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9520..9541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9556..9561,9562..9579,9580..9586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9594..9610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9599..9621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9618..9634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9618..9634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9653..9673
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9655..9673
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9701..9927
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2281 aagacaccga ctctagtcca gtgtggtgga attctgcaga ttaccaccgg tgccaccatg
     2341 ggaagtaaag gagaggaact atttacaggt actgtgccta tcaaagtcga gctggacggc
     2401 gacgtaaacg gccacaagtt cagcgtgcgt ggcgagggcg agggcgatgc caccgagggc
     2461 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc cggtgccgtg gccgaccctc
     2521 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct acccggacca catgaagcgt
     2581 cacgacttct tcaagtccgc catgccggaa ggctacgtcc aggagcgcac catcgagttc
     2641 aaggacgacg gcacctacaa gacccgcgcg gaggtgaagt tcgagggcga caccctggtg
     2701 aaccgcatcg agctgaaggg caacgacttc aaggaggacg gcaacatcct gggtcacaag
     2761 ctggagtaca accacaacag ccacaacgtc cgtatcgaag ccgacaagca gaagaacggc
     2821 atcaaggcta acttcaagat ccgccacaac gttgaggacg gcagccagca ggaagccgac
     2881 cacaaacagc agaacacccc gatcggcgac ggcccggtgc gcctgccgga caaccactat
     2941 ctgagcaccc agaccacgct gagcaaagac ccgaacgaga agcgcgatca catggtcctg
     3001 aaggagttcg tgacggcagc cggtatcact aaaggtgaag atgaaagtgg atccatgtct
     3061 gaatatattc gggtaaccga agatgagaac gatgagccca ttgaaatacc atcggaagac
     3121 gatgggacgg tgctgctctc cacggttaca gcccagtttc caggggcgtg tgggcttcgc
     3181 tacaggaatc cagtgtctca gtgtatgaga ggtgtccggc tggtagaagg aattctgcat
     3241 gccccagatg ctggctgggg aaatctggtg tatgttgtca actatccaaa agataacaaa
     3301 agaaaaatgg atgagacaga tgcttcatca gcagtgaaag tgaaaagagc agtccagaaa
     3361 acatccgatt taatagtgtt gggtctccca tggaaaacaa ccgaacagga cctgaaagag
     3421 tattttagta cctttggaga agttcttatg gtgcaggtca agaaagatct taagactggt
     3481 cattcaaagg ggtttggctt tgttcgtttt acggaatatg aaacacaagt gaaagtaatg
     3541 tcacagcgac atatgataga tggacgatgg tgtgactgca aacttcctaa ttctaagcaa
     3601 agccaagatg agcctttgag aagcagaaaa gtgtttgtgg ggcgctgtac agaggacatg
     3661 actgaggatg agctgcggga gttcttctct cagtacgggg atgtgatgga tgtcttcatc
     3721 cccaagccat tcagggcctt tgcctttgtt acatttgcag atgatcagat tgcgcagtct
     3781 ctttgtggag aggacttgat cattaaagga atcagcgttc atatatccaa tgccgaacct
     3841 aagcacaaca gtaatcgaca gtccgagaga agcgggcggt tcggtggtaa tccagggggg
     3901 ttcggaaacc aaggaggttt tggtaactct aggggaggag gcgctggttc cggtaataac
     3961 caaggcagca acagtggggg gggcagtaat ttcggtgctt tttcaagtaa cccagcaatg
     4021 atggccgccg cacaagcagc tttgcaaagt tcttggggca tgatgggcat gctggcgtca
     4081 cagcaaaatc aaagcggacc ctcaggaaat aatcaaaacc aaggcaatag ccagagggag
     4141 cctaatcagg ccttcggatc aggcaataat tcatacagtg gttcaaactc cggggcagcc
     4201 tctgggtggg gcagcgcaag taatgccgga agcggtagtg gattcaacgg agggttcggg
     4261 tcaagtagcg atagtaaaag ttccggttgg ggctcttaga cccagctttc ttgtacaaag
     4321 tggttgatcc tctatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa
     4381 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4441 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4501 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4561 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4621 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4681 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4741 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     4801 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4861 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4921 cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt
     4981 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     5041 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     5101 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     5161 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     5221 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     5281 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     5341 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     5401 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     5461 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5521 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5581 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5641 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5701 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5761 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5821 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5881 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5941 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6001 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6061 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     6121 gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc
     6181 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca
     6241 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6301 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6361 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6421 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6481 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6541 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6601 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     6661 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6721 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6781 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     6841 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     6901 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6961 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7021 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7081 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7141 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7201 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7261 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7321 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7381 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7441 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     7501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7621 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7681 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7741 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7801 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7861 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7921 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7981 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8041 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8101 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8161 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8221 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8281 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8341 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8401 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8461 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8521 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8761 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9061 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     9661 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     9721 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     9781 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     9841 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     9901 tttaagtgcc tagctcgata cataaac
//
