LOCUS       sequence-261563-       11989 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2-VN (G112P)
FEATURES             Location/Qualifiers
     source          1..11989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1138..1140,1141..1599)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(1180..1201)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1441..1460)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    1622..1756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1646..1665)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1700..1719
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1898..1920)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2506..4295
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4601..6526)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6961..6979)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7047..7151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7152..7220,7221..8012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7370..7389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8183..8771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8672..8691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9388..9533
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9573..10598
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9573..10598
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10619..10638)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10663..10711
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11364..11743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11744..11947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11898..11918
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11944..11968
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
       61 gagcagaaat tgatcagcga ggaggacctg ggcagccctg gaggcagttc aggaggcgga
      121 tctcactcca tgaggtattt cttcacatcc gtgtcccggc ccggccgcgg ggagccccgc
      181 ttcatcgcag tgggctacgt ggacgacacg cagttcgtgc ggttcgacag cgacgccgcg
      241 agccagagga tggagccgcg ggcgccgtgg atagagcagg agggtccgga gtattgggac
      301 ggggagacac ggaaagtgaa ggcccactca cagactcacc gagtggacct ggggaccctg
      361 cgcggctact acaaccagag cgaggccggt tctcacaccg tccagaggat gtatggctgc
      421 gacgtggggt cggactggcg cttcctccgc ccttaccacc agtacgccta cgacggcaag
      481 gattacatcg ccctgaaaga ggacctgcgc tcttggaccg cggcggacat ggcagctcag
      541 accaccaagc acaagtggga ggcggcccat gtggcggagc agttgagagc ctacctggag
      601 ggcacgtgcg tggagtggct ccgcagatac ctggagaacg ggaaggagac gctgcagcgt
      661 acggacgccc ccaaaacgca tatgactcac cacgctgtct ctgaccatga agccaccctg
      721 aggtgctggg ccctgagctt ctaccctgcg gagatcacac tgacctggca gcgggatggg
      781 gaggaccaga cccaggacac ggagctcgtg gagaccaggc ctgcagggga tggaaccttc
      841 cagaagtggg cggctgtggt ggtgccttct ggacaggagc agagatacac ctgccatgtg
      901 cagcatgagg gtttgcccaa gcccctcacc ctgagatggg agccgtcttc ccagcccacc
      961 atccccatcg tgggcatcat tgctggcctg gttctctttg gagctgtgat cactggagct
     1021 gtggtcgctg ctgtgatgtg gaggaggaag agctcagata gaaaaggagg gagctactct
     1081 caggctgcaa gcagtgacag tgcccagggc tctgatgtgt ctctcacagc ttgtaaagtg
     1141 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1201 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1261 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1321 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1381 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1441 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1501 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1561 gagtacaact acaacagcca caacgtctat ctgaccgcct aactcgaggc cggcaaggcc
     1621 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1681 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1741 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     1801 ggtggggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     1861 ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     1921 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     1981 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccgg tcgactctag agccccgccg
     2041 ccggacgaac taaacctgac tacggcatct ctgccccttc ttcgctggta cgaggagcgc
     2101 ttttgttttg tattcggggc agtgcatgta atcccttcag ttggttggta caacttgcca
     2161 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact
     2221 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct
     2281 ggagcagact ttgcatgctg tggactgcaa cacaacattg cctttatgtg taactcttgg
     2341 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg
     2401 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa
     2461 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg
     2521 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc
     2581 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat
     2641 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag
     2701 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc
     2761 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg
     2821 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat
     2881 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca
     2941 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata
     3001 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg
     3061 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca
     3121 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag
     3181 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg
     3241 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt
     3301 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata
     3361 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc
     3421 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata
     3481 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
     3541 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
     3601 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     3661 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     3721 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     3781 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     3841 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
     3901 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     3961 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     4021 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     4081 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4141 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag
     4201 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa
     4261 gggggcgtga attttcgctg cttgtccttt tcctgcatgc tggttgctcc cattcttagg
     4321 tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc
     4381 gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg
     4441 gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga tggccagaaa
     4501 tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg gggaatacac
     4561 ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc ccttcctcac
     4621 cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc gcggcagccc
     4681 cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag ctgcacacag
     4741 tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta atcgcatcct
     4801 tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag acaatggact
     4861 cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg agacgactca
     4921 atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg
     4981 aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg tcggtagtcc
     5041 tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg
     5101 ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg cgcctgcctc
     5161 catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct gcggggccct
     5221 gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct tggtggtatt
     5281 caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga gggggcctgc
     5341 gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc ttttctccac
     5401 gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt tcacgtcctc
     5461 taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct cgaccccggc
     5521 ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct gctcctgccc
     5581 ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc
     5641 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     5701 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     5761 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc
     5821 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     5881 ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct cctgctcctg
     5941 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
     6001 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc
     6061 ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     6121 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc
     6181 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc
     6241 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
     6301 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
     6361 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
     6421 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
     6481 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
     6541 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
     6601 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
     6661 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
     6721 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
     6781 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
     6841 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
     6901 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
     6961 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7021 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7381 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7681 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7861 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     8341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctgcgccg
     8821 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     8881 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     8941 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     9001 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
     9061 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg
     9121 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     9181 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     9241 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
     9301 atgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa
     9361 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg
     9421 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt
     9481 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc
     9541 aacagcgtgc cgcagatccc gggcaatgag atatgaaaaa gcctgaactc accgcgacgt
     9601 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     9661 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     9721 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     9781 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     9841 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     9901 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     9961 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
    10021 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
    10081 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
    10141 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
    10201 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
    10261 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
    10321 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
    10381 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
    10441 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
    10501 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
    10561 accgacgccc cagcactcgt ccgagggcaa aggaataggg gagatggggg aggctaactg
    10621 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
    10681 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
    10741 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
    10801 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
    10861 gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg
    10921 gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtctggtc cacgactgga
    10981 ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg
    11041 acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg
    11101 gatttctggc gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
    11161 tccgggcaac gttgttgcat tgctgcaggc gcagaactgg taggtatgga agatctatac
    11221 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat
    11281 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
    11341 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
    11401 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    11461 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    11521 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    11581 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
    11641 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
    11701 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    11761 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    11821 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    11881 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    11941 cagagctcgt ttagtgaacc gtcagatctc tagaagctgg gtaccagct
//
