LOCUS       sequence-26315-e         662 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag GCK
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..79
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    134..158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttctctagg cgccggccgg atccagaatt cgccaccatg gggtcttcaa aatctaaacc
       61 aaaggacccc agccagcgcc ggcgcaggat ccgaggttac cttgactaca aagacgatga
      121 cgacaagcaa ttgacaagtt tgtacaaaaa agttggcatg ctggacgaca gagccaggat
      181 ggaggccgcc aagaaggaga aggtagagca gatcctggca gagttccagc tgcaggagga
      241 ggacctgaag aaggtgatga gacggatgca gaaggagatg gaccgcggcc tgaggctgga
      301 gacccatgaa gaggccagtg tgaagatgct gcccacctac gtgcgctcca ccccagaagg
      361 ctcagaagtc ggggacttcc tctccctgga cctgggtggc actaacttca gggtgatgct
      421 ggtgaaggtg ggagaaggtg aggaggggca gtggagcgtg aagaccaaac accagatgta
      481 ctccatcccc gaggacgcca tgaccggcac tgctgagatg ctcttcgact acatctctga
      541 gtgcatctcc gacttcctgg acaagcatca gatgaaacac aagaagctgc ccctgggctt
      601 caccttctcc tttcctgtga ggcacgaaga catcgataag ggcatccttc tcaactggac
      661 ca
//
