LOCUS       sequence-263467-        3921 bp    DNA     circular SYN 15-APR-2025
DEFINITION  full-length of gata2a without stop codon in pmE Gateway  vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S,
            Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND
  TITLE     Valves Are a Conserved Feature of the Zebrafish Lymphatic System.
  JOURNAL   Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 
            10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26.
  PUBMED    31564611
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.08.019"; journalName: "Dev Cell"; date: 
            "2019-11-4- 4"; volume: "51"; issue: "3"; pages: "374-386.e5"
COMMENT     Sequence Label: pME-gata2a
FEATURES             Location/Qualifiers
     source          1..3921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(129..156)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(248..334)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     383..405
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     397..414
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     398..414
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    430..529
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      532..541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1907..2006)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2023..2042)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2024..2042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2047..2063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2047..2063)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2165..2184)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2176..2985
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2243..2262)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3132..3720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3621..3640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3874..3891
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
       61 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
      121 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
      181 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
      241 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
      301 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
      361 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
      421 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
      481 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgccaccatg
      541 gaggttgcgg ccgatcagtc tcgatggatg gcccaccatc atgcggtact caatggccag
      601 cacccagagt cacatcatca cgggcttaca cacaattaca tggagcccat ggcccctctt
      661 ctacccccgg acgaggtgga tgtgttcttg aatcacttgg actcgcaggg gaacccctac
      721 tactccaact ctcgggctag agtatcgtac ggacaagcgc acgctcgtct cacgggaagt
      781 caggtctgtc ggccacacct catacacagt cccggtattc cctggctgga cagcgggaag
      841 gcagcgctct ccgccgccca ccacaacgcc tgggcggtca gccacttcag caagcccggc
      901 ctgcaccccg ccagcgccgc ttatccgtgc agcagcagtt caagcacggc tccagtttcc
      961 tccctcacat ccgcaaccca ctccagtccg catccccttt ataatttccc cccaacccca
     1021 ccgaaagacg tatcaccaga ccccggtcct tcttctccga catccaccac tgcaagaatg
     1081 gacgaaaagg agtccatcaa gtaccaagtg tccatcgcag atggcatgaa aatggaggga
     1141 tgtagtcctc ttcggggaag cttggctatg agtgcacaga caccctccac acatcatccc
     1201 atcccgacct acccaaccta ctccctgccc gcgccacacg actacggcgg cggcctgttt
     1261 catcccggta ccctcctcag cggatccgct tccagcttca cacctaaatg taaaagcaag
     1321 acgcgatcgt gctcagaggg gcgtgaatgt gtgaactgtg gagccacctc caccccgctg
     1381 tggcggcggg atggaacggg ccactacctc tgcaacgcgt gcggcctgta ccacaagatg
     1441 aacggccaga acagacccct tatcaagcca aagcgcagac tgtctgcggc gaggcgagct
     1501 ggcacctgct gcgcgaactg ccagacaacc acgaccaccc tgtggcggcg caacgggaac
     1561 ggtgacccgg tgtgcaacgc ctgcgggctc tactacaaac tccacaacgt caacaggcca
     1621 ctgaccatga agaaggaggg catccaaaca cgcaaccgca agatgtccag caagtccaag
     1681 aggagtaaac gatctggtga gggtttcgag gagctctcca agtgcatgca ggacaagacc
     1741 tctccgtttg gcagcgccag cgccctggca agccacatgc cacacatggg ccacctgcca
     1801 ccgttcagcc attccggaca catgctgccc acgccgaccc ccattcaccc cacattcagc
     1861 cacccgcacc attccggccg ctctcccgcc tgggccgaac cacaccaccc agctttcttg
     1921 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1981 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     2041 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     2101 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2161 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2221 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2281 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2341 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2401 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2461 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2521 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2581 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2641 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2701 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2761 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2821 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2881 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2941 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3001 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3061 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3901 gagcgcccaa tacgcaaacc g
//
