LOCUS       sequence-263626-        9800 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of nucleo-cytoplasmic mTagBFP2 in M-cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9800)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 9800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0763
FEATURES             Location/Qualifiers
     source          1..9800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3292..3309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3293..3309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3319..3337
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3761..3772
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5134..5841
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     terminator      5865..6052
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5920..5944)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6127..7483
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6127..6470
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6471..7280
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6538..6557)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7286..7483
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7540..7560)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7540..7558)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7579..7595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7579..7595)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7592..7614)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7603..7619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7627..7633,7634..7651,7652..7657))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7672..7693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7810..7827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7981..8569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8061..8080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8740..9531,9532..9600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9363..9382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9601..9705)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9773..9791
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccgct
     3361 agcatgcgag ctttgcgtaa ttatgtacgg cgtcctggga tagtcgctgt ttgttaaaaa
     3421 aaaggtgatt cgcaactgat gtatgcagat ggcgatgaga aagtagagag aggtagtgaa
     3481 aaaagataat gaaatataga gaactacgta acgctccaca atagaacaaa taccgtgcac
     3541 taaagtttca agaaatttcc gcttcaatag gaaagaataa acaaattaga atggtttgtt
     3601 ggtacgggag aaagccgatt acgaacagtc aaggcaatga ttttgattga tatgatcaac
     3661 caattttgat tttactaatt caaaatgaaa tcctattaat ttcaatcgat agctcgcccc
     3721 ttttacttac aattttttga aagatgttgt tgggggatga aaagaggaga aatcgaaagc
     3781 aaacaactgg cacaatatag ccgcgaaaaa aaattttttt aatgtaaata taccgcgtta
     3841 cttgaccttc ctttataagt aaatttttag tcctgtgatc ttccgaggca gcatgtgccg
     3901 tttgcataat ttatgaccaa atccgttacc cacatatatc atttgtttgc cttgagcacc
     3961 ccggataaaa ggttgaagag ttggtttccc gatatatggt ccttacacta aagtaaagga
     4021 tgctcagtaa gcttctcccg tctagactgg aaatagcgca aaaatcattt ggtatgtata
     4081 atcaaatatt acggaacgga gtacgactta aattaattct attaacaaat tgaaagcgca
     4141 tagctctttc agatatgaaa gtgaagcaga aggtgagcat tattcgcact tagcaacaga
     4201 aaatttgtaa ctgacgacct tgcaaaggct tgtaaggtga gctaagttcc gcttccgtct
     4261 acggttctta ccgttcttct cctcgtttcc gtgcgatgcg gtaccgtgta aaggcatacc
     4321 ccgtcttttt ctataactat tttaacgaaa cttgttgaag cttaatcctc acgagttttc
     4381 cgtttgggta tttcgtgtgg tttcggatgt ttcaaaagtt tggggtaatc gacatttgtc
     4441 cgttccccag atttctgaag ataatgttaa ggcttaccga atcaataacc tgacgtgttc
     4501 attcgtacat gtgccattgt tgttgacaaa gaattaaccc catttacaag tatggtgcgg
     4561 acaaccaatt actatatttt taccaaggtg gtcctccaac aaacgaatcc gttcaacgac
     4621 ttgggtcacc ttatataggt tgatttgcaa gaagttttgc ttattccatc tttggacggt
     4681 tgtagcacat aagatgtcca tgttagtacc caaataaagt attttgtttt agtttagata
     4741 attatataaa attaaaacta acttatccaa cttaagcgtg tcagcaatgc aatcaagaac
     4801 tgctattatt tatttacacc ttgacgagta caccacttgt cattcatttt cgtaactttc
     4861 gggagctact ttgaggttgt tcctttcatt cttttttttt tataaattta gttcctttgt
     4921 tatattttat tttattgtaa tctaacttct gcatcttcta tcaaagggat atttcccatt
     4981 gtgctgagta cacaatgggc ctttgttagg tactctccat tgttgtccac catacaaaat
     5041 gaagggcttg tcctctcttc agacaccatt cggtgcggtt tctttggcaa agaatcactc
     5101 tttggatttt gttttttaga attcatggca agcgtgtcta agggcgaaga gctgattaag
     5161 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     5221 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     5281 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     5341 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     5401 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     5461 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     5521 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
     5581 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     5641 gggagccatc tgatcgcaaa cgccaagacc acatatagat ccaagaaacc cgctaagaac
     5701 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     5761 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
     5821 aaactggggc acaagcttaa ttaaggcgcg ccacttctaa ataagcgaat ttcttatgat
     5881 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
     5941 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
     6001 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
     6061 ctagggataa cagggtaata tagatctgtt tagcttgcct cgtccccgcc gggtcacccg
     6121 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg
     6181 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca
     6241 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc
     6301 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg
     6361 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt
     6421 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgggtaagg
     6481 aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     6541 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     6601 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     6661 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     6721 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag
     6781 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     6841 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     6901 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     6961 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
     7021 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     7081 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     7141 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     7201 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     7261 tgctcgatga gtttttctaa tcagtactga caataaaaag attcttgttt tcaagaactt
     7321 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     7381 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     7441 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta
     7501 acgccgccat ccagtttaaa cgagctccag cttttgttcc ctttagtgag ggttaattgc
     7561 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9721 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9781 tatcacgagg ccctttcgtc
//
