LOCUS       sequence-263648-        9814 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of 3mCherry tagged CRIB domain to monitor active Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0756
FEATURES             Location/Qualifiers
     source          1..9814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1178..1828
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     rep_origin      complement(2262..2717)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2399..2420)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2611..2630
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2843..2865
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2857..2874
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2858..2874
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2884..2903
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2884..2902
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6558..6901
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      7300..7497
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7554..7574)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7554..7572)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7593..7609)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7593..7609)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7606..7628)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7617..7633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7641..7647,7648..7665,7666..7671))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7686..7707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7824..7841)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7995..8583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8075..8094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8754..9545,9546..9614))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9377..9396
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9615..9719)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9787..9805
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggatggaacg attgaatact gtgctactct ttatgtaata taacgaattc
      241 aactgttcaa cgtatgtgac tgctctctgc ttctcggtat atatttactt gaaggaatta
      301 atttttcttt gccaatggtc tcttttacca attgcagtct ggcttaaagc aatgaggttg
      361 taattaaatg gcttgagacc tagaacaaac accttttaca atccttgttt actggttttg
      421 atagtaaatt cggttcgctt gtggtgtaca ttatattttt taggctagtc gtgatttttt
      481 gataatatca atattcttag atttgagaca ttcatgcttt ttaatttgat caatcgtttg
      541 tatcttcatt aattagtagg tggtttgata gaacatttag ttgcttgttt gactacaaat
      601 atctgcagaa tggattcggt taataaaaaa aatgtttgag gaattaattt tcgctttgga
      661 gtggaggcct caattcgatg ttgcagattt tacaagtttt taaaatgtat ttcattatta
      721 ctttttatat gcctaataaa aaagccatag tttaatctat agataacttt ttttccagtg
      781 cactaacgga cgttacattc ccatacaaaa ctgcgtagtt aaagctaagg aaaagttaat
      841 atcatgttaa ttaaatacgc tatttacaat aagacattga actcatttat atcgttgaat
      901 atgaataacc aatttcagcg aatttttaac aaacatcgtt cacctcgttt aaggatatct
      961 tgtgtatggg gtgttgactt gctttatcga ataattaccg tacctgtaat tggcttgctg
     1021 gatatagcgg tagtctaata tctagcaaaa atcttttggg tgaaaaggct tgcaatttca
     1081 cgacaccgaa ctatttgtca ttttttaata aggaagtttt ccataaattc ctgtaattct
     1141 cggttgatct aattgaaaag agtagttttg catcacgatg aggagggctt ttgtagaaag
     1201 aaatacgaac gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc ccttacctga
     1261 agagtcgaat tttattgatg aacttataac ttccaagcat gcaaaccaaa agggagaaca
     1321 agtaatccaa gtagacacgg gaattggatt cttggatcac atgtatcatg cactggctaa
     1381 acatgcaggc tggagcttac gactttactc aagaggtgat ttaatcatcg atgatcatca
     1441 cactgcagaa gatactgcta ttgcacttgg tattgcattc aagcaggcta tgggtaactt
     1501 tgccggcgtt aaaagatttg gacatgctta ttgtccactt gacgaagctc tttctagaag
     1561 cgtagttgac ttgtcgggac ggccctatgc tgttatcgat ttgggattaa agcgtgaaaa
     1621 ggttggggaa ttgtcctgtg aaatgatccc tcacttacta tattcctttt cggtagcagc
     1681 tggaattact ttgcatgtta cctgcttata tggtagtaat gaccatcatc gtgctgaaag
     1741 cgcttttaaa tctctggctg ttgccatgcg cgcggctact agtcttactg gaagttctga
     1801 agtcccaagc acgaagggag tgttgtaaag atgaattgga ttatgtcagg aaaagaacga
     1861 caattttgca tccaaattgt ctaaatttta gagttgcttg aaaacaatag aaccttactt
     1921 gctttataat tacgttaatt agaagcgtta tctcgtgaag gaatatagta cgtagccgta
     1981 taaattgaat tgaatgttca gcttatagaa tagagacact ttgctgttca atgcgtcgtc
     2041 acttaccata ctcactttat tatacgactt taagtataaa ctccgcggtt atggtaaaat
     2101 taatgatgca caaacgtccg attccatatg ggtacactac aattaaatac ttttaagtaa
     2161 cgctgatttc ttatttttta tccttggtgt atttattgtt tataccgcta gggcggtgtg
     2221 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     2281 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2341 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2401 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     2461 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     2521 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     2581 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     2641 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2701 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     2761 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     2821 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     2881 gcgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga ggatctacga
     2941 taatgagacg gtgtttgggt tgtttttact gaatttttct tttattttat gacatcttaa
     3001 tttttatgtt aatgattatt cttatacttt tatctatctt tgattaaaac taatattcat
     3061 tcttttgatc actaattatt tttatagtat acgattcaac ccaactataa tacattgtat
     3121 caagagaaat ttttaattag atgtaattta atagccattt gataaaaaaa aattaccttt
     3181 ttttttgcta gtgttaaatt attgatcata acaatgtcga atgaatacta ataaaatagg
     3241 gacacgcgag ttgcaaatac ttgaaatacc tttgaagatt tgtctgtgaa ttttgataag
     3301 atttttatga ccatctcttt tatattcgat atcgtgaact ttcattttct tttgtttttt
     3361 gcttttcaag ttaaataacg cggaaattat atttccttat atgtttataa gagcttttaa
     3421 aggatttgct atatcgctta cagtactaca accggttgct caatgttatc cgtttccgcg
     3481 gtcttcttcc gtgcgcattc tgccgtgaag tgctaacgct gtgtgtggct ttatcaaaaa
     3541 agtggctcaa atatagcaat ttcatacata tttaaacacc cattatactt aaatgtatat
     3601 tttaaaatat ataagagatt ttcactgtat aactgtgtat cttatctata tgtaatcaac
     3661 ggcttcatac cacctcagcc agccgtgtta taacttaccg tttaccaact acattttttg
     3721 taacgaacca aaaaaccctc aaaagacaag accatgggag gtcccgggac tagtgcaagt
     3781 attaccaata ctggaaacga aaccatgaac cttccacaga tgcggtcgat ttggctggat
     3841 gaagatgaag aagctgaaaa actctacggt ctgcaggccc agcaattcat gggatctgat
     3901 gatgaagaaa acctaggcat tactttcatc aacagcgata aacctgtgct gagtaacaag
     3961 aaaaacattg agttgcctcc actttcacca aattcacatc cgtcttgcca ccacaggaga
     4021 agtaattcta actctgcaaa gtctaaagaa tcatcgtcat catcgtccag cgccaacaag
     4081 acaaatcaca aaaaggtttt ccttaagctt aatttgttga agaaaaagtt gcttggtgcc
     4141 caaccggaca taagaggtaa aggtatctcc acaccatttg attttcaaca tatttcacat
     4201 gctgacacta gaaatggatt ccaagatgag caattgcagg aaccttcatc gctgtccaca
     4261 gagattaagg acgactatac ctcctcctca agcaagcggg attcgaaatt aattaatatg
     4321 gtttcaaaag gcgaggaaga caatatggcc attataaagg aattcatgcg atttaaggtc
     4381 catatggagg gttcagtcaa tggccacgaa ttcgaaattg agggagaagg cgagggccgt
     4441 ccatatgagg gcacccaaac tgcaaagttg aaagtcacta agggaggccc cttaccattt
     4501 gcctgggaca tcttatcacc ccaattcatg tatggctcta aggcatatgt caagcatccc
     4561 gctgacatac ccgattactt gaaattatca ttccccgaag gcttcaaatg ggagcgtgta
     4621 atgaatttcg aagacggcgg cgttgtaacc gtcactcaag actcaagttt gcaagacggt
     4681 gaattcatat ataaggtcaa attgcgtgga accaactttc cctctgacgg acccgtcatg
     4741 caaaagaaaa ctatgggatg ggaggccagt tctgaaagaa tgtaccccga agacggcgct
     4801 cttaaaggcg agatcaaaca gcgtcttaaa ttgaaggatg gcggtcacta tgacgcagaa
     4861 gtcaagacca cttataaggc aaaaaagccc gtccaattac caggcgccta caatgttaac
     4921 ataaaattgg acatcacttc acataacgaa gactatacca tcgttgagca atacgaaaga
     4981 gccgagggcc gtcattcaac cggcggtatg gacgaacttt acaaaagtgg agcatcaggc
     5041 gcctccggtt ctggagtaag taaaggtgaa gaggacaaca tggcaataat caaagaattt
     5101 atgcgtttca aggtacacat ggaaggaagt gttaatggac atgagttcga gatagaaggc
     5161 gaaggtgaag gaagacccta cgagggtaca cagacagcca aattaaaagt aacaaaaggc
     5221 ggtccacttc ccttcgcttg ggacatactt agtcctcagt ttatgtatgg atcaaaagcc
     5281 tacgttaagc acccagcaga tatccctgat tatcttaagc ttagtttccc tgagggattt
     5341 aaatgggaaa gagtcatgaa cttcgaggat ggtggagtag tcactgtaac acaagatagt
     5401 tctcttcagg acggagagtt tatctataaa gttaagctta gaggcactaa cttcccatca
     5461 gatggccctg taatgcaaaa aaagacaatg ggctgggagg cttcatcaga gcgaatgtat
     5521 cctgaagatg gagcattgaa gggtgagata aagcaaagat tgaaattaaa agacggagga
     5581 cactacgatg ccgaagttaa aacaacatac aaggccaaga aacctgtaca acttcccggt
     5641 gcatataacg taaacatcaa gttagatata acaagtcata atgaggacta cactatagta
     5701 gaacaatatg agcgagctga gggaagacac agtactggag gaatggatga attgtataag
     5761 gcaggctcag gttccggtgc atctggcagt gtctctaagg gagaggagga taatatggct
     5821 atcattaaag agttcatgag attcaaagtt catatggaag gctctgtaaa cggtcacgag
     5881 tttgaaatcg aaggtgaggg agagggtcga ccttacgaag gaactcaaac cgctaaactt
     5941 aaggttacca agggtggacc tttgcctttc gcatgggata ttttgtctcc acaatttatg
     6001 tacggtagta aggcttacgt aaaacatcct gccgatattc cagactactt aaagttgtct
     6061 tttccagaag gttttaagtg ggagcgagtt atgaactttg aagatggagg tgtcgttaca
     6121 gttacccagg actcttcatt acaggatggc gaatttattt acaaggtaaa gttacgaggt
     6181 acaaattttc ctagtgatgg tccagttatg cagaagaaga ccatgggttg ggaagcatct
     6241 agtgaacgta tgtatccaga ggacggtgcc ttaaagggag aaattaaaca acgattaaag
     6301 cttaaggacg gtggccatta tgatgctgag gtaaagacta cctacaaagc taaaaaacca
     6361 gttcagttgc ctggagctta caacgtcaat attaaacttg atattacctc tcacaacgag
     6421 gattatacaa ttgtcgaaca gtacgagcgt gcagaaggtc gacattctac aggtggcatg
     6481 gatgagttat acaagtcgcg ataaggcgcg ccagatctgt ttagcttgcc tcgtccccgc
     6541 cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
     6601 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
     6661 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
     6721 cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
     6781 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
     6841 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
     6901 catgcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     6961 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     7021 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     7081 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     7141 gaacaggggc atcttgagcc cctgcggacg gtgtcgacag gtgcttctcg atctgcatcc
     7201 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     7261 attgctgccc tctggttatg tgtgggaggg ctaatcagta ctgacaataa aaagattctt
     7321 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     7381 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     7441 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg
     7501 tcgattcgat actaacgccg ccatccagtt taaacgagct ccagcttttg ttccctttag
     7561 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7621 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7681 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7741 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7801 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7861 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7921 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8161 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8221 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8401 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8641 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8701 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8761 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8821 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8881 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8941 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9001 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9061 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9121 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9181 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9241 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9301 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9361 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9421 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9481 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9541 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9601 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9661 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9721 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9781 tataaaaata ggcgtatcac gaggcccttt cgtc
//
